№ files_lp_4_process_3_136921
Supplementary tables and figure presenting experimental fish diet compositions, nutritional analyses, nucleotide primer sequences used for real-time PCR gene expression assays, and stability values of reference genes measured in the intestine of Megalobrama amblycephala.
Document type: Supplementary scientific material
Scientific field: Molecular biology; aquaculture nutrition
Research topic: Dietary carbohydrate levels and thiamine supplementation
Organism studied: Megalobrama amblycephala
Content elements: Experimental diet formulations; proximate composition analysis; nucleotide primer sequences; reference gene stability analysis
Methods referenced: Real-time PCR gene expression assay
Genes analyzed: AMPKα1, AMPKα2, PGC-1α, PGC-1β, TFAM, Mfn-1, Opa-1, Drp-1, Fis-1, Mff, ND-1, CYTB, COX-1, COX-2, SGLT-1, GLUT-2, EF1α, β-globin
Diet groups: C (control), CT (control + thiamine), HC (high-carbohydrate), HCT (high-carbohydrate + thiamine)
Nutritional parameters reported: Moisture, crude lipid, ash, crude protein, crude fiber, nitrogen-free extract, energy
Laboratory techniques: Nutritional composition analysis; primer-based gene expression quantification
Associated figure: Reference gene stability values (M values) in intestinal tissue
Biological sample: Intestine of Megalobrama amblycephala
Price: 8 / 10 USD
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