№ lp_2_1_12727
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Normative technical guidance chapter outlining principles and statistical approaches for estimating measurement uncertainty in quantitative diagnostic assays within veterinary laboratory quality standards.
Chapter:
2.2.4
Manual:
Terrestrial Manual
Related Chapters:
1.1.6 Principles and methods of Validation of diagnostic assays for infectious diseases of terrestrial animals; 2.2.6 Selection and use of reference samples and panels
Standard Referenced:
ISO/IEC 17025:2005; ISO/IEC 17025:2017
Issuing Organization:
World Organisation for Animal Health (WOAH)
Subject:
Estimation of measurement uncertainty in quantitative diagnostic tests
Scope:
Diagnostic assays for infectious diseases of terrestrial animals
Test Types Mentioned:
ELISA; RT-PCR
Approach Described:
Top-down (control sample) method
Statistical Measures:
Mean ± 2 SD; Relative Standard Deviation; Coefficient of Variation
Application Context:
Cut-off (threshold) values in serology and molecular diagnostics
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The product description is provided for reference. Actual content and formatting may differ slightly.
Year:
2026
Region / City:
Santa Maria, RS, Brazil
Topic:
Photobiological properties, antimicrobial assays
Document type:
Research Article
Organization / Institution:
Universidade Federal de Santa Maria - UFSM
Author:
Morgana Maciél Oliveira, Gabrielle Aguiar Dantas, Luiz Antônio Sodré Costa, Roberto Christ Vianna Santos, Bernardo A. Iglesias
Target Audience:
Researchers in photobiology and microbiology
Date of approval:
2026
Date of changes:
N/A
Note:
Contextual Description
Year:
2009
Region / city:
Oyster Pond, MA, USA
Subject:
Genetics, Microbiology
Document type:
Table
Organization / institution:
Not specified
Author:
Orata et al. (2015)
Target audience:
Researchers in the fields of microbiology, genetics, and epidemiology
Period of validity:
Not specified
Date of approval:
Not specified
Date of changes:
Not specified
Year:
2010
Region / City:
N/A
Subject:
Biomedical Research, Cell Biology, Molecular Biology
Document Type:
Research Methods Supplement
Author:
N/A
Target Audience:
Researchers, Scientists in Biomedical Fields
Period of Validity:
N/A
Approval Date:
N/A
Date of Changes:
N/A
Year of completion:
2023
Field:
Cancer research and tumor immunology
Research focus:
PPP2R2A deletion and expression in NSCLC and immunotherapy response
Type of document:
Supplementary materials and methods section of a scientific study
Data sources:
cBioPortal Pan-Lung cancer dataset; TCGA pan-cancer RNA-Seq data via Xena Browser; Kmplot survival database
Cell lines:
A549 cells; CMT167 PPP2R2A heterozygous knockout cells
Animal models:
CD11b-DTR mice (Jackson Laboratory, strain 006000); C57BL6 mice
Experimental techniques:
RNA sequencing; Gene Set Enrichment Analysis (GSEA); glycosylation assays with PNGase F and Endo H; adoptive Treg transfer; flow cytometry; Treg suppression assay with CellTrace Violet; ELISA
Repositories:
NCBI GEO (accession number GSE311238)
Analytes:
PD-L1, TNF-α, IFN-γ, IFN-α, IFN-β
Antibodies and reagents:
Anti-Mouse PD-L1 In Vivo Antibody (Clone 10F.9G2, Ichorbio); reagents from BioLegend, STEMCELL Technologies, eBioscience, R&D Systems, PBL Assay Science
Cited works:
Gyorffy B., 2024, Innovation (Camb); Qiu Z. et al., 2020, Cancer Research; Zhou L. et al., 2024, Journal of Clinical Investigation
Year:
2024
Region / city:
India
Topic:
Immunology, Biotechnology
Document type:
Book Chapter
Institution:
Tomo Riba Institute of Health & Medical Sciences (TRIHMS)
Author:
Ashmita Banik
Target audience:
Scientific community, Researchers in Biotechnology and Immunology
Period of validity:
N/A
Approval date:
N/A
Date of changes:
N/A
Year:
2024
Region / City:
Chennai
Subject:
Toxicology, Environmental Pollution, Microplastics
Document Type:
Research Article
Organization / Institution:
Not specified
Author:
Not specified
Target Audience:
Researchers, Environmental Scientists
Period of Validity:
Not specified
Approval Date:
Not specified
Date of Amendments:
Not specified
Procedure ID:
PSAv1.0
Version number:
1.0
Release date:
20 March 2017
Developed by:
Benoit Witkowski & Didier Ménard
Institution:
Unité d’Epidémiologie Moléculaire du Paludisme, Institut Pasteur du Cambodge
Location:
Phnom Penh, Cambodia
Document type:
Laboratory procedure
Purpose:
Evaluation of Plasmodium falciparum susceptibility to piperaquine
Assay types:
In-vitro Piperaquine Survival Assay (PSA); Ex-vivo Piperaquine Survival Assay
Target organism:
Plasmodium falciparum
Drug tested:
Piperaquine (PPQ)
Drug concentration:
200 nM
Exposure duration:
48 hours
Assessment time point:
72 hours after drug exposure
Intended users:
Appropriately-equipped laboratories performing in-vitro and ex-vivo drug susceptibility testing
Quality requirements:
Training required; participation in QA/QC proficiency scheme recommended
Referenced study:
Duru et al. 2015
Contact information:
[email protected]
Document type:
Supplementary material
Subject area:
Molecular biology and cancer research
Biological focus:
MIR22HG overexpression in cervical cancer cell lines
Cell lines:
HeLa; SiHa; C-33 A (HPV-)
Experimental methods:
Cell culture; transient transfection; RNA extraction; qRT-PCR; CCK-8 assay; Transwell migration and invasion assays
Genes referenced:
MIR22HG; GAPDH
Reagents and materials:
DMEM; MEM; FBS; Lipofectamine 2000; TRIzol; SYBR Green PCR kit; Matrigel
Data included:
Figure S1; Table S1; Table S7
Dataset description:
Annotated list of 4072 lncRNAs from HGNC database
Vendors mentioned:
Keygen Biotech (Nanjing, China); GeneChem (Shanghai, China); HyClone (Logan, UT, USA); Invitrogen (Carlsbad, CA, USA); Toyobo (Osaka, Japan); Beyotime (Shanghai, China); BD (Franklin Lakes, NJ, USA); Corning (New York, USA)
Year:
2023
Region / City:
N/A
Subject:
Genomic analysis, liver organoids
Document Type:
Research Methodology
Organization / Institution:
N/A
Author:
N/A
Target Audience:
Researchers, genomic scientists
Period of Validity:
N/A
Approval Date:
N/A
Date of Changes:
N/A
Year:
2026
Region / City:
Japan
Subject:
Immunology, Gene editing, Chemokine receptors
Document type:
Supplementary material / Methods
Institution:
FASMAC, Takara Bio, Integrated DNA Technologies, Thermo Fisher Scientific
Authors:
Not specified
Target audience:
Researchers in molecular biology and immunology
Experimental models:
HEK293T, Rat-1, KHYG-1, Ramos, CHO/G16 cells; hCCR8 knock-in mice
Techniques:
Gene transfection, CRISPR/Cas9 knock-in, flow cytometry, in vitro binding assays
Reagents:
Antibodies for human and mouse chemokine receptors, Puromycin, G418
Period of study:
Not specified
Date of preparation:
2026
Year:
2026
Field:
Nanotechnology / Analytical Chemistry
Document type:
Research supplementary material
Techniques:
Transmission Electron Microscopy (TEM), HAADF-STEM, CL dot-immunobinding assay
Materials:
Pyramidal platinum nanoparticles, Rabbit-IgG
Calibration range:
PtNPs 0.04–4 μg/mL, Rabbit-IgG 0.08–0.5 ng/mL
Instruments:
JEOL JEM-1011, JEOL JEM-2200FS
Measurements:
Triplicate for calibration curves
Data presentation:
TEM images, calibration graphs
Target audience:
Researchers in nanomaterials and analytical chemistry
Year:
2016
Institution:
University of Wisconsin–Madison
Document type:
Request for Bid / Contract Solicitation
Contract number:
17-5725-R1
Issue date:
8/17/16
Due date:
8/31/16, 2:00 PM CDT
Agent:
Janet Bresnahan
Scope:
In Vivo Drug Discovery Pharmacology Assays, Pharmacokinetics and Dose Tolerability in Rodents
Contract duration:
One year from award, automatically renewable for two additional one-year terms
Bid submission requirements:
Hard copy, CD/DVD/Flash drive, signatures required
Applicable rules:
Federal procurement rules, University standard terms and conditions
Sections included:
Submittal Instructions, General Information, RFB Process Instructions, Bidder Qualifications, Performance and Contract Requirements, Attachments A–H
Audience:
Potential vendors and contractors for pharmacology research services
Contextual description:
Official bid solicitation detailing requirements, procedures, and conditions for vendors to provide in vivo pharmacology testing services at the University of Wisconsin–Madison under a specified contract.
Research Field:
Immunology
Subfield:
Host Defense and Sepsis
Experimental Model:
C57BL/6 and C57BL/6J mice
Biological Focus:
Macrophage function and bacterial clearance
Bacterial Species:
Pseudomonas aeruginosa
Laboratory Techniques:
CFU determination, ELISA, flow cytometry, Western blot, siRNA interference, fluorescence microscopy
Cell Type:
Mouse peritoneal macrophages (PMφs)
Tissues Examined:
Lung, liver, spleen, kidney
Biochemical Markers:
ALT, AST, LDH, creatinine
Cytokines and Chemokines Measured:
Prokineticin-2, TNF-α, IL-6, IL-10, IL-17A
Experimental Interventions:
rPK2 stimulation, PKR1/PKR2 siRNA transfection, macrophage depletion with clodronate liposomes
Time Points of Analysis:
24 hours and 48 hours after modeling
Detection Methods:
Hematoxylin-eosin staining, fluorescence microscopy, FACScan flow cytometry, ELISA assays
Software for Analysis:
FCM Express
Referenced Studies:
Song et al., 2016; Song et al., 2015
Animal Age:
Six- to eight-week-old mice
Note:
Year
Theme:
Microbiology, Pathogen Studies
Document Type:
Scientific protocol
Target Audience:
Researchers, Laboratory Professionals
Year:
2002–2009
Institution:
Massachusetts Institute of Technology
Type of Document:
Academic article
Author:
Lindsey Weeramuni
Correspondence:
[email protected]
Keywords:
fair use, open educational resources, training, Codes of Best Practices, Creative Commons
Scope:
OpenCourseWare / Online educational resources
Context:
United States
Target Audience:
Educators, academic institutions, OER practitioners
Year:
2023
Region / City:
Geneva
Topic:
Measurement Uncertainty, Vehicle Regulations, Noise and Tyres
Document Type:
Proposal
Organization / Institution:
United Nations, Economic Commission for Europe, Inland Transport Committee, World Forum for Harmonization of Vehicle Regulations
Author:
Informal Working Group on Measurement Uncertainties
Target Audience:
Members of the Working Party on Noise and Tyres, stakeholders in vehicle regulation and testing
Period of Validity:
Ongoing
Approval Date:
07 February 2023
Date of Changes:
N/A
Year:
2023
Region / City:
International
Topic:
Measurement Uncertainty, Vehicle Regulations
Document Type:
Proposal
Organization / Institution:
Informal Working Group on Measurement Uncertainties (IWG MU)
Author:
Chair of the Informal Working Group on Measurement Uncertainties (IWG MU)
Target Audience:
Experts in vehicle regulations and measurement uncertainty
Period of Validity:
Not specified
Approval Date:
7-10 February 2023
Date of Changes:
Proposed for Revision 2
Year:
2023
Location:
Ishikawa, Japan
Event Type:
Conference
Organizing Institution:
Japan Advanced Institute of Science and Technology (JAIST)
Target Audience:
Researchers, Academics, Practitioners in Knowledge Modelling, Decision Making, and Uncertainty
Period:
November 2 – 4, 2023
Date of Approval:
Not specified
Date of Changes:
Not specified
Contextual Description:
A scientific conference focused on uncertainty in knowledge modelling, decision making, and various related fields.
Date:
July 25th, 2016
Amended:
August 18th, 2016, Further Amended October 11th, 2016, December 19th, 2016, January 13th, 2017, January 20th, 2017, Further Amended August 2/3/5/6/9, 2019, Further Amended August 15, 2019, Further Amended August 20/21/22, 2019, Further Amended May 14, 2020, Further Amended November 22nd and 23rd, 2021, February 28th , March 1st, March 9th/10th/11th, March 15th/16th/17th and 28th, April 4th/5th, 8th, 15th, 22nd, 25th, May 23rd, 24th, 26th and 30th and June 3rd and 15th/16th/17th, 2022, end notes added January 25, 2021 and updated September 7th, 2021, further updated February 22nd, 2022 and removed May 30th, 2022.
Author:
Louis Charles Taylor
Region / City:
N/A
Subject:
Philosophy of Physics, Theoretical Physics
Document Type:
Research Paper
Institution:
N/A
Audience:
Theoretical physicists, philosophers of science
Period of Validity:
N/A
Approval Date:
N/A
Modification Dates:
August 2-3, 2019, August 15, 2019, August 20-22, 2019, May 14, 2020, November 22-23, 2021, February 28, 2022, March 1-28, 2022, April 4-25, 2022, June 3-15, 2022
Endnotes Added:
January 25, 2021
Last Update:
May 30, 2022