№ files_lp_3_process_7_021113
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This document provides supplementary information on various experimental methods, including RNA isolation, antibody usage, and cell migration assays, in the context of biomedical research.
Year:
2010
Region / City:
N/A
Subject:
Biomedical Research, Cell Biology, Molecular Biology
Document Type:
Research Methods Supplement
Author:
N/A
Target Audience:
Researchers, Scientists in Biomedical Fields
Period of Validity:
N/A
Approval Date:
N/A
Date of Changes:
N/A
Price: 8 / 10 USD
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Note:
Year
Region / City:
United States
Topic:
Pharmaceutical naming and regulatory processes
Document type:
Application form
Organization / Institution:
United States Adopted Names Council, American Medical Association
Target audience:
Pharmaceutical companies, researchers, regulatory authorities
Year:
Not specified
Region / city:
Not specified
Topic:
Antibody usage in immunology study
Document type:
Table
Organization / institution:
Not specified
Author:
Not specified
Target audience:
Researchers in immunology
Period of validity:
Not specified
Approval date:
Not specified
Modification date:
Not specified
Year:
2025
Region / City:
Northern Victoria
Topic:
Epidemiology, Infectious Diseases, Public Health
Document Type:
Research Study
Author:
Marie Heloury, Joshua Szanyi, Maxwell Braddick, Alexander Fidao, Madeleine J Marsland, Tilda N Thomson, Mitch Batty, Suellen Nicholson, Theo Karapanagiotidis, Kylie Carville, Anna-Jane Glynn-Robinson, Chuan Kok Lim, Naveen Tenneti, Anthony Zheng, William Cross, Jim Black, Helen O’Brien
Target Audience:
Researchers, Public Health Officials, Epidemiologists
Period of Validity:
2023–2024
Date of Approval:
2025-03-25
Date of Changes:
Not specified
Year:
2024
Region / city:
India
Topic:
Immunology, Biotechnology
Document type:
Book Chapter
Institution:
Tomo Riba Institute of Health & Medical Sciences (TRIHMS)
Author:
Ashmita Banik
Target audience:
Scientific community, Researchers in Biotechnology and Immunology
Period of validity:
N/A
Approval date:
N/A
Date of changes:
N/A
Section:
Supplemental Methods
Field:
Immunology and Hematologic Malignancies
Experimental focus:
B7-H3 CAR-T cells in acute myeloid leukemia (AML)
Monoclonal antibodies:
Anti-human CD markers and related reagents for flow cytometry
Suppliers:
Becton-Dickinson (BD) Biosciences; Miltenyi Biotec; BioLegend; Jackson ImmunoResearch Labs
Cell lines:
THP1; U937; HL-60; Kasumi-1; OCI-AML2; OCI-AML3
Primary samples:
DFAM-6855; CBAM-44728 from Public Repository of Xenografts (PRoXe), Dana-Farber Cancer Institute
Biological material:
Primary AML blasts; donor PBMCs; buffy coats from healthy volunteers
Vector system:
SFG retroviral vector with MoMLV gag-pol and RD114 envelope
Transduction method:
Retronectin-coated plates with retroviral supernatant
Cell expansion cytokines:
IL7; IL15
Statistical methods:
Mann Whitney test; one-way ANOVA with Holm-Sidak adjustment; Chi-square test
Software:
Prism version 8 (GraphPad)
Sample origin:
Bone marrow and peripheral blood from AML patients
Animal model:
NSG mice for in vivo expansion of xenografts
Culture conditions:
RPMI-1640 medium with fetal bovine serum, L-glutamine, penicillin, and streptomycin
Year:
2026
Region / City:
Not specified
Subject:
Clinical diagnostics, virology
Document Type:
In vitro diagnostic test instructions
Organization:
ZEUS Scientific, Inc.
Author:
Not specified
Target Audience:
Laboratory personnel
Period of Use:
Ongoing
Date of Issue:
2026
Safety Precautions:
Biosafety Level 2, toxic and irritant reagents handling instructions included
Components:
Microplate strips, conjugate, controls, calibrator, diluent, substrate, stop solution, wash buffer
Intended Sample:
Human serum
Test Principle:
ELISA detection of IgG antibodies to EBV Nuclear Antigen 1 (EBNA-1)
Assay Procedure:
Three-step incubation with antigen-coated wells, conjugate, and substrate followed by photometric measurement
Study title:
Prevalence of Pathogenic Antibodies in Psychosis 2 (PPiP2)
Principal Investigator:
Local PPiP2 researcher
Participant study ID:
[ID]
Date:
[Date]
Version:
[Version]
Year:
[Year]
Region / City:
[Region or city if mentioned]
Topic:
Medical Research, Psychiatric Studies
Document Type:
Consent Form
Institution:
University of Oxford
Author:
[Author(s) if available]
Target Audience:
Research Participants, Medical Professionals
Study Period:
[Study Period if mentioned]
Approval Date:
[Date of approval if mentioned]
Amendment Date:
[Date of amendments if mentioned]
Year:
Not specified
Region / city:
Not specified
Topic:
Environmental, Social, Economic Impacts of Chemical & Pharmaceutical Products
Document Type:
Environmental/Social/Economic Impact Analysis
Organization / Institution:
Not specified
Author:
Not specified
Target Audience:
Not specified
Effective Date:
Not specified
Date of Changes:
Not specified
Contextual description:
Environmental, social, and economic impact analysis of chemical and pharmaceutical products.
Year:
2026
Region / City:
Not specified
Field:
Immunology / Molecular Biology
Document Type:
Supplementary Table
Institution:
Not specified
Authors:
Not specified
Target Audience:
Researchers in immunology and molecular biology
Reagents Included:
Antibodies, Chemicals, Peptides, Recombinant Proteins, Additional Reagents, Critical Commercial Assays
Organism:
Mouse
Experimental Context:
T cell characterization and immunological assays
Source:
Multiple commercial suppliers listed for each reagent
Note:
Year
Year:
2021
Institution:
Niigata University
Type of document:
Research protocol and experimental report
Target molecules:
GlyR (GLRA1), GABABR (GABBR1), NMDAR, AMPAR, LGI1, GABAAR, CASPR2
Methodology:
PCR amplification, In-Fusion HD cloning, cell-based assay (CBA), fluorescence microscopy
Sample type:
Serum, cerebrospinal fluid (CSF)
Patient group:
55 SPS patients, neurodegenerative disorder controls, healthy volunteers
Assay validation:
Control samples tested negative
Reference studies:
Dalakas MC et al., Tanaka K et al., Nakane S et al., Nakanishi T et al., Maeda K et al., Iizuka T et al., Nanaura H et al.
Year:
2024
Region / City:
Europe
Topic:
Preclinical drug safety assessment
Institution:
Master’s programme, Drug Innovation
Author:
Benedetta Boscolo Todaro
Supervisors:
Hsiao-Tzu Chien, Marieke de Bruin, Peter Van Meer
Target audience:
Regulatory scientists, pharmacologists
Study period:
October 2024 – April 2025
Data analyzed:
42 monoclonal antibodies submitted for EMA approval (2017–2024)
Methodology:
Weight-of-evidence approach, literature review, short-term toxicity studies
Ethical considerations:
Reduction of animal testing, alignment with 3Rs principles
Chapter:
2.2.4
Manual:
Terrestrial Manual
Related Chapters:
1.1.6 Principles and methods of Validation of diagnostic assays for infectious diseases of terrestrial animals; 2.2.6 Selection and use of reference samples and panels
Standard Referenced:
ISO/IEC 17025:2005; ISO/IEC 17025:2017
Issuing Organization:
World Organisation for Animal Health (WOAH)
Subject:
Estimation of measurement uncertainty in quantitative diagnostic tests
Scope:
Diagnostic assays for infectious diseases of terrestrial animals
Test Types Mentioned:
ELISA; RT-PCR
Approach Described:
Top-down (control sample) method
Statistical Measures:
Mean ± 2 SD; Relative Standard Deviation; Coefficient of Variation
Application Context:
Cut-off (threshold) values in serology and molecular diagnostics
Year:
2026
Region / City:
Santa Maria, RS, Brazil
Topic:
Photobiological properties, antimicrobial assays
Document type:
Research Article
Organization / Institution:
Universidade Federal de Santa Maria - UFSM
Author:
Morgana Maciél Oliveira, Gabrielle Aguiar Dantas, Luiz Antônio Sodré Costa, Roberto Christ Vianna Santos, Bernardo A. Iglesias
Target Audience:
Researchers in photobiology and microbiology
Date of approval:
2026
Date of changes:
N/A
Note:
Contextual Description
Year:
2009
Region / city:
Oyster Pond, MA, USA
Subject:
Genetics, Microbiology
Document type:
Table
Organization / institution:
Not specified
Author:
Orata et al. (2015)
Target audience:
Researchers in the fields of microbiology, genetics, and epidemiology
Period of validity:
Not specified
Date of approval:
Not specified
Date of changes:
Not specified
Year of completion:
2023
Field:
Cancer research and tumor immunology
Research focus:
PPP2R2A deletion and expression in NSCLC and immunotherapy response
Type of document:
Supplementary materials and methods section of a scientific study
Data sources:
cBioPortal Pan-Lung cancer dataset; TCGA pan-cancer RNA-Seq data via Xena Browser; Kmplot survival database
Cell lines:
A549 cells; CMT167 PPP2R2A heterozygous knockout cells
Animal models:
CD11b-DTR mice (Jackson Laboratory, strain 006000); C57BL6 mice
Experimental techniques:
RNA sequencing; Gene Set Enrichment Analysis (GSEA); glycosylation assays with PNGase F and Endo H; adoptive Treg transfer; flow cytometry; Treg suppression assay with CellTrace Violet; ELISA
Repositories:
NCBI GEO (accession number GSE311238)
Analytes:
PD-L1, TNF-α, IFN-γ, IFN-α, IFN-β
Antibodies and reagents:
Anti-Mouse PD-L1 In Vivo Antibody (Clone 10F.9G2, Ichorbio); reagents from BioLegend, STEMCELL Technologies, eBioscience, R&D Systems, PBL Assay Science
Cited works:
Gyorffy B., 2024, Innovation (Camb); Qiu Z. et al., 2020, Cancer Research; Zhou L. et al., 2024, Journal of Clinical Investigation
Year:
2024
Region / city:
India
Topic:
Immunology, Biotechnology
Document type:
Book Chapter
Institution:
Tomo Riba Institute of Health & Medical Sciences (TRIHMS)
Author:
Ashmita Banik
Target audience:
Scientific community, Researchers in Biotechnology and Immunology
Period of validity:
N/A
Approval date:
N/A
Date of changes:
N/A