№ files_lp_4_process_3_087856
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Methods section of an experimental biomedical study describing laboratory procedures used to quantify bacterial load, analyze tissue pathology, evaluate macrophage phagocytosis and intracellular killing, measure cytokines, and examine signaling pathways in murine models of infection and sepsis.
Research Field:
Immunology
Subfield:
Host Defense and Sepsis
Experimental Model:
C57BL/6 and C57BL/6J mice
Biological Focus:
Macrophage function and bacterial clearance
Bacterial Species:
Pseudomonas aeruginosa
Laboratory Techniques:
CFU determination, ELISA, flow cytometry, Western blot, siRNA interference, fluorescence microscopy
Cell Type:
Mouse peritoneal macrophages (PMφs)
Tissues Examined:
Lung, liver, spleen, kidney
Biochemical Markers:
ALT, AST, LDH, creatinine
Cytokines and Chemokines Measured:
Prokineticin-2, TNF-α, IL-6, IL-10, IL-17A
Experimental Interventions:
rPK2 stimulation, PKR1/PKR2 siRNA transfection, macrophage depletion with clodronate liposomes
Time Points of Analysis:
24 hours and 48 hours after modeling
Detection Methods:
Hematoxylin-eosin staining, fluorescence microscopy, FACScan flow cytometry, ELISA assays
Software for Analysis:
FCM Express
Referenced Studies:
Song et al., 2016; Song et al., 2015
Animal Age:
Six- to eight-week-old mice
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Year:
2023
Region / City:
Not specified
Topic:
Immunology, Cytotoxicity, Flow Cytometry
Document Type:
Research Supplementary Figures
Author:
Not specified
Target Audience:
Researchers, Immunologists
Period of Validity:
Not specified
Approval Date:
Not specified
Modification Date:
Not specified
Authors:
Jianhua Rao; Jiannan Qiu; Ming Ni; Hao Wang; Peng Wang; Lei Zhang; Zeng Wang; Mu Liu; Feng Cheng; Xuehao Wang; Ling Lu
Affiliations:
Hepatobiliary Center of The Affiliated Cancer Hospital (Jiangsu Cancer Hospital) and The First Affiliated Hospital, Nanjing Medical University; Research Unit of Liver Transplantation and Transplant Immunology, Chinese Academy of Medical Sciences; Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment; Collaborative Innovation Center for Personalized Cancer Medicine; State Key Laboratory of Reproductive Medicine
Institution:
Nanjing Medical University
Country:
China
Type of Document:
Supplementary Material
Discipline:
Immunology; Hepatology; Molecular Biology
Subject:
Role of macrophage Nrf2 deficiency and Timp3-mediated RhoA/ROCK pathway in ischemic liver injury
Experimental Models:
Mouse models; Bone marrow–derived macrophages (BMMs); Hepatic macrophages
Methods:
Cell isolation and culture; Biochemical measurements; Histopathology; Immunohistochemistry; Immunofluorescence; TUNEL staining; MDA, GSH and MPO assays; Western blotting; qRT-PCR; ELISA; RNA sequencing; Chromatin immunoprecipitation
Correspondence:
Ling Lu; Xuehao Wang; Feng Cheng
Equal Contribution:
Jianhua Rao; Jiannan Qiu; Ming Ni
Year:
2026
Species:
Mus musculus
Sex:
Male and Female
Strains:
LLC, NOD-SCID
Experimental procedure:
Transverse aortic constriction (TAC) with and without tumor implantation
Measured parameters:
Fractional shortening, ventricles/body weight ratio, mRNA levels of hypertrophic and fibrosis markers, macrophage polarization markers
Techniques:
Echocardiography, qRT-PCR, flow cytometry, immunohistochemistry
Statistical analysis:
Student t-test, results expressed as mean ± SEM
Markers analyzed:
ANP, BNP, ACTA1, Collagen, TGFβ3, CTGF, SMA, iNOS, CD206, ARG1, F4/80, CD163, INFγ, IL-13, CCL2, TNFα
Supplemental tables:
Oligonucleotide sequences, echocardiography parameters
Supplemental figures:
6 figures detailing cardiac function, macrophage polarization, and tumor growth effects
Year:
2026
Region / City:
Not specified
Topic:
Bone Regeneration, Macrophage Phenotype Switching
Document Type:
Research Article
Organization / Institution:
Not specified
Author:
Not specified
Target Audience:
Researchers, Medical Professionals
Duration:
Not specified
Approval Date:
Not specified
Modification Date:
Not specified
Year:
Not specified
Region / City:
Not specified
Theme:
Genetics, Oncology
Document Type:
Research Article
Organization / Institution:
Not specified
Author:
Not specified
Target Audience:
Researchers, Scientists, Medical Professionals
Period of Validity:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Year:
2021-2023
Region / City:
Germany, United Kingdom, Canada
Subject:
Immunology, Chronic Lymphocytic Leukemia
Document Type:
Research supplementary materials
Institution / Organization:
Various academic and research institutions
Authors:
Roessner PM, Llao Cid L, Lupar E, Hanna BS, Ozturk S, Chakraborty S, Martines C, Porro F
Target Audience:
Researchers and clinicians in hematology and immunology
Sample Size:
14 CLL patients
Patient Sex Distribution:
28.6% female (4/14)
Patient Age:
mean 63.3 years, median 59 years
Binet Stage Distribution:
A 64.3% (9/14), B 7.1% (1/14), C 28.6% (4/14)
IGHV Mutational Status:
92.9% mutated (13/14)
Chromosomal Aberrations:
del13q 85.7% (12/14), normal karyotype 14.3% (2/14), del14q 7.14% (1/14)
TP53 Mutation:
0.0% (0/14)
Prior Treatment:
0.0% (0/14)
Methodology:
Flow cytometry, T-cell isolation and activation, proliferation assays, cytotoxicity assays
Animal Models:
Eµ-TCL1 mice, P14xCD4-Cre LCMV TCR-transgenic mice
Reagents and Equipment:
Detailed suppliers and catalog numbers for antibodies, cell isolation kits, dyes, and culture materials
Context:
Experimental research data detailing procedures for isolating, stimulating, and analyzing T cells from CLL patients and murine models using flow cytometry and functional assays.
Year:
2026
Region:
Laboratory mouse model, C57BL6/KaLwRij
Subject:
Multiple myeloma, osteoblast biology, Runx2 gene function
Document type:
Research supplementary methods
Institution:
Academic research laboratory
Authors:
Not specified
Target audience:
Biomedical researchers, immunologists, oncologists
Experimental period:
Mouse model development and analysis up to 3 months of age
Methods:
Bioluminescence imaging, ELISA, cytokine array, osteoblast isolation and culture, ALP staining, Western blot, cell migration assays, flow cytometry
Genes studied:
Runx2, Samsn1
Cell types:
Osteoblasts, 5TGM1 multiple myeloma cells
Document type:
Supplementary scientific figures
Field:
Immunology
Research topic:
Dendritic cells, antigen uptake, T cell proliferation, and sarcosine effects in tumor models
Experimental model:
Murine bone marrow–derived dendritic cells (BM-DC) and B16F10-OVA tumor-bearing mice
Biological materials:
FITC-OVA antigen, OVA-mRNA electroporated dendritic cells, sarcosine treated dendritic cells
Methods:
Antigen uptake measurement by mean fluorescence intensity (MFI), in vitro T cell proliferation assay, co-culture experiments, tumor implantation and monitoring
Statistical analysis:
Unpaired t test; two-way ANOVA
Sample size:
n=3; n=5; n=10
Measured indicators:
Antigen uptake (MFI), T cell proliferation percentage, body weight, tumor volume
Experimental comparison groups:
Control BM-DC, untreated BM-DC with OVA-mRNA electroporation, sarcosine treated BM-DC with OVA-mRNA electroporation, sarcosine loaded tumor conditions
Organism:
Mouse
Period:
1620–1691
Region:
New England, Massachusetts
Topic:
Colonial American history
Type of document:
Educational historical overview
Historical subjects:
Plymouth Colony; Pilgrims; Puritans; Wampanoag
Key events:
Mayflower voyage; first winter; Mayflower Compact; 1621 treaty with Massasoit; incorporation into Massachusetts Bay Colony
Political concepts:
freedom of conscience; self-government; social covenant
Associated figures:
William Bradford; Massasoit; Tisquantum (Squanto)
Related colonies:
Jamestown; Massachusetts Bay Colony
Year:
1590
Location:
Roanoke Island
Topic:
Theories about the fate of the Lost Colony of Roanoke
Document type:
Historical theory
Institution:
None
Author:
Unspecified
Target audience:
Historians, students of American history
Time period:
1587-1590
Date of approval:
Not specified
Date of amendments:
Not specified
Contextual description:
A historical source analyzing multiple theories about the disappearance of the Lost Colony of Roanoke, discussing potential scenarios like Spanish involvement, starvation, Native American attacks, disease, and natural disasters.
Note:
Name
Title:
Mission 1: “For Crown or Colony?”
Website:
www.mission-us.org
Historical Setting:
Boston, 1770
Main Character:
Nat Wheeler
Related Event:
Boston Massacre
Adapted From:
Mission US: Flight to Freedom teacher guide
Associated Poem:
“On the death of Mr Snider Murder’d by Richardson” by Phillis Wheatley
Subject:
American Revolution; Patriots and Loyalists; Christopher Seider; Boston Massacre
Document Type:
Educational worksheet
Intended Audience:
Students
Activities:
Gameplay reflection; historical analysis; primary source comparison; poetry analysis
Year:
1732
Region / city:
Georgia
Document type:
Educational lesson plan
Institution:
Georgia State Curriculum
Author:
Unknown
Target audience:
Students
Period of validity:
1732 onward
Approval date:
Unknown
Revision date:
Unknown
Year:
2025
Region / City:
Continental Colony
Topic:
Budget, Strategic Priorities, Community Engagement
Document Type:
Meeting Minutes
Organization:
Continental Colony GO Team
Author:
Carolyn Strozier
Target Audience:
GO Team Members, Community Stakeholders
Period of Action:
February 13, 2025
Approval Date:
February 13, 2025
Amendment Date:
N/A
Year:
1607–1705
Region:
Virginia Colony, North America
Topic:
Slavery, Legal History
Document Type:
Educational Activity / Timeline
Institution:
Virginia Colony Archives / Historical Education Program
Target Audience:
Students / Independent Learners
Legal Focus:
Codification of race-based slavery
Content Format:
Interactive timeline with law summaries and analysis questions
Year:
2023
Location:
Harizan Colony, Pandu, Guwahati, India
Theme:
Gender equality, education, employment, health
Document type:
Field survey report
Institution:
Department of Economics, Pandu College
Supervisor:
Dr. Binita Tamuli Barman
Participants:
17 students
Objectives:
Assess gender equality in education, examine job market biases, investigate cultural and economic impacts on girls’ education, analyze gender-based violence, evaluate women’s healthcare
Outcomes:
Enhanced student research skills, increased educational access, reduced gender biases in employment, empowered girls, reduced gender-based violence, improved women’s health services
Date of survey:
22 December 2023
Note:
Year
Region / City:
South Australia
Document Type:
Application form
Institution / Organization:
University of Adelaide, Flinders University, SAHMRI, SA Pathology, University of South Australia, WCHN, PIRSA/SARDI
Purpose:
Scientific research or teaching
Species/Strain:
Various laboratory animals
Genetic Modification Status:
Specified for GM animals when applicable
Project Duration:
To be determined by applicant
Submission Method:
Online form with attached breeding colony form
Approval Required By:
Relevant Animal Ethics Committees
Contact Details:
Included for all applicants and co-applicants
Compliance:
Subject to Animal Welfare Act 1985 and other relevant permits or regulations
Expected Animal Use Start Date:
To be specified
Expected Animal Use End Date:
To be specified
Colony Housing:
Institution-based animal facilities
Welfare Considerations:
Steps to minimize welfare impact required
Training and Credentials:
All personnel must have relevant training and permits
Information Sharing Consent:
Yes/No as indicated by applicant
Year:
Early 18th century
Region / City:
Virginia Colony
Theme:
Education, Native American Perspective
Document Type:
Letter
Organization / Institution:
Virginia Colony
Author:
Canassatego
Target Audience:
Virginia Colony Leaders
Effective Period:
N/A
Approval Date:
N/A
Date of Changes:
N/A
Year:
2026
Country:
Pakistan
Region / City:
Lahore
Project ID:
0074-PAK
Type of Document:
Request for Expressions of Interest (Consulting Services – Firms Selection)
Implementing Agency:
Water and Sanitation Agency, Lahore (L-WASA)
Financing Institution:
Asian Infrastructure Investment Bank (AIIB)
Assignment Title:
Project Management Consultant (PMC) / Owner’s Engineer
Project Scope:
Laying 28 km trunk sewer and construction of pumping/disposal station of 350 cusecs capacity using trench-less technology
Target Audience:
Consulting firms with relevant experience in urban sewerage projects
Required Expertise:
Preliminary and detailed design, tendering and bidding, construction supervision, environmental and social management
Contract Conditions:
FIDIC Conditions of Contract compliance
Project Components:
Sewer line installation via trenchless technology, construction of pumping/disposal station, SCADA integration, restoration of existing drains
Project Duration:
Not specified
Submission Requirements:
Presentation of consultancy firm, financial statements, experience in similar projects, team qualifications
Year:
2026
Region / City:
Bar Harbor, Maine
Subject:
Mouse Colony Management
Document Type:
Workshop Justification Letter
Organization:
The Jackson Laboratory
Author:
[Name of the sender]
Target Audience:
Academic, biotech, and pharma professionals managing mouse colonies
Duration:
August 24 – 28, 2026
Approval Date:
[Approval date if mentioned]
Date of Changes:
[Date of changes if applicable]
Year:
2013
Institution:
University of North Carolina at Chapel Hill
Organization:
Kappa Phi Lambda Sorority Inc.
Document Type:
Bylaws
Purpose:
Establish governance, conduct, and membership rules for the sorority
Target Audience:
Members of the University of North Carolina at Chapel Hill Colony
Membership Requirements:
Minimum GPA 2.5, payment of dues, community service hours
Governance Structure:
Judicial Board, Executive Board, Minor Board
Meeting Schedule:
Determined at the start of each semester
Dues:
$200 per semester
Voting Rules:
Majority vote, secret ballots for official positions
Amendments:
Reviewed every semester
Community Service:
Minimum 15 hours per semester, including at least one group event
Code of Conduct:
Prohibits slander, discrimination, and divulging sorority secrets
Election Procedures:
Includes speech, Q&A, and pro/con sessions
Greek Alliance Council Membership:
Required adherence to council guidelines and participation in events