№ files_lp_4_process_3_087913
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This document provides detailed methods for preparing Clostridium difficile spores and conducting various assays, including toxin cytotoxicity and gene expression analysis in mice.
Note:
Year
Theme:
Microbiology, Pathogen Studies
Document Type:
Scientific protocol
Target Audience:
Researchers, Laboratory Professionals
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The product description is provided for reference. Actual content and formatting may differ slightly.
Year:
2023
Region / city:
Southern North Island
Theme:
Beekeeping, AFB Detection
Document Type:
Fundraising Proposal
Organization / Institution:
Southern North Island Beekeeping Group
Author:
Allan Richards
Target Audience:
Beekeepers, Honey Industry Stakeholders
Duration:
Ongoing
Approval Date:
N/A
Date of Changes:
N/A
Funding Amount:
$36,000 per year for dog training
Project Status:
Fundraising to maintain trained dogs
Additional Information:
AFB detection using sniffer dogs
Note:
Year
Theme:
Mycology
Document type:
Scientific article
Author:
D.Ebtihal Muiz
Target audience:
Researchers, students, biologists
Year:
2016
Region / City:
London, Swindon, Berkshire, Merseyside, Yorkshire, Sunderland
Topic:
Clostridium difficile infection, hospital infection control
Document Type:
Research article
Organization:
Guy’s & St Thomas’ NHS Foundation Trust, Great Western Hospitals NHS Foundation Trust, Heatherwood and Wexham Park Hospitals NHS Foundation Trust, St Helens & Knowsley Hospitals NHS Trust, Calderdale & Huddersfield NHS Foundation Trust, City Hospitals Sunderland NHS Foundation Trust
Author:
Not specified
Target Audience:
Healthcare professionals, researchers
Period of Validity:
Not specified
Approval Date:
Not specified
Modification Date:
Not specified
Context:
A research article detailing the comparison of C. difficile infection control measures across six hospitals in England using whole-genome sequencing to track transmission patterns.
Year:
2026
Region / City:
United States
Topic:
Healthcare, Infection Control, Long-Term Care Facilities
Document Type:
Guidelines
Organization:
Centers for Disease Control and Prevention (CDC)
Author:
Not specified
Target Audience:
Healthcare professionals in Long-Term Care Facilities (LTCFs)
Period of Validity:
Not specified
Approval Date:
Not specified
Date of Revisions:
Not specified
Year:
2024
Region / city:
United States
Theme:
Gastrointestinal pharmacology, infectious diseases, antibiotic resistance, healthcare management
Document Type:
Review
Institution:
Hackensack Palisades Medical Center, Augusta University
Author:
Nagesh VK, Tran HHV, Elias D, Kianifar Aguilar I, Sethi T, Menon A, Mansour C, Furman F, Tsotsos K, Subar T, Auda A, Sidiqui A, Lamar J, Wadhwani N, Dey S, Lo A, Atoot A, Weissman S, Sifuentes H, Bangolo AI
Target Audience:
Healthcare professionals, researchers, medical practitioners
Period of Validity:
Ongoing studies, review is relevant until new treatments emerge
Date of Approval:
2024-07-25
Date of Changes:
2024-07-21
Year:
2023
Region / city:
Minnesota
Topic:
Infection Prevention and Control
Document type:
Policy
Organization / institution:
Minnesota Department of Health
Author:
Unknown
Target audience:
Healthcare workers and medical staff
Period of validity:
Not specified
Approval date:
Not specified
Review date:
Not specified
Date of changes:
Not specified
Treatment protocols:
Polymerase chain reaction (PCR) testing preferred over enzyme immunoassay (EIA) tests
Environmental cleaning requirements:
Daily cleaning and disinfection of rooms
Hand hygiene requirements:
Soap and water preferred, alcohol-based hand rubs acceptable
Contact precautions:
Required for suspected CDI residents
Room placement:
Private rooms preferred
Laundry protocol:
Contaminated laundry to be disinfected
Treatment:
Antibiotic stewardship important
Notes:
Regular updates and specific protocols for testing and care
Year:
2023
Region / City:
N/A
Topic:
Clostridium difficile, Fecal Transplants, Antibiotics
Document Type:
Research Article
Institution:
N/A
Author:
N/A
Target Audience:
Medical professionals, Researchers, Healthcare providers
Period of Validity:
N/A
Approval Date:
N/A
Date of Changes:
N/A
Year:
2026
Region / City:
Rochester, MN, USA
Topic:
Microbiome modulation in Clostridioides difficile infection
Document Type:
Clinical trial report
Institution:
Mayo Clinic; Rebiotix Inc; BioRankings LLC
Authors:
Sahil Khanna, MBBS; Darrell S. Pardi, MD; Courtney Jones; William D. Shannon, PhD, MBA; Carlos Gonzalez, MS; Ken Blount, PhD
Target Audience:
Medical researchers, gastroenterologists, microbiologists
Study Phase:
Phase 1
Treatment:
RBX7455 orally administered live biotherapeutic
Sample Type:
Fecal samples from study participants
Outcome Measures:
Safety, efficacy, microbiome composition shifts
Time Points:
Baseline, Day 1, Week 1, Week 4, Week 8, Week 12, Week 24
Analysis Methods:
Dirichlet multinomial fitting, repeated measures analysis (DMRepeat)
Publication References:
La Rosa PS et al., PLoS One 2012; Shannon WD, BioRankings Technical Report #3
Year:
2017
Region / city:
United Kingdom
Subject:
Faecal microbiota transplant (FMT) for Clostridium difficile infection and other indications
Document type:
Clinical guidelines
Organization / institution:
British Society of Gastroenterology (BSG) and Healthcare Infection Society (HIS)
Authors:
Working group of BSG and HIS
Target audience:
Healthcare professionals, clinicians
Period of validity:
Ongoing (as of publication)
Approval date:
Not specified
Date of revisions:
Not specified
Scope:
Guidelines for the use of faecal microbiota transplant in treating Clostridium difficile infection (CDI) and other clinical conditions
Clinical management:
Recommendations for patient selection, donor selection, and FMT administration
Economic aspects:
Cost-effectiveness of FMT compared to traditional therapies for CDI
Year:
2023
Region / City:
Hertfordshire
Topic:
Healthcare, Infectious Diseases
Document Type:
Case Review Template
Organ / Institution:
NHS
Author:
Not specified
Target Audience:
Healthcare providers, General Practitioners
Period of Action:
Not specified
Approval Date:
Not specified
Modification Date:
Not specified
Chapter:
2.2.4
Manual:
Terrestrial Manual
Related Chapters:
1.1.6 Principles and methods of Validation of diagnostic assays for infectious diseases of terrestrial animals; 2.2.6 Selection and use of reference samples and panels
Standard Referenced:
ISO/IEC 17025:2005; ISO/IEC 17025:2017
Issuing Organization:
World Organisation for Animal Health (WOAH)
Subject:
Estimation of measurement uncertainty in quantitative diagnostic tests
Scope:
Diagnostic assays for infectious diseases of terrestrial animals
Test Types Mentioned:
ELISA; RT-PCR
Approach Described:
Top-down (control sample) method
Statistical Measures:
Mean ± 2 SD; Relative Standard Deviation; Coefficient of Variation
Application Context:
Cut-off (threshold) values in serology and molecular diagnostics
Year:
2026
Region / City:
Santa Maria, RS, Brazil
Topic:
Photobiological properties, antimicrobial assays
Document type:
Research Article
Organization / Institution:
Universidade Federal de Santa Maria - UFSM
Author:
Morgana Maciél Oliveira, Gabrielle Aguiar Dantas, Luiz Antônio Sodré Costa, Roberto Christ Vianna Santos, Bernardo A. Iglesias
Target Audience:
Researchers in photobiology and microbiology
Date of approval:
2026
Date of changes:
N/A
Note:
Contextual Description
Year:
2009
Region / city:
Oyster Pond, MA, USA
Subject:
Genetics, Microbiology
Document type:
Table
Organization / institution:
Not specified
Author:
Orata et al. (2015)
Target audience:
Researchers in the fields of microbiology, genetics, and epidemiology
Period of validity:
Not specified
Date of approval:
Not specified
Date of changes:
Not specified
Year:
2010
Region / City:
N/A
Subject:
Biomedical Research, Cell Biology, Molecular Biology
Document Type:
Research Methods Supplement
Author:
N/A
Target Audience:
Researchers, Scientists in Biomedical Fields
Period of Validity:
N/A
Approval Date:
N/A
Date of Changes:
N/A
Year of completion:
2023
Field:
Cancer research and tumor immunology
Research focus:
PPP2R2A deletion and expression in NSCLC and immunotherapy response
Type of document:
Supplementary materials and methods section of a scientific study
Data sources:
cBioPortal Pan-Lung cancer dataset; TCGA pan-cancer RNA-Seq data via Xena Browser; Kmplot survival database
Cell lines:
A549 cells; CMT167 PPP2R2A heterozygous knockout cells
Animal models:
CD11b-DTR mice (Jackson Laboratory, strain 006000); C57BL6 mice
Experimental techniques:
RNA sequencing; Gene Set Enrichment Analysis (GSEA); glycosylation assays with PNGase F and Endo H; adoptive Treg transfer; flow cytometry; Treg suppression assay with CellTrace Violet; ELISA
Repositories:
NCBI GEO (accession number GSE311238)
Analytes:
PD-L1, TNF-α, IFN-γ, IFN-α, IFN-β
Antibodies and reagents:
Anti-Mouse PD-L1 In Vivo Antibody (Clone 10F.9G2, Ichorbio); reagents from BioLegend, STEMCELL Technologies, eBioscience, R&D Systems, PBL Assay Science
Cited works:
Gyorffy B., 2024, Innovation (Camb); Qiu Z. et al., 2020, Cancer Research; Zhou L. et al., 2024, Journal of Clinical Investigation
Year:
2024
Region / city:
India
Topic:
Immunology, Biotechnology
Document type:
Book Chapter
Institution:
Tomo Riba Institute of Health & Medical Sciences (TRIHMS)
Author:
Ashmita Banik
Target audience:
Scientific community, Researchers in Biotechnology and Immunology
Period of validity:
N/A
Approval date:
N/A
Date of changes:
N/A
Year:
2024
Region / City:
Chennai
Subject:
Toxicology, Environmental Pollution, Microplastics
Document Type:
Research Article
Organization / Institution:
Not specified
Author:
Not specified
Target Audience:
Researchers, Environmental Scientists
Period of Validity:
Not specified
Approval Date:
Not specified
Date of Amendments:
Not specified
Procedure ID:
PSAv1.0
Version number:
1.0
Release date:
20 March 2017
Developed by:
Benoit Witkowski & Didier Ménard
Institution:
Unité d’Epidémiologie Moléculaire du Paludisme, Institut Pasteur du Cambodge
Location:
Phnom Penh, Cambodia
Document type:
Laboratory procedure
Purpose:
Evaluation of Plasmodium falciparum susceptibility to piperaquine
Assay types:
In-vitro Piperaquine Survival Assay (PSA); Ex-vivo Piperaquine Survival Assay
Target organism:
Plasmodium falciparum
Drug tested:
Piperaquine (PPQ)
Drug concentration:
200 nM
Exposure duration:
48 hours
Assessment time point:
72 hours after drug exposure
Intended users:
Appropriately-equipped laboratories performing in-vitro and ex-vivo drug susceptibility testing
Quality requirements:
Training required; participation in QA/QC proficiency scheme recommended
Referenced study:
Duru et al. 2015
Contact information:
[email protected]
Document type:
Supplementary material
Subject area:
Molecular biology and cancer research
Biological focus:
MIR22HG overexpression in cervical cancer cell lines
Cell lines:
HeLa; SiHa; C-33 A (HPV-)
Experimental methods:
Cell culture; transient transfection; RNA extraction; qRT-PCR; CCK-8 assay; Transwell migration and invasion assays
Genes referenced:
MIR22HG; GAPDH
Reagents and materials:
DMEM; MEM; FBS; Lipofectamine 2000; TRIzol; SYBR Green PCR kit; Matrigel
Data included:
Figure S1; Table S1; Table S7
Dataset description:
Annotated list of 4072 lncRNAs from HGNC database
Vendors mentioned:
Keygen Biotech (Nanjing, China); GeneChem (Shanghai, China); HyClone (Logan, UT, USA); Invitrogen (Carlsbad, CA, USA); Toyobo (Osaka, Japan); Beyotime (Shanghai, China); BD (Franklin Lakes, NJ, USA); Corning (New York, USA)