№ files_lp_3_process_7_095213
Standard operating procedure describing in-vitro and ex-vivo laboratory assays for measuring the survival rate of Plasmodium falciparum parasites following controlled exposure to piperaquine.
Procedure ID: PSAv1.0
Version number: 1.0
Release date: 20 March 2017
Developed by: Benoit Witkowski & Didier Ménard
Institution: Unité d’Epidémiologie Moléculaire du Paludisme, Institut Pasteur du Cambodge
Location: Phnom Penh, Cambodia
Document type: Laboratory procedure
Purpose: Evaluation of Plasmodium falciparum susceptibility to piperaquine
Assay types: In-vitro Piperaquine Survival Assay (PSA); Ex-vivo Piperaquine Survival Assay
Target organism: Plasmodium falciparum
Drug tested: Piperaquine (PPQ)
Drug concentration: 200 nM
Exposure duration: 48 hours
Assessment time point: 72 hours after drug exposure
Intended users: Appropriately-equipped laboratories performing in-vitro and ex-vivo drug susceptibility testing
Quality requirements: Training required; participation in QA/QC proficiency scheme recommended
Referenced study: Duru et al. 2015
Contact information: [email protected]
Price: 8 / 10 USD
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