№ files_lp_4_process_3_085418
File format: docx
Character count: 84115
File size: 1283 KB
Overview of CRISPR/Cas9 applications in human oocytes and embryos, including experimental approaches, observed genetic outcomes, and associated ethical discussions.
Year:
2020
Region / City:
Ghent, Belgium
Topic:
Human germline gene editing
Document type:
Review article
Institution:
Ghent University Hospital, Center for Medical Genetics Ghent
Authors:
Bekaert B., Boel A., Cosemans G., De Witte L., Menten B., Heindryckx B.
Corresponding author:
[email protected]
Keywords:
human germline gene editing, loss-of-heterozygosity, mosaicism, CRISPR/Cas9
Focus:
Preimplantation embryos and oocytes
Methodologies:
Knock-out and knock-in experiments, base editing, prime editing
Challenges addressed:
Mosaicism, off-target editing, loss-of-heterozygosity
Ethical considerations:
Use of non-viable embryos, evaluation of ethical implications of HGGE
Publication type:
Peer-reviewed scientific review
Price: 8 / 10 USD
The file will be delivered to the email address provided at checkout within 12 hours.
The file will be delivered to the email address provided at checkout within 12 hours.
Don’t have cryptocurrency yet?
You can still complete your purchase in a few minutes:- Buy Crypto in a trusted app (Coinbase, Kraken, Cash App or any similar service).
- In the app, tap Send.
- Select network, paste our wallet address.
- Send the exact amount shown above.
The final amount may vary slightly depending on the payment method.
The file will be sent to the email address provided at checkout within 24 hours.
The product description is provided for reference. Actual content and formatting may differ slightly.
Document type:
Supplementary material
Study focus:
Construction and validation of kduD gene mutants
Methodology:
CRISPR/Cas9 system and transposon mutagenesis
Organism:
Escherichia coli (Ec032 and derivatives)
Experimental assays:
Biofilm biomass quantification, ERIC-PCR, Sanger sequencing
Content elements:
Figures S1–S2, Tables S1–S2
Strains described:
Ec032, Ec032Δ, Ec032ΔkduD, E. coli DH5α, E. coli WM3064
Plasmids described:
pCure-oriT-GFP-MCR, pCat-arr, pCasKp-OriT, pSGKp-arr2, pSGKp-Ec032-kduD, pSGKp-Ec032-kduD-500
Genetic elements:
kduD gene, sgRNA constructs, repair arms
Antibiotic resistance markers:
Apramycin, Rifampicin, Colistin
Techniques included:
Crystal violet staining, electrophoretogram analysis, primer design
Year:
2018
Institution:
Department of Microbiology and Molecular Genetics, Oklahoma State University, USA
Field:
Biology
Document Type:
Research article
Author:
D. Jaganathan, K. Ramasamy, G. Sellamuthu, S. Jayabalan, G. Venkataraman
Keywords:
crop, genome, CRISPR/Cas9, genetic engineering, improvement
Target Audience:
Scientists, researchers in agriculture and genetics
Scope:
Crop disease resistance, abiotic stress tolerance, genome editing efficiency
Reference Systems:
TALEN, ZFN, CRISPR/Cas9
Period Covered:
1996–2018
Year:
2024
Region / City:
International
Subject:
Breast Cancer, Genetic Testing
Document Type:
Expert Consensus Report
Organization / Institution:
ESMO Precision Oncology Working Group
Author:
ESMO Precision Oncology Working Group
Target Audience:
Clinicians, Medical Oncologists, Geneticists
Period of validity:
2024
Approval Date:
Not specified
Date of Changes:
Not specified
Year:
2016-2021
Species studied:
Human, Collared flycatcher, Herring, Cattle, Chimpanzee, Mouse, Rhesus macaque, Grey mouse lemur, Cat, Marmoset
Number of trios:
13, 7, 12, 5, 1550, 150, 8, 19, 2, 11, 1
Sequencing machine:
Illumina HiSeq, Illumina HiSeq 2500, Illumina HiSeq X, Illumina HiSeq 2000, Illumina HiSeq 2000, Complete Genomics Inc.
Sequencing depth:
24.7X, 40X, 60X, 65.8X, 23X, 78X, 28.5X, 81X, 34.5X, 41X, 75X
Mapping:
BWA, BWA mem, GATK, Picard MarkDuplicates
Variant calling method:
DeNovoGear, GATK, FreeBayes, Strelka
FDR:
83.1%, 35%, 13%, 5.9%, 3%, 88%, 57.4%, 91.5%, 91%, 44%
FNR:
20%, 80%, 32%, 44%, 45%
Method:
Poisson distribution, Customized filtering, Manual curation
Software:
GATK 3.3.0, GATK 3.5, Samtools
Validation method:
Sanger sequencing, Manual curation, Simulation
Date of approval:
2016-2021
End of study:
2021
Year:
2026
Region / City:
Not specified
Topic:
Immunogenetics, Clonal evolution in leukemia
Document type:
Supplementary materials and methods
Institution:
Research laboratory, unspecified
Author:
Not specified
Target audience:
Researchers in hematology and molecular biology
Period of study:
Patient cohort sampling period not specified
Date of approval:
Not specified
Sequencing depth:
IGH-DJ library average 104,771 reads per KMT2A-rearranged sample, IGH-VJ library average 42,640 reads per KMT2A-rearranged sample
Patient cohort:
KMT2A-rearranged (n=18), KMT2A-germline (n=137)
Clonal evolution assessment:
DNJ-stem analysis with VH-DJH and VH-rep mechanisms
Sample materials:
Bone marrow, peripheral blood, bone marrow trephine
Immunophenotype:
pro-B, c-/pre-B
Age at entry:
18–56 years
White blood cell count:
specified per patient
Gene fusion:
KMT2A-MLLT1, KMT2A-AFF1, KMT2A-UBASH3B
Note:
Gene
Year:
2020
Region / city:
Not specified
Topic:
Genetic variants, Germline mutations
Document type:
Supplementary Table
Author:
Matejcic et al
Target audience:
Researchers in genetics, oncology
Period of validity:
Not specified
Approval date:
Not specified
Modification date:
Not specified
Study ID:
A211801
Study name:
BRCA-P
Document type:
Clinical trial eligibility criteria summary
Study type:
Randomized, double-blind, placebo-controlled clinical trial
Phase:
Phase 3
Geographic scope:
International multi-center study
Medical field:
Oncology
Target condition:
Breast cancer prevention in BRCA1 germline mutation carriers
Investigational drug:
Denosumab
Control:
Placebo
Population:
Women with confirmed deleterious or likely deleterious BRCA1 germline mutation aged 25–55 years
Key inclusion criteria:
No evidence of breast cancer by MRI or mammography and clinical breast examination within 6 months, no clinical evidence of ovarian cancer, ECOG performance status 0–1, written informed consent
Key exclusion criteria:
Prior bilateral mastectomy, history of breast or ovarian cancer, prior denosumab use, pregnancy or lactation, clinically relevant hypocalcaemia, certain prior hormonal or investigational treatments, significant medical or psychiatric conditions, active hepatitis B, hepatitis C, or HIV infection
Note:
en
Year:
2014
Topic:
Academic publishing, editing, and manuscript preparation
Document type:
Guidelines for authors
Organization / Institution:
IGI Global
Target audience:
Authors and editors preparing academic chapters for publication
Year:
Not specified
Region / City:
Not specified
Theme:
Wood doors specification for construction projects
Document Type:
Specification
Organization / Institution:
Simpson Door Company
Author:
Not specified
Target Audience:
Designers and builders
Period of validity:
Not specified
Approval Date:
Not specified
Date of amendments:
Not specified
Year:
2026
Organization:
IQNavigator
Audience:
Hiring Manager and Program Office staff
Document Type:
Procedure/Instruction
Scope:
Assignment lifecycle management
Topics:
Assignment edits, contract amendments, onboarding checklist, assignment closure
Effective Date:
2026-02-05
Year:
2016
Region / City:
United States
Topic:
IT Services, Veterans Affairs
Document Type:
Performance Work Statement (PWS)
Agency / Organization:
Department of Veterans Affairs, Veterans Health Administration
Author:
Not specified
Target Audience:
Contractors, Veterans Affairs personnel
Period of Effectiveness:
2016-2017
Approval Date:
May 11, 2016
Date of Changes:
Not specified
Year:
2010
Region / City:
Not specified
Subject:
Microsoft Word 2010 Editing Tools
Document Type:
Training Material
Organization:
Not specified
Author:
Not specified
Target Audience:
Adults
Duration:
90 minutes
Period of Activity:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Equipment Requirements:
Projector, computers with internet connections, laser pointer
Software Requirements:
Windows 7, Microsoft Word 2010
Material Requirements:
Pens, activity sheets, practice files, handouts, surveys
Learning Objectives:
Cut, copy, paste text, find and replace text, use thesaurus and grammar check
Assessment Method:
Completion of class activities
Agenda:
Outline class content
Note:
Context
Year:
2019
Region / City:
Purdue University
Topic:
Timekeeping Instructions
Document Type:
Cheat Sheet
Institution:
Purdue University
Author:
Purdue University
Target Audience:
Purdue Employees
Period of Validity:
N/A
Approval Date:
N/A
Date of Changes:
3/20/2019
Year:
2024
Region / City:
Toronto, Canada
Topic:
Editing, Professional Writing, Publishing
Document Type:
Syllabus, Course Outline
Organization:
York University, Faculty of Liberal Arts and Professional Studies
Author:
Dr. Matt Bucemi
Target Audience:
Students of PRWR 2006
Period of Action:
Winter 2024
Approval Date:
N/A
Revision Date:
N/A
Year:
2023
Organization:
International Telecommunication Union
Sector:
ITU Telecommunication Standardization Sector (ITU-T)
Publication type:
Informative publication
Document type:
Author’s guide
Approval body:
Telecommunication Standardization Advisory Group (TSAG)
Revision date:
June 2023
Scope:
Preparation and presentation of ITU-T Recommendations
Intended users:
ITU-T study group authors
Language:
English
Copyright holder:
International Telecommunication Union
Rights:
All rights reserved
Year:
2015
Region / City:
Raleigh, North Carolina
Theme:
Sales, Training, Business Communication
Document Type:
Letter
Organization / Institution:
Quality Furnishings
Author:
Donald Weston
Target Audience:
Mr. Roger Powell, Sales Manager
Period of Validity:
N/A
Approval Date:
N/A
Modification Date:
N/A
Note:
Year
Contextual description:
A manual outlining the usage of Adobe Photoshop, including various editing tools, file handling, and image manipulation techniques.
Note:
Year
Year:
2024
Region / city:
Global
Topic:
Mitochondrial genetics, gene editing technologies, therapeutic potential
Document type:
Scientific article
Institution:
Not specified
Author:
Gizem INAL
Target audience:
Researchers, clinicians, and medical professionals
Period of validity:
Not specified
Approval date:
Not specified
Date of changes:
Not specified