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CRISPR/Cas9 Synergistic Activation Mediator (SAM) is a tool used for gene activation screening based on a pooled human library of sgRNAs targeting human RefSeq isoforms.
Note:
Year
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Document type:
Supplementary material
Study focus:
Construction and validation of kduD gene mutants
Methodology:
CRISPR/Cas9 system and transposon mutagenesis
Organism:
Escherichia coli (Ec032 and derivatives)
Experimental assays:
Biofilm biomass quantification, ERIC-PCR, Sanger sequencing
Content elements:
Figures S1–S2, Tables S1–S2
Strains described:
Ec032, Ec032Δ, Ec032ΔkduD, E. coli DH5α, E. coli WM3064
Plasmids described:
pCure-oriT-GFP-MCR, pCat-arr, pCasKp-OriT, pSGKp-arr2, pSGKp-Ec032-kduD, pSGKp-Ec032-kduD-500
Genetic elements:
kduD gene, sgRNA constructs, repair arms
Antibiotic resistance markers:
Apramycin, Rifampicin, Colistin
Techniques included:
Crystal violet staining, electrophoretogram analysis, primer design
Year:
2018
Institution:
Department of Microbiology and Molecular Genetics, Oklahoma State University, USA
Field:
Biology
Document Type:
Research article
Author:
D. Jaganathan, K. Ramasamy, G. Sellamuthu, S. Jayabalan, G. Venkataraman
Keywords:
crop, genome, CRISPR/Cas9, genetic engineering, improvement
Target Audience:
Scientists, researchers in agriculture and genetics
Scope:
Crop disease resistance, abiotic stress tolerance, genome editing efficiency
Reference Systems:
TALEN, ZFN, CRISPR/Cas9
Period Covered:
1996–2018
Year:
2020
Region / City:
Ghent, Belgium
Topic:
Human germline gene editing
Document type:
Review article
Institution:
Ghent University Hospital, Center for Medical Genetics Ghent
Authors:
Bekaert B., Boel A., Cosemans G., De Witte L., Menten B., Heindryckx B.
Corresponding author:
[email protected]
Keywords:
human germline gene editing, loss-of-heterozygosity, mosaicism, CRISPR/Cas9
Focus:
Preimplantation embryos and oocytes
Methodologies:
Knock-out and knock-in experiments, base editing, prime editing
Challenges addressed:
Mosaicism, off-target editing, loss-of-heterozygosity
Ethical considerations:
Use of non-viable embryos, evaluation of ethical implications of HGGE
Publication type:
Peer-reviewed scientific review
Note:
Year
Hourly Rate:
$200.00
Available Counties:
All
Registered:
General Civil and Domestic
Year:
2025
Region / City:
Gauteng, South Africa
Subject:
Mediation agreement between plaintiff, defendant, and mediator
Document Type:
Legal Agreement
Organization / Institution:
High Court of South Africa, Gauteng Division
Author:
N/A
Target Audience:
Parties involved in legal disputes
Effective Period:
Until the mediation process concludes
Date of Approval:
N/A
Date of Amendments:
N/A
Jurisdiction:
New South Wales, Australia
System:
National Mediator Accreditation System (NMAS)
Accreditation Body:
Alternative Dispute Resolution Directorate (Recognised Mediator Accreditation Body under the Mediator Standards Board)
Administering Authority:
NSW Department of Justice
Document Type:
Application Form for Re-Accreditation
Purpose:
Renewal of mediator accreditation under NMAS
Applicable Standards:
National Mediator Accreditation Standards
Relevant Legislation and Instruments:
NSW Department of Justice Code of Conduct; applicable CJC legislation, guidelines, practice directions and policies
Minimum Practice Requirement:
25 hours of mediation within a two-year cycle (or minimum 10 hours with supplementary requirements under specified circumstances)
Submission Requirement:
Application to be submitted at least 6 weeks prior to expiry of current accreditation
Intended Applicants:
Accredited mediators seeking renewal of NMAS accreditation
Contact:
[email protected]
Note:
; Phone 02 8688 7455
Year:
2026
Region / City:
Iowa, United States
Document Type:
Court Form
Court:
Iowa District Court
Parties:
Petitioner and Respondent (unspecified)
Purpose:
Request for reduced mediation fees
Information Included:
Personal, financial, and household details
Signature Required:
Yes
Date of Submission:
Not specified
Year:
2026
Location:
Gauteng, South Africa
Type of document:
Legal agreement
Subject:
Dispute resolution through mediation
Parties involved:
Plaintiff, Defendant
Mediator:
Johannes Gysbertus Bergenthuin SC
Legal framework:
Mediation Protocol Applicable in the Gauteng Division of the High Court
Fee structure:
R3,500 per hour or R35,000 per day, ex VAT
Confidentiality:
Mandatory for all participants
Authority:
Representatives signing on behalf of parties
Termination conditions:
Withdrawal, impasse, or written settlement
Reporting:
Mediator Report and Joint Minute to be filed with the High Court
Year:
2026
Region / City:
Ontario, Canada
Subject:
Mediation Accreditation
Document Type:
Application Form
Organization:
ADR Institute of Ontario
Author:
ADR Institute of Ontario
Target Audience:
Mediators seeking Q.Med designation
Required Training Hours:
80 hours
Mandatory Courses:
ADRIO Ethics, Basic Mediation Training, Specialized Mediation Training
Experience Requirement:
Completion of two mediations (sole or co-mediation)
Continuing Education Requirement:
60 points every three years
Filing Fee:
Required
Submission Format:
PDF via email
Year:
2026
Region / City:
Not specified
Subject:
Lipid mediators in epilepsy
Document type:
Supplementary tables and figures
Institution:
Not specified
Authors:
Not specified
Study population:
Patients with chronic epilepsy (n=8) and controls (n=6)
Methods:
LC-MS-MS analysis of plasma and cerebrospinal fluid
Analytes:
DHA, EPA, AA, resolvins, hydroxy-fatty acids, prostaglandins, lipoxins, leukotrienes
Variables analyzed:
Gender, seizure types, number of antiseizure medicines, age
Statistical analysis:
P values, multiple linear regression
Scale:
National Hospital Seizure Severity Scale (NHS3)
Correlation measures:
Plasma and CSF levels of RvD1 and RvE1 with clinical parameters
Year:
2026
Organization:
Family Mediation Council (FMC)
Document Type:
Registration Form
Target Audience:
Family mediators seeking FMC accreditation
Required Training:
Family Mediation Foundation Training
Payment Method:
BACS transfer
Sections Included:
Personal Information, Practice Information, Contact Details, Good Standing Declaration, Final Declaration
Purpose:
Registration for accreditation and public listing of mediators
Year:
2024
Region / City:
USA
Topic:
Synergistic Activities for NSF Senior Personnel
Document Type:
Template
Organization:
Georgia Tech Office of Research Development and Office of Sponsored Programs
Target Audience:
Senior/Key Personnel applying for NSF funding
Period of Validity:
Effective May 20, 2024
Approval Date:
Not specified
Modification Date:
Not specified
Year:
2024
Region / City:
Georgia Tech, USA
Topic:
Synergistic Activities, NSF PAPPG
Document Type:
Template
Organization:
Georgia Tech Office of Research Development, Office of Sponsored Programs
Author:
Georgia Tech Office of Research Development and Office of Sponsored Programs
Target Audience:
Senior/Key Personnel submitting proposals to NSF
Effective Date:
May 20, 2024
Changes Date:
Not specified
Year:
2023
Region / city:
Tamil Nadu, Coimbatore
Topic:
Catalytic co-pyrolysis, Waste valorization, Biofuel production
Document type:
Research article
Organization / institution:
RVS Technical Campus-Coimbatore, Karpagam College of Engineering
Authors:
T.Stephen Livingston, A. Anci Manon Mary
Target audience:
Researchers, Environmental scientists, Engineers
Period of validity:
N/A
Approval date:
N/A
Date of amendments:
N/A
Year:
2023
Region / City:
Subarnarekha River Basin, Eastern India
Theme:
Climate Change, Environmental Stressors, Freshwater Ecology
Document Type:
Review Article
Organization / Institution:
N/A
Author:
N/A
Target Audience:
Researchers, Environmental Scientists, Ecologists
Period of Validity:
N/A
Date of Approval:
N/A
Date of Changes:
N/A
Author:
Dr. Igor Shesho
Academic Title:
Full Professor
Thematic Area:
District heating, renewable energy, urban energy systems
Type of Document:
Scientific research paper
Geographical Focus:
City of Ohrid, R. Macedonia
Subject:
Techno-economic assessment of district heating systems with renewable energy integration
Methodology:
Development and analysis of five simulation scenarios
Key Indicators:
MWh/km, Levelized Cost of Heat (LCOH), Levelized Cost of Energy (LCOE)
Energy Sources Considered:
Solar thermal, heat pumps, seasonal heat storage, photovoltaic systems, combined heat and power (CHP)
Building Types Analyzed:
Residential buildings, schools, offices, hospitals, administrative facilities
Scope:
Existing urban buildings with varying energy performance classes
Primary Focus:
Economic feasibility and environmental impact of district heating investments
Year:
2026
Region / City:
Nanjing, China
Topic:
Environmental science, Soil health
Document type:
Research article
Author:
Wenjun Xie, Zijian Qiu, Dandan Li, Zhouzhang Wang, Nan Gao, Ruonan Xiong, Adharsh Rajasekar, Xinhua He, Weishou Shen
Target audience:
Researchers in environmental science, soil health, and agriculture
Period of validity:
N/A
Date of approval:
N/A
Date of modification:
N/A
Keywords:
Pseudomonas stutzeri, organic fertilizers, N2O emission, microbial community, greenhouse, vegetable fields
Context:
This research article examines the combined impact of Pseudomonas stutzeri NRCB010 and organic fertilizers on reducing N2O emissions and altering microbial communities in greenhouse vegetable cultivation.
Year:
2026
Region / City:
Port Harcourt
Topic:
Bioactive compounds, plant protection
Document Type:
Research Paper
Author:
Unknown
Target Audience:
Scientists, Researchers, Botanists
Period of Action:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Year:
2026
Region / City:
Baghdad, Iraq
Topic:
Type 2 Diabetes Mellitus, Periodontal Disease, Fungal Infections, Obesity, Inflammation
Document Type:
Research Article
Institution:
Gilgamesh University, College of Health and Medical Techniques
Authors:
Mohammed Mohaibes, Ammar Sabar, Mayada Al-khafaji
Target Audience:
Medical researchers, clinicians, and healthcare professionals
Study Design:
Case-control study
Sample Size:
150 participants (75 T2DM patients, 75 healthy controls)
Methods:
Oral swabs, blood analysis for CRP and ESR, fungal isolation via microscopy, culture, VITEK® 2 system
Period:
Not explicitly stated
Publication Status:
Preprint / Manuscript
Keywords:
Type 2 Diabetes Mellitus, Periodontal disease, Fungal infection, Obesity, Inflammation, CRP, ESR
Correspondence:
[email protected]