№ lp_2_3_18330
File format: docx Character count: 3390 File size: 247 KB
Supplementary scientific dataset presenting experimental validation, genetic constructs, bacterial strains, plasmids, and primer sequences associated with CRISPR/Cas9-mediated deletion of the kduD gene in Escherichia coli.
Document type: Supplementary material
Study focus: Construction and validation of kduD gene mutants
Methodology: CRISPR/Cas9 system and transposon mutagenesis
Organism: Escherichia coli (Ec032 and derivatives)
Experimental assays: Biofilm biomass quantification, ERIC-PCR, Sanger sequencing
Content elements: Figures S1–S2, Tables S1–S2
Strains described: Ec032, Ec032Δ, Ec032ΔkduD, E. coli DH5α, E. coli WM3064
Plasmids described: pCure-oriT-GFP-MCR, pCat-arr, pCasKp-OriT, pSGKp-arr2, pSGKp-Ec032-kduD, pSGKp-Ec032-kduD-500
Genetic elements: kduD gene, sgRNA constructs, repair arms
Antibiotic resistance markers: Apramycin, Rifampicin, Colistin
Techniques included: Crystal violet staining, electrophoretogram analysis, primer design
Price: 8 / 10 USD
The file will be delivered to the email address provided at checkout within 12 hours.

Don’t have cryptocurrency yet?

You can still complete your purchase in a few minutes:
  1. Buy Crypto in a trusted app (Coinbase, Kraken, Cash App or any similar service).
  2. In the app, tap Send.
  3. Select network, paste our wallet address.
  4. Send the exact amount shown above.
After sending, paste your TXID (transaction ID) and your email to receive the download link. Need help? Contact support and we’ll guide you step by step.

The final amount may vary slightly depending on the payment method.
The file will be sent to the email address provided at checkout within 24 hours.
The product description is provided for reference. Actual content and formatting may differ slightly.
CRISPR/Cas9 Synergistic Activation Mediator (SAM) pooled human library
Note: Year
Crop improvement using the CRISPR/Cas9 system
Year: 2018
Institution: Department of Microbiology and Molecular Genetics, Oklahoma State University, USA
Field: Biology
Document Type: Research article
Author: D. Jaganathan, K. Ramasamy, G. Sellamuthu, S. Jayabalan, G. Venkataraman
Keywords: crop, genome, CRISPR/Cas9, genetic engineering, improvement
Target Audience: Scientists, researchers in agriculture and genetics
Scope: Crop disease resistance, abiotic stress tolerance, genome editing efficiency
Reference Systems: TALEN, ZFN, CRISPR/Cas9
Period Covered: 1996–2018
CRISPR/Cas9 gene editing in the human germline: current progress and challenges
Year: 2020
Region / City: Ghent, Belgium
Topic: Human germline gene editing
Document type: Review article
Institution: Ghent University Hospital, Center for Medical Genetics Ghent
Authors: Bekaert B., Boel A., Cosemans G., De Witte L., Menten B., Heindryckx B.
Corresponding author: [email protected]
Keywords: human germline gene editing, loss-of-heterozygosity, mosaicism, CRISPR/Cas9
Focus: Preimplantation embryos and oocytes
Methodologies: Knock-out and knock-in experiments, base editing, prime editing
Challenges addressed: Mosaicism, off-target editing, loss-of-heterozygosity
Ethical considerations: Use of non-viable embryos, evaluation of ethical implications of HGGE
Publication type: Peer-reviewed scientific review
Supplementary Figure S1. Amivantamab shows antitumor effect and suppresses the expression of EGFR Exon20ins mutant in Ba/F3 cells overexpressing the indicated EGFR Exon20ins mutations
Year: 2023
Region / city: Not specified
Topic: Cancer research, EGFR mutations
Document type: Research figure
Organization / institution: Not specified
Author: Not specified
Target audience: Researchers, oncologists
Effective period: Not specified
Approval date: Not specified
Modification date: Not specified
Genetic Variability and Yellow Vein Mosaic Virus Resistance in Mutant Lines of Blackgram (Vigna mungo L. Hepper) under Field Conditions
Year: 2022–2023
Region / City: Bavikere, Shivamogga, Karnataka, India
Crop: Blackgram (Vigna mungo L.)
Type of Document: Research article
Institution: Agricultural and Horticultural Research Station (AHRS)
Experimental Material: 90 F4M3 lines from PU31 × Rashmi cross, 30 selected F5M4 lines
Methods: Field experiment, augmented and RCBD designs, gamma irradiation treatment (20 Kr)
Target Trait: Yellow Vein Mosaic Virus (YVMV) resistance
Key Measurements: Seed yield, disease incidence, disease index, PCV, GCV, heritability, genetic advance
Significant Lines: BLM 9, BLM 20, BLM 29, BLM 44, BLM 51, BLM 58 (first season); BLM 30, BLM 51 (second season)
Segregation level of the mutant strains and growth curve analysis of FtsZ mutants in Anabaena sp.
Note: Year
Theme: Cell division, Mutant strains, Fluorescent proteins
Document Type: Scientific Supplementary Information
Target Audience: Researchers, Molecular Biologists
Genetic Variability and Yellow Vein Mosaic Virus (YVMV) Resistance in Mutant Lines of Blackgram(Vigna mungo L. Hepper) under Field Condition
Year: 2022
Region / City: Shivamogga, Karnataka, India
Theme: Genetic variability, crop resistance, blackgram breeding
Document Type: Research Paper
Organization: Agricultural and Horticultural Research Station (AHRS), Bavikere
Author: Not specified
Target Audience: Researchers, agronomists, plant breeders
Period of Activity: Kharif 2022, Summer 2023
Date of Approval: Not specified
Date of Modifications: Not specified
Which component of lipopolysaccharide is essential for maintaining outer membrane stability in Gram-negative bacteria and would be absent in a mutant unable to synthesize KDO?
Year: Not specified
Region / City: Not specified
Topic: Bacterial biology, Gram-negative bacteria, Lipopolysaccharides
Document Type: Educational Material
Organization / Institution: Not specified
Author: Not specified
Target Audience: Students or professionals in microbiology
Period of Validity: Not specified
Approval Date: Not specified
Date of Changes: Not specified
Please provide the following information required for genetic analysis of your mutant mice.
Note: Year
ESR1 F404 mutations and fulvestrant resistance in ESR1 mutant breast cancer
Year: 2026
Region / City: London, United Kingdom; Urbana, Illinois, USA; New York City, USA; Glasgow, United Kingdom
Topic: Breast cancer, ESR1 mutations, drug resistance
Document Type: Research article
Institution: The Institute of Cancer Research; University of Illinois at Urbana-Champaign; Memorial Sloan Kettering Cancer Center; Weill Cornell Medical College; The Royal Marsden Hospital
Authors: Belinda Kingston, Alex Pearson, Maria Teresa Herrera-Abreu, Li-Xuan Sim, Rosalind J Cutts, Heena Shah, Laura Moretti, Lucy S Kilburn, Hannah Johnson, Iain R Macpherson, Alistair Ring, Judith M Bliss, Yingwei Hou, Weiyi Toy, John A Katzenellenbogen, Sarat Chandarlapaty, Nicholas C Turner
Keywords: Fulvestrant, acquired resistance, ESR1 F404, breast cancer, PIK3CA, TP53, ESR1 D538G, ESR1 E380Q, ESR1 Y537N
Methods: Patient cohort analysis, progression-free survival assessment, CRISPR gene editing, RNA sequencing, clonogenic assays
Target Audience: Oncologists, cancer researchers, molecular biologists
Supplementary Data: Figures 1–9, mutation incidence, gene expression, drug response curves
Clinical Focus: Acquired resistance to fulvestrant in ESR1 mutant breast cancer models
Field Validation of Salt Tolerance in Gamma- Ray-induced Potato Mutant Line Selection In Vitro under Saline Soil Condition
Year: 2021
Region / City: Iraq
Topic: Salt Tolerance, Potato Mutation Breeding
Document Type: Research Article
Organization: Scientific Research Commission, Baghdad, Iraq
Author: AL-Hussaini Z.A., Abdulsada A.J., AL-Ani M.A.
Target Audience: Researchers, Agronomists
Period of Validity: N/A
Approval Date: N/A
Modification Date: N/A
Table S2: D201 mutant activities
Year: 2021
Region / city: N/A
Topic: Mutant codon activities
Document type: Research table
Organization / institution: N/A
Author: N/A
Target audience: Researchers in genetics and molecular biology
Effective period: N/A
Approval date: N/A
Date of amendments: N/A
Topics: kduD CRISPRCas9 mutant