№ files_lp_4_process_1_42642
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This document provides instructions for submitting genetic analysis information for mutant mice, including protocols for PCR, reagent preparation, and electrophoresis.
Note:
Year
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Year:
2023
Region / city:
Not specified
Topic:
Cancer research, EGFR mutations
Document type:
Research figure
Organization / institution:
Not specified
Author:
Not specified
Target audience:
Researchers, oncologists
Effective period:
Not specified
Approval date:
Not specified
Modification date:
Not specified
Year:
2022–2023
Region / City:
Bavikere, Shivamogga, Karnataka, India
Crop:
Blackgram (Vigna mungo L.)
Type of Document:
Research article
Institution:
Agricultural and Horticultural Research Station (AHRS)
Experimental Material:
90 F4M3 lines from PU31 × Rashmi cross, 30 selected F5M4 lines
Methods:
Field experiment, augmented and RCBD designs, gamma irradiation treatment (20 Kr)
Target Trait:
Yellow Vein Mosaic Virus (YVMV) resistance
Key Measurements:
Seed yield, disease incidence, disease index, PCV, GCV, heritability, genetic advance
Significant Lines:
BLM 9, BLM 20, BLM 29, BLM 44, BLM 51, BLM 58 (first season); BLM 30, BLM 51 (second season)
Document type:
Supplementary material
Study focus:
Construction and validation of kduD gene mutants
Methodology:
CRISPR/Cas9 system and transposon mutagenesis
Organism:
Escherichia coli (Ec032 and derivatives)
Experimental assays:
Biofilm biomass quantification, ERIC-PCR, Sanger sequencing
Content elements:
Figures S1–S2, Tables S1–S2
Strains described:
Ec032, Ec032Δ, Ec032ΔkduD, E. coli DH5α, E. coli WM3064
Plasmids described:
pCure-oriT-GFP-MCR, pCat-arr, pCasKp-OriT, pSGKp-arr2, pSGKp-Ec032-kduD, pSGKp-Ec032-kduD-500
Genetic elements:
kduD gene, sgRNA constructs, repair arms
Antibiotic resistance markers:
Apramycin, Rifampicin, Colistin
Techniques included:
Crystal violet staining, electrophoretogram analysis, primer design
Note:
Year
Theme:
Cell division, Mutant strains, Fluorescent proteins
Document Type:
Scientific Supplementary Information
Target Audience:
Researchers, Molecular Biologists
Year:
2022
Region / City:
Shivamogga, Karnataka, India
Theme:
Genetic variability, crop resistance, blackgram breeding
Document Type:
Research Paper
Organization:
Agricultural and Horticultural Research Station (AHRS), Bavikere
Author:
Not specified
Target Audience:
Researchers, agronomists, plant breeders
Period of Activity:
Kharif 2022, Summer 2023
Date of Approval:
Not specified
Date of Modifications:
Not specified
Year:
Not specified
Region / City:
Not specified
Topic:
Bacterial biology, Gram-negative bacteria, Lipopolysaccharides
Document Type:
Educational Material
Organization / Institution:
Not specified
Author:
Not specified
Target Audience:
Students or professionals in microbiology
Period of Validity:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Year:
2026
Region / City:
London, United Kingdom; Urbana, Illinois, USA; New York City, USA; Glasgow, United Kingdom
Topic:
Breast cancer, ESR1 mutations, drug resistance
Document Type:
Research article
Institution:
The Institute of Cancer Research; University of Illinois at Urbana-Champaign; Memorial Sloan Kettering Cancer Center; Weill Cornell Medical College; The Royal Marsden Hospital
Authors:
Belinda Kingston, Alex Pearson, Maria Teresa Herrera-Abreu, Li-Xuan Sim, Rosalind J Cutts, Heena Shah, Laura Moretti, Lucy S Kilburn, Hannah Johnson, Iain R Macpherson, Alistair Ring, Judith M Bliss, Yingwei Hou, Weiyi Toy, John A Katzenellenbogen, Sarat Chandarlapaty, Nicholas C Turner
Keywords:
Fulvestrant, acquired resistance, ESR1 F404, breast cancer, PIK3CA, TP53, ESR1 D538G, ESR1 E380Q, ESR1 Y537N
Methods:
Patient cohort analysis, progression-free survival assessment, CRISPR gene editing, RNA sequencing, clonogenic assays
Target Audience:
Oncologists, cancer researchers, molecular biologists
Supplementary Data:
Figures 1–9, mutation incidence, gene expression, drug response curves
Clinical Focus:
Acquired resistance to fulvestrant in ESR1 mutant breast cancer models
Year:
2021
Region / City:
Iraq
Topic:
Salt Tolerance, Potato Mutation Breeding
Document Type:
Research Article
Organization:
Scientific Research Commission, Baghdad, Iraq
Author:
AL-Hussaini Z.A., Abdulsada A.J., AL-Ani M.A.
Target Audience:
Researchers, Agronomists
Period of Validity:
N/A
Approval Date:
N/A
Modification Date:
N/A
Year:
2021
Region / city:
N/A
Topic:
Mutant codon activities
Document type:
Research table
Organization / institution:
N/A
Author:
N/A
Target audience:
Researchers in genetics and molecular biology
Effective period:
N/A
Approval date:
N/A
Date of amendments:
N/A
Year:
2023
Region / City:
Bloomington, Indiana
Topic:
Animal Welfare, Veterinary Medicine, Anesthesia
Document Type:
Guidelines
Author:
Indiana University Bloomington Laboratory Animal Resources veterinary staff
Target Audience:
Laboratory staff, researchers, veterinarians
Period of Validity:
N/A
Approval Date:
N/A
Date of Last Revision:
N/A
Document type:
Materials and Methods section
Species:
C57Bl6/J mice; Sprague Dawley rats
Animal source:
The Jackson Laboratories (Bar Harbor, Maine); Zhejiang Vital River Laboratory Animal Technology Co., Ltd.
Experimental facilities:
BioDuro-Sundia (Shanghai, China)
Ethics approval:
IACUC approved protocols
Regulatory compliance:
Animal Welfare Act (9 CFR Parts 1–3); Guide for the Care and Use of Laboratory Animals (8th Edition); AAALAC accreditation (001516)
Cell lines:
YPMK; YPMK PINK1 KO; EPF1; HEK293 PINK1 KO; SK-OV-3; ΔOTC
Primary cultures:
Mouse hippocampal and cortical neurons (E16, A53T α-synuclein M83 mice)
Key assay:
SMCxPRO pUb assay
Key reagents:
Rabbit anti-pS65-Ub antibody (CST E2J6T); Mouse anti-Ub antibody (CST P4D1); K48 pUb tetramers (RnD Systems UC-250)
Instrumentation:
SMCxPRO system; Agilent Seahorse XFe96 analyzer; ImageXpress Micro Confocal High-Content Imaging System
Experimental focus:
α-synuclein preformed fibrils (PFFs); mitochondrial respiration; mitophagy measurement (MT-mKeima)
Data availability:
Available from corresponding author upon reasonable request under Material Transfer Agreement
Code availability:
No original code reported
Year:
2023
Region / city:
Bristol, UK
Field:
Immunology, Autoimmune Diseases
Document Type:
Research article
Institution:
University of Bristol
Author:
Not specified
Target Audience:
Researchers in immunology, medical professionals
Period of validity:
Not specified
Approval Date:
Not specified
Date of amendments:
Not specified
Year:
1937
Region / City:
California, USA
Topic:
Literature, Novel Analysis
Document Type:
Literary Analysis
Organization / Institution:
N/A
Author:
N/A
Target Audience:
Students, Educators, Literary Scholars
Period of Validity:
N/A
Approval Date:
N/A
Date of Changes:
N/A
Year:
2023
Region / city:
Kawasaki, Tokyo, Hamamatsu, Japan
Subject:
Biomedical research, gene editing, transgenic mice
Document type:
Research appendix
Organization / institution:
In-Vivo Science Inc., CLEA Japan, Japan SLC Inc., Thermo Fisher Scientific
Author:
Not specified
Target audience:
Researchers, scientists in the field of molecular biology and genetics
Effective period:
Not specified
Approval date:
Not specified
Modification date:
Not specified
Year:
2023
Region / City:
Xi’an, Lanzhou, Wuhan
Subject:
Pharmaceutical research
Document type:
Research article
Authors:
Qingyue Da, Min Xu, Yiting Tian, Huiping Ma, Haibo Wang, Linlin Jing
Target audience:
Researchers in pharmaceutical sciences, medical professionals
Period of validity:
N/A
Approval date:
N/A
Amendment date:
N/A
Year:
2026
Region / city:
Not specified
Topic:
Toxicity, drug efficacy
Document type:
Scientific figure
Organization / institution:
Not specified
Author:
Not specified
Target audience:
Researchers, scientists
Period of validity:
Not specified
Approval date:
Not specified
Date of changes:
Not specified
Year:
2023
Region / City:
England, Northern Ireland
Topic:
Mental Health, Epilepsy, Psychological Therapy
Document Type:
Clinical Trial Report
Organization:
UCL Great Ormond Street Institute of Child Health
Author:
Sophie D. Bennett, J. Helen Cross, Kashfia Chowdhury, Tamsin Ford, Isobel Heyman, Anna E. Coughtrey, Emma Dalrymple, Sarah Byford, Bruce Chorpita, Peter Fonagy, Rona Moss-Morris, Colin Reilly, Jonathan A. Smith, Terence Stephenson, Sophia Varadkar, James Blackstone, Harriet Quartly, Tyler Hughes, Amy Lewins, Elana Moore, Fahreen Walji, Alice Welch, Emily Whelan, Alice Zacharia, Anais D’Oelsnitz, Mariam Shah, Laila Xu, Aikaterini Vezyroglou, Kirsten Mitchell, Isabella E. Nizza, Poushali Ganguli, Roz Shafran
Target Audience:
Researchers, Clinicians, Policy Makers, Families
Period of Action:
August 2019 – February 2022
Approval Date:
Not specified
Date of Changes:
Not specified
Summary:
Randomised controlled trial assessing the effectiveness of the Mental Health Intervention for Children with Epilepsy (MICE) in treating mental health difficulties in children and young people with epilepsy, alongside usual care.
Year:
1937
Region / City:
California
Theme:
Isolation, Social Injustice, Prejudice, Violence
Type of Document:
Literary Analysis
Author:
Unknown
Target Audience:
Literary Scholars, Students, Readers of American Literature
Period of Validity:
Not specified
Approval Date:
Not specified
Date of Modifications:
Not specified
Year:
1930s
Region / City:
United States
Theme:
Social Issues, Gender, Racism, The American Dream
Document Type:
Excerpt from a Novel
Organization / Institution:
N/A
Author:
John Steinbeck
Target Audience:
General readers, students of literature
Period of Validity:
N/A
Approval Date:
N/A
Date of Changes:
N/A