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This document provides a protocol for performing immunofluorescence assays on HeLa cells.
Year:
2023
Region / city:
Parkville, Victoria, Australia
Topic:
Immunofluorescence, laboratory techniques
Document type:
Laboratory protocol
Organization:
Walter and Eliza Hall Institute of Medical Research
Author:
Thanh Ngoc Nguyen
Target audience:
Laboratory researchers, biotechnologists
Date of approval:
Not specified
Date of changes:
Not specified
Price: 8 / 10 USD
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The product description is provided for reference. Actual content and formatting may differ slightly.
Year:
Not specified
Region / City:
Not specified
Topic:
Cancer research, Urothelial carcinoma
Document type:
Research methodology
Institution:
Not specified
Author:
Not specified
Target audience:
Researchers in oncology and immunology
Period of validity:
Not specified
Approval date:
Not specified
Modification date:
Not specified
Document type:
Supplementary methods section
Scientific field:
Molecular medicine and tumor immunology
Techniques described:
Immunofluorescence histology, multiplex antibody staining, next-generation sequencing
Biological material:
Tumor biopsy FFPE tissue sections
Immune markers analyzed:
CD8+, CD4+, Granzyme B
Sequencing platforms:
Illumina NovaSeq 6000
Bioinformatics tools:
trim_galore, STAR, featureCounts, DESeq2, GATK HaplotypeCaller, MuTect, Vardict
Genome reference:
Human genome hg38 with appended ONCOS-102 genome
Imaging equipment:
Panoramic 250 FLASH whole-slide scanner
Institutions mentioned:
Institute for Molecular Medicine Helsinki; Personalis Inc., California, USA
Sample preparation:
3.5 μm FFPE tissue sections
Staining reagents:
TSA 647, TSA 750, DAPI nuclear stain
Analysis focus:
Detection and quantification of immune cell infiltrates and differential gene expression in tumor biopsies
Year:
2026
Region / City:
Osun State, Nigeria
Topic:
Indigenous Knowledge Management
Document Type:
Research Paper
Institution:
Unspecified
Author:
Unspecified
Target Audience:
Researchers, Scholars, Cultural Practitioners
Period of Application:
Ongoing
Approval Date:
Unspecified
Date of Changes:
Unspecified
Version:
0.7
Author:
Raquel Morera – Date:01 December 2018
Last updated by:
Laurent Vreck – Date:28 March 2019
Year:
2018
Region / City:
Europe
Topic:
Network Functions Virtualisation (NFV)
Document Type:
Terms of Reference
Organization:
ETSI
Note:
Author
Raquel Morera
Target Audience:
ETSI members, Industry stakeholders
Validity Period:
April 2019 to February 2020
Approval Date:
30 January 2019
Update Date:
28 March 2019
Note:
Year
Region / City:
East Midlands
Topic:
IFA Pilot Program
Document Type:
Guidance Document
Organization / Institution:
East Midlands Strategic Migration Partnership (EM SMP)
Target Audience:
IFA providers, Local Authorities
Effective Period:
9 months
Organization:
Irish Football Association (IFA)
Committee:
IFA Disciplinary Committee
Document Type:
Official disciplinary procedure form
Form Identifier:
DCC1
Subject:
Notice of Complaint response and disciplinary challenge submission
Related Regulation:
IFA Disciplinary Code 2023/24
Users:
Club disciplinary officers, clubs, and individuals issued with a Notice of Complaint
Submission Method:
Comet system
Required Information:
Match details, alleged breach, response to complaint, sanction acceptance or refusal, supporting evidence, witnesses, and hearing attendees
Evidence Submission:
Evidence must be emailed to the Disciplinary Committee with the form
Declaration Requirement:
Signed certification confirming accuracy of submitted information
Associated Proceedings:
Disciplinary Committee review and potential hearing
Year:
2020
Region / City:
Berlin
Topic:
Technology, Innovation, Startups
Document Type:
Application Form
Organization / Institution:
IFA NEXT
Author:
IFA NEXT
Target Audience:
Companies, Startups
Period of Validity:
2020
Approval Date:
N/A
Date of Changes:
N/A
Year:
2026
Organization:
International Fertilizer Association (IFA)
Award:
Green Leaf Award
Focus Area:
Safety, Health, Environment (SHE) in Fertilizer Production
Submission Deadline:
15 February 2026
Contact Person:
Alexandra Dorison
Document Type:
Application Guidelines
Intended Participants:
Production complexes / units in the fertilizer industry
Submission Requirements:
Business profile, case study, supporting evidence
Year:
2023
Region / City:
-
Subject:
Immunology, Diagnostic Assay
Document Type:
Technical Manual
Organization:
-
Author:
-
Target Audience:
Laboratory Professionals
Period of Validity:
-
Approval Date:
-
Modification Date:
-
Year:
2025
Note:
Region / City
Theme:
Seed amplification assay innovation
Document Type:
Application Instructions
Target Audience:
Researchers in scientific and clinical fields
Effective Period:
Spring 2025
Year:
2023
Region / city:
Global
Topic:
Cell proliferation assay
Document type:
Kit instruction manual
Organization:
Abcam
Author:
Abcam Technical Team
Target audience:
Researchers, lab technicians
Period of validity:
1 year from receipt
Approval date:
2023
Date of modifications:
None
Storage conditions:
Store at -20°C in the dark
Components:
MTT Reagent, MTT Solvent
Required materials (not supplied):
Microplate reader, pipettes, 96 well plate
Safety notes:
Wear gloves, lab coat, and protective eyewear; handle reagents with care
Assay procedure:
Detailed protocol for measuring cell proliferation using MTT reagent
Interferences:
Serum or phenol red in culture medium may cause background interference
FAQ:
Instructions for background control, handling of MTT reagent, and troubleshooting
Contextual description:
Instruction manual for a research kit used to measure cell proliferation in cultured cells.
Document type:
Materials and Methods section
Species:
C57Bl6/J mice; Sprague Dawley rats
Animal source:
The Jackson Laboratories (Bar Harbor, Maine); Zhejiang Vital River Laboratory Animal Technology Co., Ltd.
Experimental facilities:
BioDuro-Sundia (Shanghai, China)
Ethics approval:
IACUC approved protocols
Regulatory compliance:
Animal Welfare Act (9 CFR Parts 1–3); Guide for the Care and Use of Laboratory Animals (8th Edition); AAALAC accreditation (001516)
Cell lines:
YPMK; YPMK PINK1 KO; EPF1; HEK293 PINK1 KO; SK-OV-3; ΔOTC
Primary cultures:
Mouse hippocampal and cortical neurons (E16, A53T α-synuclein M83 mice)
Key assay:
SMCxPRO pUb assay
Key reagents:
Rabbit anti-pS65-Ub antibody (CST E2J6T); Mouse anti-Ub antibody (CST P4D1); K48 pUb tetramers (RnD Systems UC-250)
Instrumentation:
SMCxPRO system; Agilent Seahorse XFe96 analyzer; ImageXpress Micro Confocal High-Content Imaging System
Experimental focus:
α-synuclein preformed fibrils (PFFs); mitochondrial respiration; mitophagy measurement (MT-mKeima)
Data availability:
Available from corresponding author upon reasonable request under Material Transfer Agreement
Code availability:
No original code reported
Note:
Year
Subject:
Pharmacology
Document type:
Research methodology
Target audience:
Researchers, Pharmacologists
Description:
This document provides the experimental setup, assay parameters, and performance data for the analysis of venetoclax and voriconazole in a pharmacokinetic study.
Year:
Not specified
Region / city:
Not specified
Subject:
Serum protein binding, small proteins
Document type:
Protocol
Organization / institution:
Not specified
Author:
Not specified
Target audience:
Researchers, scientists
Effective period:
Not specified
Approval date:
Not specified
Modification date:
Not specified
Year:
2021
Region / city:
Queensland, Australia
Topic:
miRNA expression analysis, pleural effusions, extracellular vesicles
Document type:
Research article
Institution:
The University of Queensland Thoracic Research Centre, The Prince Charles Hospital
Authors:
Tian Mun Chee, Caeli J. Zahra, Kwun M. Fong, Ian A. Yang, Rayleen V. Bowman
Target audience:
Researchers in molecular biology, oncology
Period of validity:
N/A
Approval date:
N/A
Date of changes:
N/A
Knockeen Trenching and New Assay Sampling Programme to Commence at Leinster Lithium Project, Ireland
Year:
2023
Region / City:
Leinster, Ireland
Theme:
Lithium exploration, Mining
Document Type:
Press Release
Organization:
Technology Minerals Plc, Global Battery Metals Ltd
Author:
Alex Stanbury, Michael Murphy
Target Audience:
Investors, Industry professionals
Period of validity:
Up to two years
Approval Date:
23 November 2023
Amendment Date:
-
Year:
2021
Region / city:
Towers Lab, International
Topic:
HIV Research, DNA-Sensing Pathways, Viral Proteins
Document Type:
Scientific Research Paper
Organization / Institution:
Towers Lab
Author:
James Cai
Target Audience:
Researchers, Scientists, Molecular Biologists
Period of Action:
Not specified
Date of Approval:
Not specified
Date of Modifications:
Not specified
Product Name:
6-Phosphogluconate Dehydrogenase Assay Kit (ab241016)
Version:
2a
Last Updated:
24 August 2023
Manufacturer:
Abcam
Former Brand:
BioVision
Intended Use:
Research use only, not for diagnostic use
Analyte:
6-Phosphogluconate Dehydrogenase (6-PGD)
Detection Method:
Colorimetric, absorbance at 460 nm
Sample Types:
Tissue homogenates; adherent and suspension cells
Storage Conditions:
-20°C, protected from light
Kit Stability:
1 year from receipt if components are not reconstituted
Assay Temperature:
37°C
Measurement Mode:
Kinetic and endpoint spectrophotometric analysis
Unit Definition:
One unit generates 1.0 µmole of NADPH per min at pH 8.0 at 37°C
Document Type:
Laboratory assay kit instruction manual
Copyright:
© 2023 Abcam. All Rights Reserved.