№ lp_1_2_45961
File format: docx
Character count: 8095
File size: 28 KB
Year:
2023
Region / city:
Global
Topic:
Cell proliferation assay
Document type:
Kit instruction manual
Organization:
Abcam
Author:
Abcam Technical Team
Target audience:
Researchers, lab technicians
Period of validity:
1 year from receipt
Approval date:
2023
Date of modifications:
None
Storage conditions:
Store at -20°C in the dark
Components:
MTT Reagent, MTT Solvent
Required materials (not supplied):
Microplate reader, pipettes, 96 well plate
Safety notes:
Wear gloves, lab coat, and protective eyewear; handle reagents with care
Assay procedure:
Detailed protocol for measuring cell proliferation using MTT reagent
Interferences:
Serum or phenol red in culture medium may cause background interference
FAQ:
Instructions for background control, handling of MTT reagent, and troubleshooting
Contextual description:
Instruction manual for a research kit used to measure cell proliferation in cultured cells.
Price: 8 / 10 USD
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Event Name:
MTT August 16 and 17 2025 – ALL
Dates:
August 16–17, 2025
Type of Document:
Turn-by-turn route directions
Activity:
Running
Total Distance:
10.16 mi
Distance Unit:
Miles
Location:
Richmond, Virginia
Start Point:
Head through green lot to Robin Hood Rd
End Point:
Squirrels Way
SAG Locations:
Grace and Ryland; 10th and Byrd; Colorado and Greenville; Robinson and Paddle Boat Lane
Source Platforms:
MapMyRun; RunGo; GPX file (Google Drive)
Format:
Sequential street navigation with cumulative mileage markers
Date:
November 5-6
Event:
MTT
Teams:
All
Route:
Avenue of Champions to Hollywood Cemetery
Platform:
MapMyRun
Link:
MapMyRun Route
Note:
Link
RunGo Route
File:
Google Drive File
Year:
2019
Region / City:
Saint Lucia
Topic:
Anti-money laundering, Counter financing of terrorism, Remittance services
Document Type:
Report
Organization / Institution:
Attorney General’s Chambers, National Anti-Money Laundering Oversight Committee (NAMLOC), Financial Intelligence Authority, Financial Services Regulatory Authority
Author:
Attorney General’s Chambers and the National Anti-Money Laundering Oversight Committee (NAMLOC)
Target Audience:
Policymakers, Financial Institutions, Compliance Officers
Period of Validity:
Ongoing
Approval Date:
N/A
Date of Changes:
N/A
Note:
Description
Year:
2022
Region / City:
Los Angeles, California
Topic:
Nuclear Proliferation
Document Type:
Course Syllabus
Institution:
California State University, Los Angeles
Author:
Adal A Isaw
Target Audience:
Students of Political Science and International Relations
Period of Validity:
Fall 2022
Approval Date:
Not specified
Modification Date:
Not specified
Year:
2026
Region / City:
Hong Kong SAR
Subject:
Neuroscience, Biomedical Sciences, Drug Delivery
Document Type:
Research Article
Organization / Institution:
City University of Hong Kong
Author:
Abhimanyu Thakur, Rakesh Kumar Sidu, Heng Zou, Md Kowsar Alam, Mengsu Yang, Youngjin Lee
Target Audience:
Researchers, Scientists, Medical Professionals
Period of Validity:
N/A
Approval Date:
N/A
Modification Date:
N/A
Year:
2023
Region / City:
Indianapolis, IN
Topic:
Periodontal Regeneration
Document Type:
Research Article
Institution:
Indiana University School of Dentistry
Authors:
Raziel Velgis, L. Jack Windsor, Vanchit John, Chandni Batra
Target Audience:
Researchers, Dental Professionals
Period of Validity:
N/A
Approval Date:
N/A
Modification Date:
N/A
Keywords:
rhPDGF-BB, VEGF, Periodontal Ligament Cells, Proliferation, Regeneration
Context:
A research article investigating the effects of recombinant human platelet-derived growth factor-BB (rhPDGF-BB) on the proliferation of periodontal ligament cells and VEGF expression.
Document type:
Supplementary scientific figures
Field:
Immunology
Research topic:
Dendritic cells, antigen uptake, T cell proliferation, and sarcosine effects in tumor models
Experimental model:
Murine bone marrow–derived dendritic cells (BM-DC) and B16F10-OVA tumor-bearing mice
Biological materials:
FITC-OVA antigen, OVA-mRNA electroporated dendritic cells, sarcosine treated dendritic cells
Methods:
Antigen uptake measurement by mean fluorescence intensity (MFI), in vitro T cell proliferation assay, co-culture experiments, tumor implantation and monitoring
Statistical analysis:
Unpaired t test; two-way ANOVA
Sample size:
n=3; n=5; n=10
Measured indicators:
Antigen uptake (MFI), T cell proliferation percentage, body weight, tumor volume
Experimental comparison groups:
Control BM-DC, untreated BM-DC with OVA-mRNA electroporation, sarcosine treated BM-DC with OVA-mRNA electroporation, sarcosine loaded tumor conditions
Organism:
Mouse
Year:
2023
Author:
Massimiliano Simons
Institution:
KU Leuven
Field:
Philosophy of Science / Science and Technology Studies
Document Type:
Academic Article
Keywords:
Bachelard, phénoménotechnique, epistemological rupture, purification, proliferation, science studies, Latour, Althusser
Audience:
Scholars and students in philosophy of science and STS
Abstract:
Analyzes the relationship between Bachelard’s concepts of epistemological break and phénoménotechnique, exploring historical interpretations and contemporary relevance
Period Covered:
1960s–1980s (historical uses), theoretical discussion up to 2023
Year:
2023
Region / City:
-
Subject:
Immunology, Diagnostic Assay
Document Type:
Technical Manual
Organization:
-
Author:
-
Target Audience:
Laboratory Professionals
Period of Validity:
-
Approval Date:
-
Modification Date:
-
Year:
2025
Note:
Region / City
Theme:
Seed amplification assay innovation
Document Type:
Application Instructions
Target Audience:
Researchers in scientific and clinical fields
Effective Period:
Spring 2025
Document type:
Materials and Methods section
Species:
C57Bl6/J mice; Sprague Dawley rats
Animal source:
The Jackson Laboratories (Bar Harbor, Maine); Zhejiang Vital River Laboratory Animal Technology Co., Ltd.
Experimental facilities:
BioDuro-Sundia (Shanghai, China)
Ethics approval:
IACUC approved protocols
Regulatory compliance:
Animal Welfare Act (9 CFR Parts 1–3); Guide for the Care and Use of Laboratory Animals (8th Edition); AAALAC accreditation (001516)
Cell lines:
YPMK; YPMK PINK1 KO; EPF1; HEK293 PINK1 KO; SK-OV-3; ΔOTC
Primary cultures:
Mouse hippocampal and cortical neurons (E16, A53T α-synuclein M83 mice)
Key assay:
SMCxPRO pUb assay
Key reagents:
Rabbit anti-pS65-Ub antibody (CST E2J6T); Mouse anti-Ub antibody (CST P4D1); K48 pUb tetramers (RnD Systems UC-250)
Instrumentation:
SMCxPRO system; Agilent Seahorse XFe96 analyzer; ImageXpress Micro Confocal High-Content Imaging System
Experimental focus:
α-synuclein preformed fibrils (PFFs); mitochondrial respiration; mitophagy measurement (MT-mKeima)
Data availability:
Available from corresponding author upon reasonable request under Material Transfer Agreement
Code availability:
No original code reported
Note:
Year
Subject:
Pharmacology
Document type:
Research methodology
Target audience:
Researchers, Pharmacologists
Description:
This document provides the experimental setup, assay parameters, and performance data for the analysis of venetoclax and voriconazole in a pharmacokinetic study.
Year:
Not specified
Region / city:
Not specified
Subject:
Serum protein binding, small proteins
Document type:
Protocol
Organization / institution:
Not specified
Author:
Not specified
Target audience:
Researchers, scientists
Effective period:
Not specified
Approval date:
Not specified
Modification date:
Not specified
Year:
2021
Region / city:
Queensland, Australia
Topic:
miRNA expression analysis, pleural effusions, extracellular vesicles
Document type:
Research article
Institution:
The University of Queensland Thoracic Research Centre, The Prince Charles Hospital
Authors:
Tian Mun Chee, Caeli J. Zahra, Kwun M. Fong, Ian A. Yang, Rayleen V. Bowman
Target audience:
Researchers in molecular biology, oncology
Period of validity:
N/A
Approval date:
N/A
Date of changes:
N/A
Knockeen Trenching and New Assay Sampling Programme to Commence at Leinster Lithium Project, Ireland
Year:
2023
Region / City:
Leinster, Ireland
Theme:
Lithium exploration, Mining
Document Type:
Press Release
Organization:
Technology Minerals Plc, Global Battery Metals Ltd
Author:
Alex Stanbury, Michael Murphy
Target Audience:
Investors, Industry professionals
Period of validity:
Up to two years
Approval Date:
23 November 2023
Amendment Date:
-
Year:
2021
Region / city:
Towers Lab, International
Topic:
HIV Research, DNA-Sensing Pathways, Viral Proteins
Document Type:
Scientific Research Paper
Organization / Institution:
Towers Lab
Author:
James Cai
Target Audience:
Researchers, Scientists, Molecular Biologists
Period of Action:
Not specified
Date of Approval:
Not specified
Date of Modifications:
Not specified
Product Name:
6-Phosphogluconate Dehydrogenase Assay Kit (ab241016)
Version:
2a
Last Updated:
24 August 2023
Manufacturer:
Abcam
Former Brand:
BioVision
Intended Use:
Research use only, not for diagnostic use
Analyte:
6-Phosphogluconate Dehydrogenase (6-PGD)
Detection Method:
Colorimetric, absorbance at 460 nm
Sample Types:
Tissue homogenates; adherent and suspension cells
Storage Conditions:
-20°C, protected from light
Kit Stability:
1 year from receipt if components are not reconstituted
Assay Temperature:
37°C
Measurement Mode:
Kinetic and endpoint spectrophotometric analysis
Unit Definition:
One unit generates 1.0 µmole of NADPH per min at pH 8.0 at 37°C
Document Type:
Laboratory assay kit instruction manual
Copyright:
© 2023 Abcam. All Rights Reserved.
Year:
2020
Region / City:
United Kingdom
Topic:
Biochemical Assay
Document Type:
Datasheet
Organization:
Abcam
Author:
Abcam
Target Audience:
Researchers
Period of Validity:
N/A
Approval Date:
N/A
Date of Changes:
N/A