№ lp_1_2_44278
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This document outlines the validation procedure for the method xxx, detailing the necessary parameters and criteria for assessing accuracy, precision, specificity, and other relevant aspects of the method.
Note:
Year
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The product description is provided for reference. Actual content and formatting may differ slightly.
Year:
2023
Region / City:
-
Subject:
Immunology, Diagnostic Assay
Document Type:
Technical Manual
Organization:
-
Author:
-
Target Audience:
Laboratory Professionals
Period of Validity:
-
Approval Date:
-
Modification Date:
-
Year:
2025
Note:
Region / City
Theme:
Seed amplification assay innovation
Document Type:
Application Instructions
Target Audience:
Researchers in scientific and clinical fields
Effective Period:
Spring 2025
Year:
2023
Region / city:
Global
Topic:
Cell proliferation assay
Document type:
Kit instruction manual
Organization:
Abcam
Author:
Abcam Technical Team
Target audience:
Researchers, lab technicians
Period of validity:
1 year from receipt
Approval date:
2023
Date of modifications:
None
Storage conditions:
Store at -20°C in the dark
Components:
MTT Reagent, MTT Solvent
Required materials (not supplied):
Microplate reader, pipettes, 96 well plate
Safety notes:
Wear gloves, lab coat, and protective eyewear; handle reagents with care
Assay procedure:
Detailed protocol for measuring cell proliferation using MTT reagent
Interferences:
Serum or phenol red in culture medium may cause background interference
FAQ:
Instructions for background control, handling of MTT reagent, and troubleshooting
Contextual description:
Instruction manual for a research kit used to measure cell proliferation in cultured cells.
Document type:
Materials and Methods section
Species:
C57Bl6/J mice; Sprague Dawley rats
Animal source:
The Jackson Laboratories (Bar Harbor, Maine); Zhejiang Vital River Laboratory Animal Technology Co., Ltd.
Experimental facilities:
BioDuro-Sundia (Shanghai, China)
Ethics approval:
IACUC approved protocols
Regulatory compliance:
Animal Welfare Act (9 CFR Parts 1–3); Guide for the Care and Use of Laboratory Animals (8th Edition); AAALAC accreditation (001516)
Cell lines:
YPMK; YPMK PINK1 KO; EPF1; HEK293 PINK1 KO; SK-OV-3; ΔOTC
Primary cultures:
Mouse hippocampal and cortical neurons (E16, A53T α-synuclein M83 mice)
Key assay:
SMCxPRO pUb assay
Key reagents:
Rabbit anti-pS65-Ub antibody (CST E2J6T); Mouse anti-Ub antibody (CST P4D1); K48 pUb tetramers (RnD Systems UC-250)
Instrumentation:
SMCxPRO system; Agilent Seahorse XFe96 analyzer; ImageXpress Micro Confocal High-Content Imaging System
Experimental focus:
α-synuclein preformed fibrils (PFFs); mitochondrial respiration; mitophagy measurement (MT-mKeima)
Data availability:
Available from corresponding author upon reasonable request under Material Transfer Agreement
Code availability:
No original code reported
Note:
Year
Subject:
Pharmacology
Document type:
Research methodology
Target audience:
Researchers, Pharmacologists
Description:
This document provides the experimental setup, assay parameters, and performance data for the analysis of venetoclax and voriconazole in a pharmacokinetic study.
Year:
Not specified
Region / city:
Not specified
Subject:
Serum protein binding, small proteins
Document type:
Protocol
Organization / institution:
Not specified
Author:
Not specified
Target audience:
Researchers, scientists
Effective period:
Not specified
Approval date:
Not specified
Modification date:
Not specified
Year:
2021
Region / city:
Queensland, Australia
Topic:
miRNA expression analysis, pleural effusions, extracellular vesicles
Document type:
Research article
Institution:
The University of Queensland Thoracic Research Centre, The Prince Charles Hospital
Authors:
Tian Mun Chee, Caeli J. Zahra, Kwun M. Fong, Ian A. Yang, Rayleen V. Bowman
Target audience:
Researchers in molecular biology, oncology
Period of validity:
N/A
Approval date:
N/A
Date of changes:
N/A
Knockeen Trenching and New Assay Sampling Programme to Commence at Leinster Lithium Project, Ireland
Year:
2023
Region / City:
Leinster, Ireland
Theme:
Lithium exploration, Mining
Document Type:
Press Release
Organization:
Technology Minerals Plc, Global Battery Metals Ltd
Author:
Alex Stanbury, Michael Murphy
Target Audience:
Investors, Industry professionals
Period of validity:
Up to two years
Approval Date:
23 November 2023
Amendment Date:
-
Year:
2021
Region / city:
Towers Lab, International
Topic:
HIV Research, DNA-Sensing Pathways, Viral Proteins
Document Type:
Scientific Research Paper
Organization / Institution:
Towers Lab
Author:
James Cai
Target Audience:
Researchers, Scientists, Molecular Biologists
Period of Action:
Not specified
Date of Approval:
Not specified
Date of Modifications:
Not specified
Product Name:
6-Phosphogluconate Dehydrogenase Assay Kit (ab241016)
Version:
2a
Last Updated:
24 August 2023
Manufacturer:
Abcam
Former Brand:
BioVision
Intended Use:
Research use only, not for diagnostic use
Analyte:
6-Phosphogluconate Dehydrogenase (6-PGD)
Detection Method:
Colorimetric, absorbance at 460 nm
Sample Types:
Tissue homogenates; adherent and suspension cells
Storage Conditions:
-20°C, protected from light
Kit Stability:
1 year from receipt if components are not reconstituted
Assay Temperature:
37°C
Measurement Mode:
Kinetic and endpoint spectrophotometric analysis
Unit Definition:
One unit generates 1.0 µmole of NADPH per min at pH 8.0 at 37°C
Document Type:
Laboratory assay kit instruction manual
Copyright:
© 2023 Abcam. All Rights Reserved.
Year:
2020
Region / City:
United Kingdom
Topic:
Biochemical Assay
Document Type:
Datasheet
Organization:
Abcam
Author:
Abcam
Target Audience:
Researchers
Period of Validity:
N/A
Approval Date:
N/A
Date of Changes:
N/A
Year:
2023
Region / city:
Parkville, Victoria, Australia
Topic:
Immunofluorescence, laboratory techniques
Document type:
Laboratory protocol
Organization:
Walter and Eliza Hall Institute of Medical Research
Author:
Thanh Ngoc Nguyen
Target audience:
Laboratory researchers, biotechnologists
Date of approval:
Not specified
Date of changes:
Not specified
Year:
N/A
Region / City:
N/A
Subject:
Biochemical Analysis
Document Type:
Methodology
Institution:
N/A
Author:
N/A
Target Audience:
Laboratory technicians, researchers
Period of Use:
N/A
Approval Date:
N/A
Date of Revision:
N/A
This document describes a biochemical method for measuring ALT (SGPT) activity in serum samples using two different assay techniques:
discontinuous (colorimetric) and continuous (UV/kinetic).
Year:
2019
Region / City:
United States
Subject:
Toxicology Testing
Document Type:
Data Release
Organization / Institution:
United States Environmental Protection Agency (EPA)
Author:
Monica Linnenbrink
Target Audience:
Researchers, Toxicologists, Environmental Scientists
Period of Action:
March 2019
Approval Date:
March 2019
Revision Date:
Not specified
Institution:
University of Manitoba Max Rady College of Medicine
Program:
XXX Residency Program
Document type:
Policy
Topic:
Fatigue risk management in residency training
Applies to:
Postgraduate medical trainees
Scope:
Clinical and academic activities during residency
Responsible roles:
Program Director, residents, Fatigue Risk Management Local Working Group
Approval status:
Approved
Review cycle:
Periodic review indicated
Geographic context:
Manitoba, Canada
Policy focus:
Health, safety, and patient care quality
Year:
2026
Region / city:
[University Name]
Topic:
SHPE Chapter Operations and Membership Guidelines
Document Type:
Template
Organization:
SHPE
Author:
SHPE National
Target audience:
Student members of SHPE
Effective period:
From chapter formation to approval by SHPE National
Approval date:
Not specified
Date of amendments:
Not specified
Note:
Year
Year:
2013
Note:
Region / City
Theme:
Matrimony
Document Type:
Template
Organization / Institution:
Liturgy Office
Target Audience:
Couples preparing for marriage
Context:
Template for a Wedding Order of Service used to guide the celebration of a wedding Mass with responses for those unfamiliar with the Mass.
Year:
XXXX
Region / City:
XXX
Topic:
Adjuvant Therapy for XXX
Document Type:
Executive Summary
Organization / Institution:
American Radium Society
Author:
XXX, MD1; YYY, MD2; ZZZ, MD3
Target Audience:
Healthcare professionals in oncology
Period of validity:
XXXX–XXXX
Approval Date:
XXXX
Date of Changes:
N/A