№ files_lp_3_process_9_28034
Laboratory protocol detailing the composition, preparation procedure, safety measures, quality controls and formulation comparison for Artificial Cerebrospinal Fluid V (ACSF.V) used in surgical and experimental settings.
Reagent Name: Artificial Cerebrospinal Fluid V (ACSF.V)
Document Type: Laboratory preparation protocol
Application: Surgical procedures to maintain tissue hydration and minimize tissue damage, adhesion formation and infection
Final Volume: 1000 ml
Final pH: 7.4
Storage Conditions: 4°C
Shelf Life: One month at 4°C
Output Composition: 135.0 mM NaCl; 5.4 mM KCl; 1.0 mM MgCl2; 1.8 mM CaCl2; 5.0 mM HEPES
Required Equipment: pH meter; calibrated balance; stir plate; Gast vacuum pump (Model DOA-P104-AA or equivalent); fume hood
Filtration Specification: 0.22 µm filter system (Corning 431206 or equivalent)
Safety Requirements: Gloves; eye protection; lab coat; fume hood when handling KOH
Hazard Information: Potassium Hydroxide (KOH) can cause burns
Reference Documents: EQ0006 pH Meter Calibration and Usage; EQ0020 Balance Calibration Validation
Disposal Method: Excess reagent disposed into municipal sewer system
Osmolarity Control: Measured upon request using osmometer; adjusted with MilliQ water or 1 mg/ml NaCl solution
Price: 8 / 10 USD
The file will be delivered to the email address provided at checkout within 12 hours.

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