№ lp_2_3_27366
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File size: 187 KB
Step-by-step laboratory protocol detailing the collection, transport, and microscopic and biochemical examination of cerebrospinal fluid to detect bacterial and other infectious causes of meningitis.
Year:
Not specified
Region / City:
Not specified
Subject:
Bacterial meningitis diagnosis
Document type:
Laboratory protocol / Standard operating procedure
Institution:
Not specified
Target audience:
Laboratory personnel / medical staff
Specimen type:
Cerebrospinal fluid
Methods included:
Cell counts, macroscopic examination, Gram staining, Giemsa staining, India ink staining
Applicable age groups:
Neonates, infants, children, adults, immunocompromised
Pathogens covered:
Group B Streptococci, E. coli, Listeria monocytogenes, Haemophilus influenzae type B, Neisseria meningitidis, Streptococcus pneumoniae, Cryptococcus neoformans, Mycobacterium tuberculosis, Streptococcus suis
Safety precautions:
Class II biosafety cabinet use, aseptic handling
Reference tables/appendices:
Appendix 1 (normal CSF cell counts), Table 1 (organism identification)
Laboratory equipment:
Fuchs-Rosenthal counting chamber, centrifuge, microscope
Price: 8 / 10 USD
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The product description is provided for reference. Actual content and formatting may differ slightly.
Year:
2026
Journal:
Journal of Medicine and Health Research
Manuscript Number:
Ms_JOMAHR_14356
Type of Article:
Review Article
Author:
Not specified
Reviewer:
Yerko Petar Ivanovic-Barbeito
Affiliation:
Tallaght University Hospital, Ireland
Target Audience:
Pediatricians, infectious disease physicians, neurologists, global health practitioners
Timeframe Covered:
2000–2025
Language:
English
Ethical Considerations:
No human subjects involved
Note:
Year
Reagent Name:
Artificial Cerebrospinal Fluid V (ACSF.V)
Document Type:
Laboratory preparation protocol
Application:
Surgical procedures to maintain tissue hydration and minimize tissue damage, adhesion formation and infection
Final Volume:
1000 ml
Final pH:
7.4
Storage Conditions:
4°C
Shelf Life:
One month at 4°C
Output Composition:
135.0 mM NaCl; 5.4 mM KCl; 1.0 mM MgCl2; 1.8 mM CaCl2; 5.0 mM HEPES
Required Equipment:
pH meter; calibrated balance; stir plate; Gast vacuum pump (Model DOA-P104-AA or equivalent); fume hood
Filtration Specification:
0.22 µm filter system (Corning 431206 or equivalent)
Safety Requirements:
Gloves; eye protection; lab coat; fume hood when handling KOH
Hazard Information:
Potassium Hydroxide (KOH) can cause burns
Reference Documents:
EQ0006 pH Meter Calibration and Usage; EQ0020 Balance Calibration Validation
Disposal Method:
Excess reagent disposed into municipal sewer system
Osmolarity Control:
Measured upon request using osmometer; adjusted with MilliQ water or 1 mg/ml NaCl solution
Year:
2021
Region / City:
United Kingdom
Subject:
Health economics, antimicrobial evaluation
Document type:
Final report
Organization / Institution:
Centre for Health Economics, University of York; Health Economics and Decision Science, University of Sheffield
Author:
Sue Harnan, Ben Kearns, Alison Scope, Laetitia Schmitt, Dina Jankovic, Jean Hamilton, Ruth Wong, Tushar Srivastava, Harry Hill, Chu Chang Ku, Kate Ren, Claire Rothery, Laura Bojke, Mark Sculpher, Beth Woods
Target audience:
Healthcare professionals, policymakers, researchers
Period of validity:
2016 - ongoing
Approval date:
October 2021
Date of last modification:
October 2021
Document type:
Supplementary material
Scientific field:
Molecular biology
Research focus:
Anticalin selection and affinity maturation
Target protein:
Membrane-bound Hsp70
Methodology:
Bacterial surface display
Techniques:
FACS screening, affinity maturation, directed evolution, SPR, CD spectroscopy, flow cytometry
Biological models:
E. coli, FaDu cells, OPM-2 cells
Authors:
Lars Friedrich; Petra Kornberger; Claudia T. Mendler; Gabriele Multhoff; Markus Schwaiger; Arne Skerra
Figures included:
Figures S1–S7
Related publication:
Main research article on Anticalin selection against Hsp70
Experimental scope:
In vitro binding analysis and cellular characterization
Document type:
Laboratory exercise instructions
Subject area:
Microbiology
Educational level:
Undergraduate laboratory
Focus:
Bacterial growth media and culturing techniques
Topics covered:
Normal flora, aseptic technique, selective and differential media, colony morphology, hemolysis
Media discussed:
TSY agar, MacConkey’s agar, Mannitol Salt agar, Blood agar
Biological concepts:
Normal flora, bacterial colonies, hemolysis, Gram-positive and Gram-negative bacteria
Laboratory context:
Teaching laboratory
Associated organisms:
Staphylococcus, Streptococcus, Enterobacteriaceae
Intended audience:
Students enrolled in a microbiology laboratory course
Temporal context:
Multi-week laboratory sequence
Instructional purpose:
Observation and analysis of bacterial growth on culture media
Year:
2023
Region / City:
Not specified
Topic:
Microbiology, Genetic Engineering
Document Type:
Supplementary Information
Organization / Institution:
Not specified
Author:
Wolf M, Geczi A, Simon O, Borriss R
Target Audience:
Researchers in microbiology and genetic engineering
Effective Period:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Year:
2026
Institution:
University of Leicester
College:
College of Life Sciences
School/Department:
LeMID
Funding:
CLS / HPRU Grant studentship
First Supervisor:
Prof Julie Morrissey
Second Supervisor:
Dr Emma Marczylo, UK Health Security Agency (UKHSA)
Additional Supervisors:
Kate Jones, Health and Safety Executive; Emma-Jane Goode
Project Type:
PhD research project
Field:
Microbiology / Public Health
Focus:
Metal exposure, bacterial persistence, antimicrobial resistance
Target Organisms:
Staphylococcus aureus (CA-MRSA and HA-MRSA)
Training Provided:
Molecular microbiology, tissue culture, omics, microscopy
Collaborating Organizations:
Health Protection Research Unit, National Institute of Health Research (NIHR), Health and Safety Executive, UKHSA
Note:
Year
Topic:
Blood culture, bacterial infection, microbiology
Document type:
Procedure
Target audience:
Medical professionals, laboratory technicians
Year:
2023
Region / City:
Salt Lake City, Utah, United States
Subject:
Bacterial diversity, chemical ecology, natural products
Document Type:
Scientific Research Paper
Organization / Institution:
University of Utah, Scripps Institution of Oceanography, University of California at San Diego
Author:
Elijah R. Bring Horvath, William J. Brazelton, Min Cheol Kim, Reiko Cullum, Matthew A. Mulvey, William Fenical, Jaclyn M. Winter
Target Audience:
Researchers in microbiology, marine biology, and chemical ecology
Period of Validity:
N/A
Approval Date:
N/A
Date of Changes:
N/A
Year:
2018
Region / City:
Not specified
Topic:
Gene expression in biofilm and planktonic bacteria
Document type:
Scientific article
Organization / Institution:
Not specified
Author:
Not specified
Target audience:
Researchers in microbiology and molecular biology
Effective period:
Not specified
Approval date:
Not specified
Modification date:
Not specified
Document type:
Supporting information
Related article title:
Organic fertilizers shape the bacterial communities harboring pqqC and phoD genes by altering organic acids, leading to improved phosphorus utilization
Authors:
Liying Zhi; Bangxiao Zheng; Yunjie Xu; Jiayang Xu; Josep Peñuelas; Jordi Sardans; Yixiao Chang; Shuquan Jin; Hong Ying; Kai Ding
Affiliations:
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China; College of JunCao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, China; School of Environmental Science and Engineering, Xiamen University of Technology, Xiamen, China; Bureau of Agriculture and Rural Affairs, Wencheng, China; University of Chinese Academy of Sciences, Beijing, China; CSIC, Global Ecology Unit, CREAF-CSIC-UAB, Bellaterra, Spain; CREAF, Cerdanyola del Vallès, Spain; Institute of Ecological Environment, Ningbo Academy of Agricultural Sciences, Ningbo, China; Yangtze Delta Region Healthy Agricultural Institute (Zhejiang) Co., Ltd, Tongxiang, China
Number of pages:
12
Number of figures:
9
Number of tables:
5
Subject area:
Soil microbiology
Keywords:
organic fertilizer; inorganic fertilizer; pqqC gene; phoD gene; bacterial community; soil physicochemical properties; organic acids; phosphorus activation rate
Experimental design:
Fertilizer treatment groups including chemical and organic amendments
Analytical methods:
PCOA based on Bray-Curtis dissimilarity; Spearman correlation analysis; quantitative PCR; soil physicochemical and enzyme assays
Gene targets:
16S rRNA; pqqC; phoD
Measured parameters:
Soil pH; SOC; TN; TP; TK; AN; AP; AK; organic acid content; enzyme activities (ACP, ALP)
Year:
2006
Region / city:
Los Angeles, California
Subject:
Water quality, bacteria standards
Document type:
Amendment
Authority / institution:
Regional Water Quality Control Board, State Water Resources Control Board, U.S. Environmental Protection Agency
Author:
Not specified
Target audience:
Environmental professionals, policymakers, water quality regulators
Effective period:
June 8, 2006, and subsequent revisions
Approval date:
June 8, 2006
Revision date:
June 7, 2012, and later revisions
Context:
This document presents the revision of the Total Maximum Daily Load (TMDL) for bacterial indicator densities in water bodies in the Los Angeles region, with specific provisions for different water types and recreation uses.
Year:
2023
Region / city:
Seattle, WA, USA
Topic:
Microbiology, Epidemiology, Infectious Diseases
Document type:
Research Article
Organization / institution:
University of Washington, Fred Hutchinson Cancer Research Center
Author:
Kayla A. Carter, Molly D. Fischer, Mariya I. Petrova, Jennifer E. Balkus
Target audience:
Researchers, Public Health Professionals
Period of validity:
Not specified
Date of approval:
Not specified
Date of changes:
Not specified
Year:
2023
Region / City:
UK
Theme:
Medical Treatment, Patient Care
Document Type:
Patient Assessment Form, Medical Protocol
Organization:
NHS Pharmacy First Scotland
Author:
NHS Pharmacy
Target Audience:
Healthcare Professionals, Pharmacists
Effective Period:
Indefinite
Approval Date:
N/A
Date of Changes:
N/A
Treatment Method:
Flucloxacillin administration
Exclusion Criteria:
Hypersensitivity to beta-lactam antibiotics, recent antibiotic use for the same condition, pregnancy, and several other medical conditions
Medicines Supplied:
Flucloxacillin capsules, oral solution
Instructions for Use:
Dosage guidance, patient advice checklist
Patient Information:
Consent required for treatment
Confidentiality Notice:
Yes
Contact:
GP practice and pharmacy details
Year:
2021
Region / City:
Ann Arbor, MI, USA
Subject:
Structural Biology, RNA Biomedicine
Document Type:
Research Paper
Organization:
University of Michigan, Life Sciences Institute, Single Molecule Analysis Group, Biophysics Program
Authors:
Adrien Chauvier, Jason C. Porta, Indrajit Deb, Emily Ellinger, Aaron T. Frank, Melanie D. Ohi, Nils G. Walter
Target Audience:
Researchers in the field of biochemistry and molecular biology
Period of Validity:
N/A
Approval Date:
N/A
Modification Date:
N/A
Year:
1999
Note:
Region / city
Topic:
Bacterial genetics, plasmids
Document type:
Research paper
Author:
Weiss et al., 1999; Beaupré et al., 1997; Datsenko and Wanner, 2000; Kang et al., 2005; Roggiani and Goulian, 2015
Target audience:
Researchers in molecular biology and genetics