№ lp_2_1_01209
Technical veterinary laboratory protocol outlining procedures, conditions, biosafety measures and transport requirements for collection and preservation of oro-pharyngeal fluid samples from ruminants for detection and characterization of foot-and-mouth disease virus.
Subject: Probang sampling and oro-pharyngeal fluid (OP Fluid) collection and preservation
Sample type: Oro-pharyngeal fluid containing cellular material
Target disease: Foot-and-mouth disease (FMD)
Target pathogen: FMD virus (serotype/genotype)
Target animal group: Susceptible ruminants (primarily cattle and buffalo; sheep and goats where appropriate equipment is available)
Study objectives: Post-outbreak investigations; detection of subclinical and carrier infections; historical infection demonstration; virus characterization
Diagnostic methods: RT-PCR; real-time RT-PCR; virus isolation
Time frame for sampling: Incubation period; 0–10 days; 11–28 days; >28 days after onset of clinical signs
Required equipment: Probang cups; sterile screw-cap tubes; transport medium (pH 7.2–7.6); disinfectants; ice boxes; liquid nitrogen
Sample storage conditions: 4°C during transport; < -20°C (preferably -80°C) for storage; dry ice or liquid nitrogen vapour
Biosafety requirements: Disinfection of equipment and containers; compliance with regulations for transportation of infectious substances
Geographical references: China; Thailand; Republic of Korea
Intended institutions: Field units; national laboratory
Price: 8 / 10 USD
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