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This document contains supplementary figures supporting research on macrophage viability and cytotoxicity after exposure to crystalline silica particles, including gating strategies and immune cell population analysis.
Year:
2023
Region / City:
Not specified
Topic:
Immunology, Cytotoxicity, Flow Cytometry
Document Type:
Research Supplementary Figures
Author:
Not specified
Target Audience:
Researchers, Immunologists
Period of Validity:
Not specified
Approval Date:
Not specified
Modification Date:
Not specified
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Authors:
Jianhua Rao; Jiannan Qiu; Ming Ni; Hao Wang; Peng Wang; Lei Zhang; Zeng Wang; Mu Liu; Feng Cheng; Xuehao Wang; Ling Lu
Affiliations:
Hepatobiliary Center of The Affiliated Cancer Hospital (Jiangsu Cancer Hospital) and The First Affiliated Hospital, Nanjing Medical University; Research Unit of Liver Transplantation and Transplant Immunology, Chinese Academy of Medical Sciences; Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment; Collaborative Innovation Center for Personalized Cancer Medicine; State Key Laboratory of Reproductive Medicine
Institution:
Nanjing Medical University
Country:
China
Type of Document:
Supplementary Material
Discipline:
Immunology; Hepatology; Molecular Biology
Subject:
Role of macrophage Nrf2 deficiency and Timp3-mediated RhoA/ROCK pathway in ischemic liver injury
Experimental Models:
Mouse models; Bone marrow–derived macrophages (BMMs); Hepatic macrophages
Methods:
Cell isolation and culture; Biochemical measurements; Histopathology; Immunohistochemistry; Immunofluorescence; TUNEL staining; MDA, GSH and MPO assays; Western blotting; qRT-PCR; ELISA; RNA sequencing; Chromatin immunoprecipitation
Correspondence:
Ling Lu; Xuehao Wang; Feng Cheng
Equal Contribution:
Jianhua Rao; Jiannan Qiu; Ming Ni
Year:
2026
Species:
Mus musculus
Sex:
Male and Female
Strains:
LLC, NOD-SCID
Experimental procedure:
Transverse aortic constriction (TAC) with and without tumor implantation
Measured parameters:
Fractional shortening, ventricles/body weight ratio, mRNA levels of hypertrophic and fibrosis markers, macrophage polarization markers
Techniques:
Echocardiography, qRT-PCR, flow cytometry, immunohistochemistry
Statistical analysis:
Student t-test, results expressed as mean ± SEM
Markers analyzed:
ANP, BNP, ACTA1, Collagen, TGFβ3, CTGF, SMA, iNOS, CD206, ARG1, F4/80, CD163, INFγ, IL-13, CCL2, TNFα
Supplemental tables:
Oligonucleotide sequences, echocardiography parameters
Supplemental figures:
6 figures detailing cardiac function, macrophage polarization, and tumor growth effects
Year:
2026
Region / City:
Not specified
Topic:
Bone Regeneration, Macrophage Phenotype Switching
Document Type:
Research Article
Organization / Institution:
Not specified
Author:
Not specified
Target Audience:
Researchers, Medical Professionals
Duration:
Not specified
Approval Date:
Not specified
Modification Date:
Not specified
Research Field:
Immunology
Subfield:
Host Defense and Sepsis
Experimental Model:
C57BL/6 and C57BL/6J mice
Biological Focus:
Macrophage function and bacterial clearance
Bacterial Species:
Pseudomonas aeruginosa
Laboratory Techniques:
CFU determination, ELISA, flow cytometry, Western blot, siRNA interference, fluorescence microscopy
Cell Type:
Mouse peritoneal macrophages (PMφs)
Tissues Examined:
Lung, liver, spleen, kidney
Biochemical Markers:
ALT, AST, LDH, creatinine
Cytokines and Chemokines Measured:
Prokineticin-2, TNF-α, IL-6, IL-10, IL-17A
Experimental Interventions:
rPK2 stimulation, PKR1/PKR2 siRNA transfection, macrophage depletion with clodronate liposomes
Time Points of Analysis:
24 hours and 48 hours after modeling
Detection Methods:
Hematoxylin-eosin staining, fluorescence microscopy, FACScan flow cytometry, ELISA assays
Software for Analysis:
FCM Express
Referenced Studies:
Song et al., 2016; Song et al., 2015
Animal Age:
Six- to eight-week-old mice
Year:
2026
Research Institution:
Unspecified biomedical research laboratory
Type of Document:
Supplementary data for a scientific study
Subject:
NK-92 cell cytotoxicity, CA9, NHE1, gene expression profiling
Organism / Cell Line:
Human melanoma cell lines WM3629, WM1727A, WM4237; NK-92 and NK-92MI cells
Methods:
In vitro cytotoxicity assays, immunoblotting, confocal microscopy, mRNA sequencing
Key Genes / Proteins:
CA9, NHE1, c-Myc, SCO2, ITGB1P1, TRIM9, SVIL, KDM5B, S100A8, NTRK2, SMAD3, MYO1E, FMNL2, GABRB3, RBM41, P2RX5, PDGFRL
Experimental Conditions:
pH-controlled media, empty vector controls, constitutively active NHE1, NHE1-E262I mutant
Data Type:
Figures S1–S5, Tables S1–S2
Statistical Analysis:
Two-way ANOVA, unpaired t-test
Year:
2026
Topic:
Cellular biology, cancer research, ovarian failure
Document Type:
Research article
Author:
Rongli Wang, Xinyuan Yang
Target Audience:
Researchers, medical professionals
Period of Action:
N/A
Approval Date:
N/A
Modification Date:
N/A
Year:
2026
Region / City:
International (France, Belgium, UK, Germany, Finland)
Topic:
Drug-induced liver injury (DILI) assessment
Document Type:
Research article
Institution / Organization:
KaLy-Cell, UCB BioPharma, MRC Centre for Drug Safety Science, Sanofi-Aventis, GlaxoSmithKline, Orion Corporation, AstraZeneca, Université de Franche-Comté
Authors:
Lysiane Richert, Audrey Baze, Céline Parmentier, Helga Gerets, Rowena Sison-Young, Martina Dorau, Cerys Lovatt, Andreas Czich, Christopher Goldring, Kevin Park, Satu Juhila, Alison J. Foster, Dominic Williams
Funding:
IMI MIP-DILI project (Grant Agreement number 115336)
Target Audience:
Researchers in pharmacology and toxicology
Methodology:
Comparative in vitro cytotoxicity assays using 2D and 3D human hepatocyte cultures
Compounds Tested:
16 training compounds (12 DILI-positive, 4 non-DILI)
Exposure Periods:
2 hours (suspension), 3 days (2D-sw), 14 days (3D-MT co-culture)
Endpoints:
DILI prediction accuracy, sensitivity, and specificity
Abbreviations:
DILI, NPC, 2D-sw, SM, MT, TC
Note:
Year
Topic:
Respirable Crystalline Silica Program, Occupational Safety
Document Type:
Safety Program
Organization / Institution:
Penn State University
Author:
Penn State Environmental Health and Safety (EHS)
Target Audience:
Penn State employees, faculty, students, researchers, and affiliates
Effective Period:
Ongoing
Year:
2020
Jurisdiction:
Victoria, Australia
Document type:
Regulatory Impact Statement
Subject:
Occupational health and safety regulation
Focus:
Respirable crystalline silica exposure
Industry sectors:
Construction, manufacturing, stonemasonry, earth resources
Regulatory framework:
Occupational Health and Safety Act 2004
Proposed regulation:
Occupational Health and Safety Regulations 2017 amendments
Issuing body:
WorkSafe Victoria
Contributing authority:
Better Regulation Victoria
Consultation:
Stakeholder consultation undertaken
Health risks addressed:
Silicosis and silica-related diseases
Affected materials:
Engineered stone, stone, concrete, bricks, tiles
Relevant period:
2019–2021
Proposed commencement:
2021
Year:
2026
Region / City:
United States
Topic:
Occupational Health and Safety
Document Type:
Safety Program
Organization / Agency:
Occupational Safety and Health Administration (OSHA)
Target Audience:
Construction employees, project managers, safety personnel
Applicable Standard:
OSHA Respirable Crystalline Silica Construction Standard 29 CFR 1926.1153
Scope:
All construction work with potential exposure to respirable crystalline silica
Responsibilities:
Safety Department, Project Manager, Competent Person, Employees
Control Measures:
Exposure Control Plan (ECP), monitoring, PPE, training, medical surveillance
Year:
2023
Region / city:
Cincinnati, OH, USA
Topic:
Airborne dust, crystalline silica, laboratory testing, sampling efficiency
Document type:
Research article
Institution:
Centers for Disease Control and Prevention (CDC), National Institute for Occupational Safety and Health (NIOSH)
Author:
Drew Thompson, Chaolong Qi
Target audience:
Researchers, occupational health professionals
Date of approval:
N/A
Date of modifications:
N/A
Document type:
Supplementary information to a scientific research article
Scientific field:
Materials science; nanomaterials; electrochemistry
Research topic:
Low-temperature liquid exfoliation synthesis of few-layer β12-borophene sheets
Material studied:
β12-borophene sheets; boron
Methods:
Low-temperature liquid exfoliation (LTLE); SEM; UV–vis spectroscopy; XRD; EELS; HRTEM; DFT calculations
Solvents investigated:
N,N-Dimethylformamide (DMF); isopropyl alcohol (IPA); N-methyl-2-pyrrolidone (NMP)
Key measurements:
Sheet size distribution; extinction coefficient; UV–vis absorption spectra; XRD diffraction patterns; lattice structure; thickness distribution
Crystalline structure:
Few-layer single crystalline β12-borophene
Observed properties:
Metallic behavior; high dispersion and solubility in NMP
Figures referenced:
Supplementary Figures 1–5
Authors:
Lin et al.
Subject application:
Electrocatalysts for lithium–sulfur batteries
Year:
2026
Region / City:
Not specified
Subject:
Supramolecular chemistry and crystallography
Document type:
Original research article
Institution / Organization:
Not specified
Author:
Not specified
Target audience:
Researchers in chemistry and materials science
Methodology:
Experimental synthesis, X-ray crystallography, Density Functional Theory calculations
Chemical compounds:
Diisopropylammonium phenylsulfonate, diisopropylamine, phenylsulfonic acid
Solvent:
Ethanol
Analytical methods:
Crystallographic analysis, vibrational frequency calculations, HOMO-LUMO evaluation
Computational details:
DFT/B3LYP method with 6-311G(d,p), 6-311+G(d,p), 6-311++G(d,p) basis sets
Structural properties analyzed:
Bond lengths, bond angles, dipole moment, global reactivity descriptors
Crystallization period:
Approximately one month
Experimental conditions:
Room temperature, standard pressure (1 atm)