№ files_lp_4_process_3_111994
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Supplementary experimental figure descriptions presenting quantitative measurements of antigen uptake in murine bone marrow–derived dendritic cells, in vitro T cell proliferation after dendritic cell co-culture, and comparative outcomes in B16F10-OVA tumor-bearing mice with sarcosine-loaded dendritic cells.
Document type: Supplementary scientific figures
Field: Immunology
Research topic: Dendritic cells, antigen uptake, T cell proliferation, and sarcosine effects in tumor models
Experimental model: Murine bone marrow–derived dendritic cells (BM-DC) and B16F10-OVA tumor-bearing mice
Biological materials: FITC-OVA antigen, OVA-mRNA electroporated dendritic cells, sarcosine treated dendritic cells
Methods: Antigen uptake measurement by mean fluorescence intensity (MFI), in vitro T cell proliferation assay, co-culture experiments, tumor implantation and monitoring
Statistical analysis: Unpaired t test; two-way ANOVA
Sample size: n=3; n=5; n=10
Measured indicators: Antigen uptake (MFI), T cell proliferation percentage, body weight, tumor volume
Experimental comparison groups: Control BM-DC, untreated BM-DC with OVA-mRNA electroporation, sarcosine treated BM-DC with OVA-mRNA electroporation, sarcosine loaded tumor conditions
Organism: Mouse
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