№ lp_2_3_23990
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Educational case study outlining readings, conceptual explanations, and structured questions on caffeine chemistry, plant allelopathy, and the enzymatic and structural basis of caffeine biosynthesis for analysis in a university-level biology course.
Year:
2014
Discipline:
Plant biology; biochemistry; molecular biology
Document type:
Classroom case study and assignment
Authors:
Brian Sullivan; Shuchismita Dutta
Affiliations:
Department of Plant and Microbial Biology, North Carolina State University, Raleigh NJ 27695; Institute for Quantitative Biomedicine, Rutgers University, Piscataway NJ 08854
Contact author:
[email protected]
Related publications:
Science, 2014, 345, 1181–1184; The New York Times, September 2014
Key topics:
Caffeine biosynthesis; N-methyltransferases; allelopathy; enzyme structure; Protein Data Bank
Referenced organisms:
Coffee plant; tea plant; cocoa plant; mate plant
Referenced resources:
DrugBank; RCSB Protein Data Bank
Educational context:
Pre-class preparation and in-class discussion
Price: 8 / 10 USD
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The product description is provided for reference. Actual content and formatting may differ slightly.
Year:
2023
Region / city:
Singapore
Theme:
Protein translation, exopolysaccharide biosynthesis, stress response, quality control
Document type:
Research Project
Institution:
Nanyang Technological University
Author:
GAO Yonggui
Target audience:
Researchers, students, academics in molecular biology, biochemistry, and related fields
Duration:
Not specified
Approval date:
Not specified
Modification date:
Not specified
Year:
2026
Region / City:
Patras, Greece
Topic:
Bioethanol Production
Document Type:
Research Article
Organization / Institution:
University of Patras, Ionian University, Ulster University
Authors:
Archontoula Kalogeropoulou, Iris Plioni, Dimitra Dimitrellou, Magdalini Soupioni, Poonam Singh Nigam, Maria Kanellaki, Athnasios A. Koutinas
Target Audience:
Researchers, Environmental Scientists, Biotechnology Engineers
Period of Validity:
N/A
Date of Approval:
N/A
Date of Revisions:
N/A
Year:
2026
Region / City:
Not specified
Topic:
Triterpenoid biosynthesis, Plant UGTs
Document Type:
Scientific data table
Organization:
Not specified
Author:
Not specified
Target Audience:
Researchers in biochemistry, pharmacology, and plant sciences
Period of Action:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Year:
2023
Region / city:
Pasadena, CA
Topic:
PHA biosynthesis, microbial species, genetic analysis, polymer properties
Document type:
Research paper
Organization / institution:
NASA Jet Propulsion Laboratory
Author:
Wageesha Sharma, Dr. David Salem, Dr. Nitin Singh, Dr. Kasthuri Venkateswaran, Dr. Rajesh Sani
Target audience:
Researchers in the field of biotechnology, microbiology, and polymer science
Period of validity:
Not applicable
Approval date:
Not provided
Date of changes:
Not provided
Year:
2021
Topic:
Biosynthesis, Genetic Engineering, Phenazine-1-Carboxylic Acid
Document Type:
Research Article
Organization / Institution:
Qilu University of Technology (Shandong Academy of Sciences), Dezhou University
Author:
Kaiquan Liu, Zhenghua Li, Xiaoli Liang, Yanpeng Xu, Yufei Cao, Ruiming Wang, Piwu Li, Ling Li
Target Audience:
Researchers in microbiology, genetic engineering, and biochemistry
Period of Validity:
N/A
Approval Date:
2021
Date of Changes:
N/A
Contextual Description:
This research article presents the biosynthesis and genetic engineering techniques applied to produce phenazine-1-carboxylic acid in Pseudomonas chlororaphis Lzh-T5, focusing on gene modification methods for enhanced production.
Year:
2013
Region / City:
United States
Topic:
Chemistry, Health
Document Type:
Article
Organization:
Not specified
Author:
Paul Cowan
Target Audience:
General public, students
Effective Period:
Not specified
Approval Date:
Not specified
Modification Date:
Not specified
Year:
2023
Region / City:
Scotland
Topic:
Caffeine and Sugar Content in Beverages
Document Type:
Informational Guide
Organization:
Health Canada, World Health Organisation
Author:
Not specified
Target Audience:
Teenagers, General Public
Period of validity:
Not specified
Date of approval:
Not specified
Date of changes:
Not specified
Year:
2019
Region / city:
Australia, New Zealand
Theme:
Food safety
Document type:
Notification
Agency:
Food Standards Australia New Zealand (FSANZ)
Target audience:
WTO members, food industry
Period of validity:
From December 2019
Date of approval:
12 December 2019
Date of amendments:
N/A
Description:
The document informs about a temporary ban on the retail sale of foods containing high levels of caffeine in Australia and New Zealand, following health risks related to pure caffeine ingestion.
Year:
2026
Topic:
Nutrition / Health
Document Type:
Article compilation
Authors:
Multiple (unspecified)
Target Audience:
General public
Key Findings:
Conflicting conclusions on caffeine’s dehydrating effect; lethal dose information included
References:
Studies on coffee consumption and hydration
Keywords:
caffeine, dehydration, coffee, diuretic, fluid balance
Year:
2026
Region / City:
Not specified
Subject:
Chemistry / Organic Chemistry
Document Type:
Laboratory guide / Experiment protocol
Institution:
Educational laboratory / School science department
Author:
Not specified
Target Audience:
Students and laboratory technicians
Experiment Level:
Advanced Higher / Secondary education
Chemicals Used:
Caffeine tablets, sodium carbonate, anhydrous sodium sulfate, ethyl ethanoate, ethanol
Equipment:
Beakers, conical flasks, Bunsen burner, hotplate, separating funnel, filter funnels, Buchner/Hirsch funnel, ice bath, balance, stirrers, pipettes
Procedure Steps:
Extraction, recrystallization, TLC identification
Safety Precautions:
Flammable solvents, respiratory irritants, eye and mucous membrane protection
Duration:
Multiple sessions possible, including overnight recrystallization
Reference Compounds:
Caffeine from tablets or other sources (e.g., tea)
Year:
2026
Region / City:
Not specified
Topic:
Human physiology, sleep research, caffeine metabolism
Document Type:
Research supplement
Institution:
University or clinical research laboratory (not specified)
Authors:
Not specified
Study Population:
Healthy adults aged 18–35, BMI 18–25, non-shift workers
Exclusion Criteria:
Extreme chronotype, high/low caffeine intake, poor sleep quality, substance use, recent transmeridian travel
Laboratory Assessments:
Sleep efficiency, apnea index, periodic leg movements, salivary caffeine concentration
Data Collection Period:
Not specified
Ethical Compliance:
Screening and toxicology assessments performed
Year:
2026
Institution:
SNAB / Exam Board
Centre Number:
12416
Candidate Number:
Provided in document
Subject:
Biology
Type:
Core practical assessment
Topic:
Effect of caffeine on heart rate
Organ / Teacher:
Teachers’ initials provided in document
Practical Number:
1
Skills Assessed:
Experimental technique, variable control, data collection, analysis and interpretation
Safety Considerations:
Use of stroboscope for photosensitive individuals, ethical treatment of Daphnia
Equipment:
Culture of Daphnia, cavity slides, pipettes, distilled water, caffeine tablets, cotton wool, standard glassware, stopclock, paper towels, microscope
Year:
2023
Region / city:
European Union
Subject:
Food additives, food enzymes, food flavourings
Document type:
Practical guidance
Organization / institution:
European Commission, DG SANTE
Author:
European Commission
Target audience:
Applicants for updating Union lists of food additives, food enzymes, and food flavourings
Effective period:
Ongoing
Approval date:
7 July 2023
Date of changes:
7 July 2023
Research Theme:
Microbiology; Synthetic biology
PhD Research Project Title:
Genome-guided discovery of novel microbial enzymes and natural products
Scholarship category:
SBS Research Student Scholarship
Principal Investigator:
Liang Zhao-Xun
Co-supervisor/Collaborator:
NA
Proposed work:
Microbial genomics, Bioinformatics, Enzyme discovery, Functional characterization, Genetic manipulation, Synthetic biology
Preferred skills:
Basic understanding of biochemistry and molecular cloning
Application contact:
[email protected]
Application portal:
https://venus.wis.ntu.edu.sg/GOAL/OnlineApplicationModule/frmOnlineApplication.ASPX
Year:
2026
Region / city:
Deep-sea vent habitats
Subject:
Functional analysis of N-linked glycosylation systems
Document type:
Research article
Organization / institution:
London School of Hygiene and Tropical Medicine, University of Manchester, Cornell University
Author:
Dominic C Mills, Adrian J Jervis, Sherif Abouelhadid, Laura E Yates, Jon Cuccui, Dennis Linton, Brendan W Wren
Target audience:
Researchers in biochemistry and microbiology
Period of validity:
Ongoing research
Approval date:
N/A
Modification date:
N/A
Note:
Year
Year:
2022
Region / City:
Barcelona, Spain
Topic:
Genetic Engineering, Enzymes, Environment
Document Type:
Research Report
Organization:
FHNW, BSC, CSIC
Author:
Víctor Guallar, Manuel Ferrer
Target Audience:
Researchers, Environmental Scientists, Industry Professionals
Action Period:
From 01/08/2021
Date of Approval:
30/09/2022
Modification Date:
N/A
Work Package:
WP5, Enhancing enzymes through innovative engineering
Duration:
40 months
Lead Beneficiary:
FHNW
Participants:
FHNW, CSIC, BSC, Bangor, UHAM, UDUS, Inofea AG, Eucodis
Contact:
Víctor Guallar, [email protected]
Year:
2023
Region / City:
Los Angeles, NJ
Topic:
Biochemistry, Molecular Biology
Document Type:
Educational Lesson
Organization / Institution:
Los Angeles Mission College, Rutgers University
Author:
Brian Gadd, Shuchismita Dutta
Target Audience:
Students in molecular biology, biochemistry, or related fields
Period of Validity:
Ongoing
Approval Date:
N/A
Modification Date:
N/A
Year:
2026
Subject:
Biology / Biochemistry
Document Type:
Laboratory Manual
Institution:
OpenStax / Educational Laboratory
Audience:
Undergraduate Biology Students
Experiment:
Catalase Activity under Various Conditions
Techniques:
Spectrophotometry, Graph Construction, Experimental Design
Learning Objectives:
Define enzyme properties, analyze activity under conditions, conduct experiments, interpret data, report results
Year:
2026
Region / City:
Not specified
Topic:
Bariatric surgery, liver enzymes
Document type:
Research Supplementary Material
Institution:
Not specified
Author:
Not specified
Target audience:
Researchers, medical professionals
Period of validity:
Not specified
Approval date:
Not specified
Date of changes:
Not specified
Context:
A supplementary table providing quantification and allocation of inclusion indicators for abnormal elevation of liver enzymes in patients post-bariatric surgery.