№ files_lp_3_process_9_59916
File format: docx
Character count: 3157
File size: 29 KB
This document outlines a detailed surgical protocol for performing parabiosis in a mouse model to study shared physiological systems between two organisms.
Year:
2026
Region / City:
N/A
Theme:
Biological Research, Surgical Procedure
Document Type:
Protocol
Institution / Organization:
N/A
Author:
Mark Kelly LAT, Timothy P. Fitzgibbons MD PhD
Target Audience:
Laboratory Technicians, Researchers in Biological Sciences
Effective Period:
N/A
Approval Date:
N/A
Modification Date:
N/A
Price: 8 / 10 USD
The file will be delivered to the email address provided at checkout within 12 hours.
The file will be delivered to the email address provided at checkout within 12 hours.
Don’t have cryptocurrency yet?
You can still complete your purchase in a few minutes:- Buy Crypto in a trusted app (Coinbase, Kraken, Cash App or any similar service).
- In the app, tap Send.
- Select network, paste our wallet address.
- Send the exact amount shown above.
The final amount may vary slightly depending on the payment method.
The file will be sent to the email address provided at checkout within 24 hours.
The product description is provided for reference. Actual content and formatting may differ slightly.
Year:
2023
Region / City:
Australia
Theme:
Animal Welfare, Research Protocol
Document Type:
Research Guidance
Institution:
Australian National University (ANU)
Author:
ANU Veterinary Services
Target Audience:
Researchers, Animal Technicians, Veterinary Staff
Period of Validity:
Ongoing
Approval Date:
Not specified
Amendment Date:
Not specified
Year:
2023
Region / City:
Australia
Topic:
Animal Welfare, Research Protocol
Document Type:
Guidelines
Organization:
Australian National University
Author:
ANU Veterinary Services
Target Audience:
Researchers, Animal Technicians, Veterinary Staff
Period of Validity:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Context:
Guidelines for assessing the health and welfare of older mice in research using a standardized scoring system in line with ethical protocols.
Note:
Year
Subject:
Animal welfare, Pain assessment
Document type:
Template, Guidance
Institution:
ANU
Target audience:
Researchers, animal technicians, veterinary staff
Contextual description:
Guide for researchers to assess mouse health and welfare through a scoring system based on visible indicators of pain, distress, and overall condition.
Version:
V2.0
Institution:
Australian National University
Document type:
Animal welfare guidance
Species:
Mouse
Procedure:
Oral gavage
Assessment system:
Mouse Score System with Mouse Grimace Scale
Monitoring period:
Seven days post oral gavage
Scoring range:
0–3
Humane endpoints:
Defined by score criteria
Approval authority:
Animal Ethics Committee
Veterinary contact:
ANU Veterinary Services
Record keeping:
Cage card and relevant database
Interventions:
Supportive therapies and euthanasia criteria
Note:
Year
Year:
2023
Region / city:
Not specified
Subject:
Organelle isolation, immunoblotting, proteomic analysis
Document type:
Scientific protocol
Organization / institution:
Not specified
Author:
Matthew Taylor, Pui Yiu Lam, Francesca Tonelli, Dario Alessi
Target audience:
Researchers in molecular biology, cell biology, and proteomics
Period of validity:
Not specified
Date of approval:
Not specified
Date of amendments:
Not specified
Year:
2026
Dates:
February 2–6, 2026
Theme:
The Little Mouse, The Red Ripe Strawberry, and the Big Hungry Bear
Literacy Focus:
The Little Mouse, The Red Ripe Strawberry, and the Big Hungry Bear by Audrey Wood
Media Resource:
YouTube video reading (https://youtu.be/cELSeYq2auI
Note:
)
Songs and Fingerplays:
I Am a Strawberry (tune: I’m a Little Teapot)
Developmental Areas:
Literacy; Social Emotional; Language; Physical Development & Health
Vocabulary Words:
mouse; bear; strawberry; hidden; guarding; disguise
Standards Alignment:
Kansas State Standard PS Standard 4: Foundation Reading Skills
Preschool Learning and Development Expectation:
Shows understanding that print conveys meaning; demonstrates understanding of print directionality (left to right, top to bottom, front to back)
Learning Objectives:
Children explore objects using senses; respond to music; move body parts; build relationships with peers
Target Age Group:
Infants
Product code:
ab206987
Product name:
Mouse beta IG H3 (TGFBI) SimpleStep ELISA® Kit
Intended use:
Quantitative measurement of mouse beta IG H3 (TGFBI)
Analyte:
Mouse beta IG H3 (TGFBI) protein
Sample types:
Mouse plasma, cell culture supernatants, cell and tissue extracts
Application:
In vitro enzyme-linked immunosorbent assay (ELISA)
Format:
Pre-coated 96-well microplate (12 x 8 well strips)
Detection method:
Colorimetric detection at 450 nm or kinetic measurement at 600 nm
Storage conditions:
2–8ºC
Research use statement:
For research use only, not for diagnostic procedures
Kit components:
Capture antibody, detector antibody, recombinant protein standard, antibody diluent, wash buffer, cell extraction buffers, TMB development solution, stop solution, sample diluent, plate seal
Species reactivity:
Mouse
Protein characteristics:
683 amino acids, 75 kDa, contains EMI domain, four FAS1 domains, and one RGD motif
Assay principle:
Immunocapture using affinity tag labeled capture antibody and reporter conjugated detector antibody with HRP/TMB color development
Regulatory status:
Not intended for diagnostic use
Year:
2023
Organism:
Mus musculus
Tissue types:
Optic nerve, cerebellum, C2C12 cells
Techniques:
Cell lysis, transfection, RNA extraction, western blotting, immunofluorescence, RT-PCR
Reagents and tools:
RSB-100 buffer, TriZOL, Turbo DNase, Lipofectamine2000, plasmids (pcDNA3.1, tdTomato, Qk reporters), siRNAs, morpholinos, primary and secondary antibodies
Institution:
University of California, Santa Cruz
Animal model:
Adult wild-type 129-C57BL/6 mice
Ethics approval:
UCSC Institutional Animal Care and Use Committee
Target proteins:
QKI isoforms (QKI-5, QKI-6, QKI-7), GAPDH, Myc, HA, histone H3, tdTomato
Assays:
Dual Glo Luciferase Assay, BioAnalyzer quantitation, RT-qPCR
Method type:
Experimental protocol
Supplementary information:
Details on plasmid construction, antibody sources, and subcellular fractionation
Year:
2020
Region / City:
Rockefeller University, McGill University
Subject:
Mouse embryonic stem cell culture, histone modifications, chromatin immunoprecipitation sequencing
Document Type:
Research Methodology
Organization / Institution:
Rockefeller University, McGill University
Author:
Not specified
Target Audience:
Researchers in molecular biology and cell biology
Period of Action:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Context:
Methodology for culturing mouse ES cells, histone modification analysis, and chromatin immunoprecipitation sequencing techniques used in biological experiments.
Year:
2021
Region / City:
Zhejiang Province, China
Topic:
Epilepsy, Neuroscience
Document Type:
Research Article
Organization / Institution:
Zhejiang University
Author:
Lin Zhou, Liang Zhou, Li-Da Su, Sheng-Long Cao, Ya-Jun Xie, Ying Shen
Target Audience:
Researchers in neuroscience and epilepsy
Period of Action:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Year:
2
Region / City:
Not specified
Topic:
Child Development, Cognitive Learning
Document Type:
Guide Sheet
Organization / Institution:
Not specified
Author:
Laura Numeroff
Target Audience:
Parents, Caregivers, Early Childhood Educators
Effective Period:
Not specified
Approval Date:
Not specified
Modification Date:
Not specified
Year:
2026
Week:
February 2–6
Age Group:
Two-Year-Olds
Theme:
The Little Mouse the Red Ripe Strawberry and the Big Hungry Bear
Related Work:
The Little Mouse, the Red Ripe Strawberry, and the Big Hungry Bear
Author of Related Work:
Audrey Wood
Subject Areas:
Literacy; Physical Development & Health; Social/Emotional; Science; Language; Math; Social Studies
Letter of the Week:
e
Learning Objectives:
Listening skills during story and circle time; one-to-one correspondence counting 1–10; fine motor skills; vocabulary development
Vocabulary Focus:
author, illustrator, strawberry, ripe, mouse, bear, guarding, disguise, half, big, little, hidden, mile, pecked
Activities:
Story reading; Show-n-Share; Freeze Dancing; sequencing; counting; discussion of feelings; healthy foods; strawberry life cycle; animal homes matching
Standards Reference:
PS Standard 4: Foundation Reading Skills
Development Expectation:
Shows understanding that print conveys meaning; demonstrates understanding of print directionality
Year:
2018
Region / city:
N/A
Subject:
Neuroscience
Document type:
Supplementary figure
Organization:
N/A
Author:
D. Kroeger, G. Absi, C. Gagliardi, S. S. Bandaru, J. C. Madara, L. L. Ferrari, E. Arrigoni, H. Munzberg, T. E. Scammell, C. B. Saper, R. Vetrivelan
Target audience:
Researchers in neuroscience
Effective period:
N/A
Approval date:
N/A
Date of changes:
N/A
Document Type:
Supplemental figure legends
Field:
Molecular biology
Research Area:
Gastrointestinal epithelium and intestinal metaplasia
Subjects:
CDX2, HNF4A, SOX2, gastric organoids, Barrett’s esophagus, gastric intestinal metaplasia
Biological Material:
Human gastrointestinal tissues and mouse stomach organoids
Methods:
Single-cell RNA-seq, bulk RNA-seq, ATAC-seq, CUT&RUN, ChIP-seq, GSEA, enhancer analysis, immunohistochemistry, qRT-PCR
Referenced Studies:
Busslinger et al. 2021b; Nowicki-Osuch et al. 2021; Owen et al. 2018; Companioni et al. 2017; Singh et al. 2021; Gao and Kaestner 2010; Wang et al. 2013
Key Topics:
Transcriptional profiling, chromatin accessibility, enhancer activity, transcription factor binding, intestinalization of gastric epithelium
Species:
Homo sapiens; Mus musculus
Pathological Context:
Barrett’s esophagus; gastric intestinal metaplasia
Year:
2023
Region / City:
United States
Subject:
Commercial Sales Practices
Document Type:
Instructional Guidelines
Agency / Organization:
Government
Author:
Unknown
Target Audience:
Contractors
Effective Period:
Ongoing
Approval Date:
Unknown
Date of Changes:
Unknown