№ files_lp_4_process_3_073860
Year: 2014–2018
Region / City: Not specified
Subject: Breast cancer, tumor-infiltrating lymphocytes, NFκB signaling
Document Type: Supplementary Methods and Figures
Institution: International TILs Working Group; contributing research laboratories
Authors: Salgado R, Denkert C, Demaria S, Sirtaine N, Klauschen F, Pruneri G, et al.
Target Audience: Cancer researchers, immunologists, molecular biologists
Study Period: Before and after Tamoxifen treatment
Sample Size: 64 pre-treatment, 39 post-treatment for TIL analysis; 15 patients for multiplex immunofluorescence; 13 paired samples for immune subset analysis
Methods: H&E tumor sections, multiplex immunofluorescence, RT-QPCR, Western Blot, clonogenic assays, GFP reporter assays
Key Variables: Tumor-infiltrating lymphocyte percentage, expression of NFκB-target genes, EMT/stemness factors, p65 and p50 protein levels
Supplementary Figures: 1–6
References: 1–10
Experimental Models: T47D and MCF-7 breast cancer cell lines, CRISPR/Cas9 knockouts
Price: 8 / 10 USD
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