№ lp_1_2_39427
File format: docx
Character count: 3211
File size: 21 KB
Academic cover sheet and quality control checklist accompanying a student-submitted actinobacteriophage genome annotation, summarizing annotation decisions, validation checks, and noted problem areas for reviewer assessment.
Phage name:
BenchScraper
Document type:
Genome annotation submission cover sheet
Subject area:
Actinobacteriophage genomics
Institution:
Southeastern Louisiana University
Author:
Justin Anderson
Primary email:
[email protected]
Correspondence email:
[email protected]
Quality control process:
SEA-PHAGES genome annotation QC review
Highlighted gene:
Gene 53
Annotation issues noted:
Integrase classification, HTH DNA-binding assignment, tRNA prediction confidence
Annotation tools mentioned:
DNA Master, PECAAN, Aragorn, tRNAscan-SE
Documentation methods:
PECAAN output, DNA Master shorthand, spreadsheet
External documentation link:
Google Sheets annotation log
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The product description is provided for reference. Actual content and formatting may differ slightly.
Phage name:
Sorpresa
Document type:
Genome annotation submission cover sheet
Scientific field:
Actinobacteriophage genomics
Institution:
Brigham Young University
Author:
Jayden S. Longhurst
Contributor roles:
Student annotator
Quality control context:
SEA-PHAGES genome annotation review
Genes highlighted for review:
ORF 90, ORF 68
Genome features discussed:
Orphams, transposase, synteny rearrangement
Annotation tools referenced:
DNA Master, PECAAN, Aragorn, BLAST, PhagesDB
Submission purpose:
Quality control review of genome annotation
Source type:
Academic course-related scientific documentation
Year:
2023
Topic:
Phage Naming
Document Type:
Guidelines
Institution:
SEA-PHAGES
Target Audience:
Students, Researchers
Effective Period:
Ongoing
Approval Date:
Not specified
Changes Date:
Not specified
Year:
2026
Institution:
University of Mary Washington
Location:
Fredericksburg, VA, USA
Event:
8th Annual SEA-PHAGES Symposium
Document Type:
Abstract
Corresponding Faculty Member:
Lynn Lewis ([email protected]
Note:
)
Authors:
Alexis N Robinson, Meredith D Snyder, Julia C Arrington, Rachel G Bellamy, Olivia C Blake, Robert C Byles, Samuel J Clark, Emily R Contompasis, Jacob N Ducey, Claire S Goode, Jenifer M Grove, Aidan M Hyland-Wood, Lynn O Lewis, Marion W Punches, Anna M Rinko, Sabrina L Sherman, Matthew A Tovar, Andrea G Zamora
Research Focus:
Isolation and genomic characterization of Bacillus phages
Phage Hosts:
Bacillus thuringiensis subsp. Kurstaki and Berliner
Number of Phages Isolated:
19
Sequenced Phages:
4
Notable Findings:
Identification of Di-ildio as potentially the largest Bacillus siphovirus discovered
Field:
Microbiology
Topic:
Bacteriophage quantification
Document type:
Laboratory protocol
Method:
Phage titer determination
Techniques described:
Full Plate Titer; Quick and Dirty Titer; Serial dilution; Spot test
Measured parameter:
Plaque Forming Units per milliliter (PFU/mL)
Host organism:
Mycobacterium smegmatis
Incubation temperature:
37 °C
Required materials:
Agar plates; MBTA; 7H9 medium with CaCl₂; phage buffer; micropipettes; serological pipettes; incubator
Experimental components:
Top agar plating mixture; phage lysate; dilution series (10⁻¹–10⁻¹⁰)
Observation method:
Plaque counting on agar plates
Recommended plaque range for calculation:
20–200 plaques
Output value:
Phage titer expressed in PFU/mL
Year:
2026
Organism:
Phage MisoSoup
Gene:
80
Protein type:
Amino acid sequence
Sequence length:
250 amino acids
Analysis method:
HHPRED
Matched protein:
S-adenosylmethionine synthase
Match probability:
100%
E-value:
0
Data type:
Molecular biology sequence
Year:
2026
Region / City:
Virginia, USA
Topic:
Genome Annotation
Document Type:
Cover Sheet
Organization / Institution:
Virginia Western Community College
Author:
Heather Lindberg
Target Audience:
QC reviewers, students
Period of Validity:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Year:
2023
Region / city:
Montclair, NJ
Subject:
Genomics, Phage Research
Document Type:
Submission Form
Institution:
Montclair State University
Author:
Kirsten Monsen-Collar
Target Audience:
Researchers, QC reviewers, Phage annotation teams
Period of Action:
Not specified
Approval Date:
Not specified
Modification Date:
Not specified
Year:
2023
Region / City:
Not specified
Theme:
Genome annotation, Actinobacteriophage
Document Type:
Cover Sheet
Organization / Institution:
CCSNH-NHTI
Author:
Joseph Christiansen, Beth Wilkes
Target Audience:
QC reviewers, annotators
Period of Validity:
Not specified
Approval Date:
Not specified
Amendment Date:
Not specified
Year:
2021
Region / City:
United States
Topic:
Phage genome annotation
Document type:
Submission cover sheet
Organization:
Nova Southeastern University
Author:
Ashley Janke
Target Audience:
QC reviewers, phage researchers
Date of submission:
Not specified
Date of modification:
Not specified
Year:
Not specified
Region / City:
Not specified
Subject:
Phage genome annotation
Document Type:
Cover sheet
Organization / Institution:
Virginia Western Community College
Author:
Heather Lindberg
Target Audience:
QC reviewer
Period of validity:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Year:
2023
Region / City:
Coastal Georgia
Topic:
Phage Genome Annotation
Document Type:
Cover Sheet
Organization / Institution:
College of Coastal Georgia
Author:
Holly Nance
Target Audience:
QC reviewers, researchers in genomics
Effective Period:
N/A
Approval Date:
N/A
Date of Changes:
N/A
Phage Name:
Schism
Author:
Justin Anderson
Institution:
Southeastern Louisiana University
Email:
[email protected]
Additional Emails:
[email protected]
Document Type:
Genome annotation submission cover sheet
Target Audience:
QC reviewers and instructors
Content:
Description of annotation process, gene issues, and validation checks
Gene Highlights:
Gene at stop 31170, Gene at stop 35089
File Type Submitted:
Spreadsheet
Protocols Followed:
tRNA annotation, frameshift annotation, Feature Table documentation
Validation Checklist:
DNA Master validation, LocusTag sequence, Feature_Type correctness, Product field verification
Year:
2023
Region / City:
Amherst, Massachusetts
Topic:
Genome Annotation
Document Type:
Cover Sheet
Organization / Institution:
UMass Amherst
Author:
Jessica Rocheleau
Target Audience:
QC reviewers
Period of Validity:
N/A
Date of Approval:
N/A
Date of Changes:
N/A
Phage name:
Sorpresa
Document type:
Genome annotation submission cover sheet
Scientific field:
Actinobacteriophage genomics
Institution:
Brigham Young University
Author:
Jayden S. Longhurst
Contributor roles:
Student annotator
Quality control context:
SEA-PHAGES genome annotation review
Genes highlighted for review:
ORF 90, ORF 68
Genome features discussed:
Orphams, transposase, synteny rearrangement
Annotation tools referenced:
DNA Master, PECAAN, Aragorn, BLAST, PhagesDB
Submission purpose:
Quality control review of genome annotation
Source type:
Academic course-related scientific documentation
Note:
Year
Author:
Tamarah Adair
Note:
Year
Author:
Kari Clase
Year:
2023
Region / City:
Poughkeepsie, NY
Topic:
Actinobacteriophage Genome Annotation
Document Type:
Genome Annotation Cover Sheet
Organization / Institution:
Marist College
Author:
Elizabeth Godin
Target Audience:
Students, QC reviewers
Action Period:
N/A
Approval Date:
N/A
Modification Date:
N/A