№ files_lp_4_process_2_88693
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Laboratory protocol describing procedures for determining bacteriophage concentration through plaque formation assays using serial dilution, agar plating, and plaque counting methods in Mycobacterium smegmatis cultures.
Field: Microbiology
Topic: Bacteriophage quantification
Document type: Laboratory protocol
Method: Phage titer determination
Techniques described: Full Plate Titer; Quick and Dirty Titer; Serial dilution; Spot test
Measured parameter: Plaque Forming Units per milliliter (PFU/mL)
Host organism: Mycobacterium smegmatis
Incubation temperature: 37 °C
Required materials: Agar plates; MBTA; 7H9 medium with CaCl₂; phage buffer; micropipettes; serological pipettes; incubator
Experimental components: Top agar plating mixture; phage lysate; dilution series (10⁻¹–10⁻¹⁰)
Observation method: Plaque counting on agar plates
Recommended plaque range for calculation: 20–200 plaques
Output value: Phage titer expressed in PFU/mL
Price: 8 / 10 USD
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