№ lp_1_2_49575
Laboratory procedural guidance describing the treatment of red blood cells with AET to inactivate Kell antigens and interpret serologic reactivity patterns in antibody investigations.
Document type: Laboratory procedure
Subject area: Immunohematology
Focus antigens: Kell system antigens and selected HTLA antigens
Chemical agent: 2-aminoethylisothiouronium bromide (6% AET)
Specimen type: EDTA anticoagulated whole blood
Materials and equipment: Cell washer, serological centrifuge, microscope, water bath or heating block at 37°C
Reagents: Phosphate buffered saline, 0.9% saline, anti-IgG, IgG-coated cells, anti-K or anti-k
Quality control: Verification of Kell antigen destruction using known anti-K or anti-k
Incubation conditions: 37°C for 20 minutes
Storage conditions: Treated cells stored in Alsevers solution for up to 7 days
Test methods: Antiglobulin testing, LISS-LIDAT methods
Interpretation framework: Comparative reactivity of treated versus untreated red cells
Document number: SP.009
Revision date: September 1, 2014
References: Judd’s Methods in Immunohematology; AABB Technical Manual, 17th edition
Price: 8 / 10 USD
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