№ files_lp_4_process_2_89823
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Detailed laboratory protocol for extracting and purifying DNA from lichen specimens and other fungi, including preparation of raw DNA extracts and PCR product purification using low-cost silica spin columns and custom buffers.
Year:
2012-2014
Region / location:
New Zealand (Dunedin, Campbell Island, Catlins, Greymouth, Southland), Tasmania
Subject:
Molecular biology, DNA extraction, PCR purification
Document type:
Research protocol
Institution:
University of Otago, Department of Botany
Author:
Summerfield T. C.
Target audience:
Researchers in molecular biology and mycology
Methods referenced:
Cubero et al. (1999), Summerfield (2003), Ye et al. (2004)
Specimen details:
Voucher specimens with collection dates and locations, held at Otago Regional Herbarium and Department of Conservation
Materials:
EconoSpin® All-In-One Mini Spin Columns, homemade buffers (PEX, WS, EB)
Procedures included:
Sample preparation, tissue disruption, cell lysis, chloroform extraction, spin-column purification of DNA and PCR products
Equipment:
Microcentrifuge, NanoDrop 1000 Spectrophotometer
Reference genome type:
Genomic DNA from lichens and other fungi
Price: 8 / 10 USD
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The product description is provided for reference. Actual content and formatting may differ slightly.
Note:
Year
Theme:
Catering / Menu
Document Type:
Menu
Contextual Description:
Catering menu offering a variety of hot and cold tapas, gluten-free, vegan, and vegetarian options, along with desserts.
Year:
2026
Region / City:
Not specified
Subject:
Solar energy, renewable energy experiments
Document Type:
Project report
Institution / Organization:
Not specified
Author:
Not specified
Target Audience:
Students, hobbyists, science enthusiasts
Period of Activity:
2026
Results:
Successful construction and measurement of a homemade solar cell, recommendations for future studies
Topic:
Food culture and eating habits
Type of document:
Analytical essay
Country / Region:
Vietnam
Subject:
Fast food consumption and homemade meals
Context:
Urban lifestyle and modern work pace
Time period discussed:
21st century
Key themes:
Fast food industry growth, household cooking practices, urbanization, lifestyle changes
Main focus:
Potential replacement of traditional home cooking by fast food
Mentioned social groups:
Adults, youth, housewives, cooking hobbyists
Language of document:
English
Note:
Year
Document Type:
Guidance
Organization / Institution:
BACP
Year:
2016
Region / city:
Sydney
Subject:
DNA/RNA extraction, laboratory safety
Document type:
Standard Operating Procedure
Organization:
The University of Sydney
Author:
Coleman, N.; Nikolic, A.
Target audience:
Laboratory personnel, researchers
Period of validity:
Ongoing
Approval date:
Not specified
Modification date:
Not specified
Year:
2023
Region / City:
Les Ulis, France
Topic:
Paper formatting guidelines
Document Type:
Guidelines
Institution:
EDP Sciences
Author:
Solange Guéhot, Isabelle Houlbert, Agnès Henri
Target Audience:
Authors submitting papers for conference proceedings
Period of Application:
Indefinite
Approval Date:
N/A
Date of Changes:
N/A
Date:
7/14/25
Subject:
Fiber reinforced polymer composite strengthening of concrete members
Type of document:
Technical specification
Work category:
Bridge construction and rehabilitation
Materials referenced:
Carbon fiber reinforced polymer (CFRP), glass fiber reinforced polymer (GFRP), epoxy resin, protective coating
Standards referenced:
ASTM D3039, NCHRP Report 678
Professional requirements:
Shop drawings and calculations signed and sealed by a Missouri Professional Engineer
Contractor requirements:
Minimum 5 years documented experience or 25 similar applications; manufacturer certification required
Design applications:
Concrete beam shear strengthening, precast octagonal pile confinement, column confinement, seismic axial resistance
Construction requirements:
Surface preparation, moisture testing, FRP wrapping procedures, anchorage and overlap provisions
Service life requirement:
50 years for protective coating
Organization:
Missouri Department of Social Services, Children’s Division
Document Type:
Assessment Tool
Date:
Not specified
Purpose:
Evaluating child safety and family situation
Participants:
Not specified
Rating Scale:
0–10 for child safety
Sections:
Concerns, Strengths, Required Actions, Case Specific Scaling Question
Intended Audience:
Child welfare professionals
Usage Context:
Case evaluation and decision-making regarding child safety
Year:
2019
Authors:
Henning Großkopf, Sarah Vogel, Claudia Damaris Müller, Sebastian Köhling, Jan-Niklas Dürig, Stephanie Möller, Matthias Schnabelrauch, Jörg Rademann, Ute Hempel, Martin von Bergen, Kristin Schubert
Type:
Research Article
Keywords:
glycosaminoglycans, proteins, mass spectrometry, affinity purification, hyaluronan, biotinylation
Region / City:
Not specified
Target Audience:
Scientists, researchers in biochemistry, molecular biology, and related fields
Research Focus:
Protein interactions with glycosaminoglycans
Methodology:
Mass spectrometry, affinity purification
Date of Publication:
2019
Supporting Materials:
Supplementary methods and materials provided
Year:
2018
Region / City:
Syracuse, NY
Subject:
Reverse Osmosis Water Purification System for Veterans Affairs
Document Type:
Solicitation
Agency:
Department of Veterans Affairs
Target Audience:
Small Business Suppliers
Period of Validity:
30 days after award
Approval Date:
06-12-2018
Modification Date:
N/A
Year:
2021
Region / City:
Johannesburg
Topic:
Water purification
Document Type:
Instruction Manual
Organization:
Home of Living Brands
Author:
Russell Hobbs
Target Audience:
Consumers of Russell Hobbs products
Period of Validity:
2 years
Approval Date:
2021
Date of Changes:
Not specified
Warranty Period:
2 years
Extended Warranty:
1 year (upon registration)
Description:
Instruction manual for the Russell Hobbs Purification and Mineralization Bottle, detailing features, assembly, usage instructions, and warranty conditions.
Year:
2023
Region / City:
Gainesville, FL
Topic:
DNA extraction and purification protocols
Document Type:
Protocol
Organization / Institution:
UF-IFAS Plant Diagnostic Center
Author:
Dr. Pei-Ling Yu
Target Audience:
Researchers in genomics and plant diagnostics
Effective Period:
January 2023
Approval Date:
January 12, 2023
Modification Date:
N/A
Subject:
Chemistry
Topic:
Organic compound extraction and purification
Document type:
Laboratory experiment procedure
Chemical compound:
Paracetamol
Experimental technique:
Extraction and recrystallisation
Educational level:
Secondary or introductory laboratory chemistry
Required chemicals:
Paracetamol tablets; propanone; water
Laboratory equipment:
Conical flasks; measuring cylinder; warm water bath; filter funnel; filter papers; beaker; pipette; cotton wool; glass funnel; Bunsen burner; tripod and gauze; melting point apparatus; balance
Safety requirements:
Eye protection; handling precautions for propanone vapour and flammable liquids
Process steps:
Dissolution in propanone; filtration; solvent evaporation; recrystallisation from water; crystallisation and drying
Analytical method:
Melting point determination
Key concept:
Effect of impurities on melting point and crystal structure
Year:
2026
Institution:
University of Latvia, Institute of Chemical Physics
Location:
Riga, Latvia
Authors:
Assoc. Prof. Elīna Pajuste, Mg. chem. Patrīcija Kalniņa, Mg. chem. Edgars Mamis
Type of document:
Research project description
Funding:
Latvian Council of Science, University of Latvia Foundation
Target radionuclides:
149Tb, 152Tb, 155Tb, Sc isotopes
Methods:
Mass separation, electrochemical and chemical purification, ion exchange resins, SEM, EDX, BET, FT-IR, pycnometry
Materials:
TaC, VC, VB, TiC-nanoscale, composite targets
Applications:
Theranostic radiopharmaceutical development, pre-clinical studies
Research facilities:
CERN-MEDICIS, University of Latvia radiochemistry lab
Year:
2021
Region / State:
Minnesota
Country:
United States
Topic:
Drinking water purification and pollutant monitoring
Document Type:
Research funding proposal
Funding Program:
Environment and Natural Resources Trust Fund Request for Proposal
Proposal ID:
2021-317
Project Title:
Sensor-Embedded Purification System for Clean Drinking Water
Author:
Tianhong Cui
Organization:
University of Minnesota – College of Science and Engineering
Project Manager:
Tianhong Cui
Contact Telephone:
(612) 626-1636
Contact Email:
[email protected]
Funding Category:
Water Resources (B)
Requested Funding:
$536,000
Proposed Project Completion Date:
2024-06-30
Project Location Scale:
Statewide
Impact Area:
Statewide
Impact Timing:
During the project and in the future
Key Technologies:
embedded sensors, activated carbon filtration, UV purification, multilayer conductive purification film
Target Contaminants:
lead, arsenic, cadmium, mercury, chloride, microbes, organic compounds
Planned Activities:
sensor array development, compact purification system construction, system integration and optimization
Activity Budget Allocation:
$356,000 (Activity 1); $180,000 (Activity 2)
Year:
2023
Region / City:
Prague, Czech Republic
Subject:
Water purification, micromotors
Document type:
Supplementary Information
Organization / Institution:
University of Chemistry and Technology Prague
Authors:
Huaijuan Zhou, Bing Wu, Lukas Dekanovsky, Shuangying Wei, Bahareh Khezri, Tomas Hartman, Jinhua Li, Zdenek Sofer
Target audience:
Researchers in chemistry, materials science, and environmental engineering
Period of validity:
Not specified
Approval date:
Not specified
Modification date:
Not specified
Context:
This document presents supplementary information related to a study on the integration of BiOI nanosheets into bubble-propelled micromotors for enhanced water purification efficiency, including experimental data and figures.
Year:
2023
Author:
Massimiliano Simons
Institution:
KU Leuven
Field:
Philosophy of Science / Science and Technology Studies
Document Type:
Academic Article
Keywords:
Bachelard, phénoménotechnique, epistemological rupture, purification, proliferation, science studies, Latour, Althusser
Audience:
Scholars and students in philosophy of science and STS
Abstract:
Analyzes the relationship between Bachelard’s concepts of epistemological break and phénoménotechnique, exploring historical interpretations and contemporary relevance
Period Covered:
1960s–1980s (historical uses), theoretical discussion up to 2023