№ files_lp_3_process_7_052912
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File size: 17 KB
This document lists various plasmids and their genotypes used in bacterial genetic research, with references to multiple studies in the field.
Year:
1999
Note:
Region / city
Topic:
Bacterial genetics, plasmids
Document type:
Research paper
Author:
Weiss et al., 1999; Beaupré et al., 1997; Datsenko and Wanner, 2000; Kang et al., 2005; Roggiani and Goulian, 2015
Target audience:
Researchers in molecular biology and genetics
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The product description is provided for reference. Actual content and formatting may differ slightly.
Year:
2016
Region / city:
N/A
Topic:
Molecular biology
Document type:
Table
Organization / institution:
N/A
Author:
Hugonnet et al., 2016; Morè et al., 2018; Banzhaf et al., 2012; Meisel et al., 2003; Meiresonne et al., 2017
Target audience:
Researchers in molecular biology and genetics
Period of validity:
N/A
Approval date:
N/A
Date of changes:
N/A
Year:
2016
Region / City:
-
Subject:
Genetic research
Document Type:
Research Supplementary Table
Author:
Wang et al.
Target Audience:
Researchers in molecular biology and microbiology
Period of validity:
-
Approval Date:
-
Date of Changes:
-
Context:
Supplementary data for a study on Vibrio alginolyticus strains and plasmids, including genetic modifications and relevant references.
Year:
2023
Region / City:
Not specified
Topic:
Microbiology, Genetic Engineering
Document Type:
Supplementary Information
Organization / Institution:
Not specified
Author:
Wolf M, Geczi A, Simon O, Borriss R
Target Audience:
Researchers in microbiology and genetic engineering
Effective Period:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Year:
2021
Region / city:
Not specified
Topic:
Bacterial strains, plasmids, gene replacement
Document type:
Supplementary material
Organization / institution:
Not specified
Author:
Not specified
Target audience:
Researchers in microbiology, molecular biology
Period of validity:
Not specified
Approval date:
Not specified
Date of changes:
Not specified
Year:
2023
Region / City:
Not specified
Subject:
Molecular biology, plasmids
Document type:
Research data
Institution:
Not specified
Author:
Not specified
Target audience:
Researchers in molecular biology and related fields
Period of validity:
Not specified
Approval date:
Not specified
Date of changes:
Not specified
Year:
2026
Region / City:
Not specified
Topic:
Molecular biology, gene manipulation
Document Type:
Research Supplementary Material
Organization / Institution:
Not specified
Author:
Not specified
Target Audience:
Researchers in molecular genetics and microbiology
Period of Validity:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Year:
2023
Region / City:
N/A
Topic:
Microbiology / Genetics
Document Type:
Supplementary Table
Organization / Institution:
N/A
Author:
N/A
Target Audience:
Researchers in microbiology and genetics
Period of Validity:
N/A
Approval Date:
N/A
Modification Date:
N/A
Keywords:
Bacterial strains, plasmids, genetic engineering, E. coli, Sinorhizobium meliloti, recombinant proteins
Context:
Supplementary table listing bacterial strains and plasmids used in a study on genetic engineering.
Year:
1995
Organism:
Escherichia coli, Bacillus subtilis
Document type:
Supplementary material
Source or reference:
Wolf et al., 1995; This work
Strain information:
Genotypes and modifications of bacterial strains including deletions and plasmid constructs
Plasmid information:
Description of plasmids with relevant genes and selectable markers
Primer information:
Sequences of primers used for gene modification and verification
Experimental focus:
Genetic characterization and construction of bacterial strains
References:
Wolf M, Geczi A, Simon O, Borriss R. Genes encoding xylan and beta-glucan hydrolysing enzymes in Bacillus subtilis: characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase. Microbiology 141 (Pt 2):281-90
Year:
2021
Region / City:
United Kingdom
Subject:
Health economics, antimicrobial evaluation
Document type:
Final report
Organization / Institution:
Centre for Health Economics, University of York; Health Economics and Decision Science, University of Sheffield
Author:
Sue Harnan, Ben Kearns, Alison Scope, Laetitia Schmitt, Dina Jankovic, Jean Hamilton, Ruth Wong, Tushar Srivastava, Harry Hill, Chu Chang Ku, Kate Ren, Claire Rothery, Laura Bojke, Mark Sculpher, Beth Woods
Target audience:
Healthcare professionals, policymakers, researchers
Period of validity:
2016 - ongoing
Approval date:
October 2021
Date of last modification:
October 2021
Document type:
Supplementary material
Scientific field:
Molecular biology
Research focus:
Anticalin selection and affinity maturation
Target protein:
Membrane-bound Hsp70
Methodology:
Bacterial surface display
Techniques:
FACS screening, affinity maturation, directed evolution, SPR, CD spectroscopy, flow cytometry
Biological models:
E. coli, FaDu cells, OPM-2 cells
Authors:
Lars Friedrich; Petra Kornberger; Claudia T. Mendler; Gabriele Multhoff; Markus Schwaiger; Arne Skerra
Figures included:
Figures S1–S7
Related publication:
Main research article on Anticalin selection against Hsp70
Experimental scope:
In vitro binding analysis and cellular characterization
Document type:
Laboratory exercise instructions
Subject area:
Microbiology
Educational level:
Undergraduate laboratory
Focus:
Bacterial growth media and culturing techniques
Topics covered:
Normal flora, aseptic technique, selective and differential media, colony morphology, hemolysis
Media discussed:
TSY agar, MacConkey’s agar, Mannitol Salt agar, Blood agar
Biological concepts:
Normal flora, bacterial colonies, hemolysis, Gram-positive and Gram-negative bacteria
Laboratory context:
Teaching laboratory
Associated organisms:
Staphylococcus, Streptococcus, Enterobacteriaceae
Intended audience:
Students enrolled in a microbiology laboratory course
Temporal context:
Multi-week laboratory sequence
Instructional purpose:
Observation and analysis of bacterial growth on culture media
Year:
2023
Region / City:
Not specified
Topic:
Microbiology, Genetic Engineering
Document Type:
Supplementary Information
Organization / Institution:
Not specified
Author:
Wolf M, Geczi A, Simon O, Borriss R
Target Audience:
Researchers in microbiology and genetic engineering
Effective Period:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Year:
2026
Institution:
University of Leicester
College:
College of Life Sciences
School/Department:
LeMID
Funding:
CLS / HPRU Grant studentship
First Supervisor:
Prof Julie Morrissey
Second Supervisor:
Dr Emma Marczylo, UK Health Security Agency (UKHSA)
Additional Supervisors:
Kate Jones, Health and Safety Executive; Emma-Jane Goode
Project Type:
PhD research project
Field:
Microbiology / Public Health
Focus:
Metal exposure, bacterial persistence, antimicrobial resistance
Target Organisms:
Staphylococcus aureus (CA-MRSA and HA-MRSA)
Training Provided:
Molecular microbiology, tissue culture, omics, microscopy
Collaborating Organizations:
Health Protection Research Unit, National Institute of Health Research (NIHR), Health and Safety Executive, UKHSA
Note:
Year
Topic:
Blood culture, bacterial infection, microbiology
Document type:
Procedure
Target audience:
Medical professionals, laboratory technicians
Year:
2023
Region / City:
Salt Lake City, Utah, United States
Subject:
Bacterial diversity, chemical ecology, natural products
Document Type:
Scientific Research Paper
Organization / Institution:
University of Utah, Scripps Institution of Oceanography, University of California at San Diego
Author:
Elijah R. Bring Horvath, William J. Brazelton, Min Cheol Kim, Reiko Cullum, Matthew A. Mulvey, William Fenical, Jaclyn M. Winter
Target Audience:
Researchers in microbiology, marine biology, and chemical ecology
Period of Validity:
N/A
Approval Date:
N/A
Date of Changes:
N/A
Year:
2018
Region / City:
Not specified
Topic:
Gene expression in biofilm and planktonic bacteria
Document type:
Scientific article
Organization / Institution:
Not specified
Author:
Not specified
Target audience:
Researchers in microbiology and molecular biology
Effective period:
Not specified
Approval date:
Not specified
Modification date:
Not specified
Document type:
Supporting information
Related article title:
Organic fertilizers shape the bacterial communities harboring pqqC and phoD genes by altering organic acids, leading to improved phosphorus utilization
Authors:
Liying Zhi; Bangxiao Zheng; Yunjie Xu; Jiayang Xu; Josep Peñuelas; Jordi Sardans; Yixiao Chang; Shuquan Jin; Hong Ying; Kai Ding
Affiliations:
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China; College of JunCao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, China; School of Environmental Science and Engineering, Xiamen University of Technology, Xiamen, China; Bureau of Agriculture and Rural Affairs, Wencheng, China; University of Chinese Academy of Sciences, Beijing, China; CSIC, Global Ecology Unit, CREAF-CSIC-UAB, Bellaterra, Spain; CREAF, Cerdanyola del Vallès, Spain; Institute of Ecological Environment, Ningbo Academy of Agricultural Sciences, Ningbo, China; Yangtze Delta Region Healthy Agricultural Institute (Zhejiang) Co., Ltd, Tongxiang, China
Number of pages:
12
Number of figures:
9
Number of tables:
5
Subject area:
Soil microbiology
Keywords:
organic fertilizer; inorganic fertilizer; pqqC gene; phoD gene; bacterial community; soil physicochemical properties; organic acids; phosphorus activation rate
Experimental design:
Fertilizer treatment groups including chemical and organic amendments
Analytical methods:
PCOA based on Bray-Curtis dissimilarity; Spearman correlation analysis; quantitative PCR; soil physicochemical and enzyme assays
Gene targets:
16S rRNA; pqqC; phoD
Measured parameters:
Soil pH; SOC; TN; TP; TK; AN; AP; AK; organic acid content; enzyme activities (ACP, ALP)
Year:
2006
Region / city:
Los Angeles, California
Subject:
Water quality, bacteria standards
Document type:
Amendment
Authority / institution:
Regional Water Quality Control Board, State Water Resources Control Board, U.S. Environmental Protection Agency
Author:
Not specified
Target audience:
Environmental professionals, policymakers, water quality regulators
Effective period:
June 8, 2006, and subsequent revisions
Approval date:
June 8, 2006
Revision date:
June 7, 2012, and later revisions
Context:
This document presents the revision of the Total Maximum Daily Load (TMDL) for bacterial indicator densities in water bodies in the Los Angeles region, with specific provisions for different water types and recreation uses.
Year:
Not specified
Region / City:
Not specified
Subject:
Bacterial meningitis diagnosis
Document type:
Laboratory protocol / Standard operating procedure
Institution:
Not specified
Target audience:
Laboratory personnel / medical staff
Specimen type:
Cerebrospinal fluid
Methods included:
Cell counts, macroscopic examination, Gram staining, Giemsa staining, India ink staining
Applicable age groups:
Neonates, infants, children, adults, immunocompromised
Pathogens covered:
Group B Streptococci, E. coli, Listeria monocytogenes, Haemophilus influenzae type B, Neisseria meningitidis, Streptococcus pneumoniae, Cryptococcus neoformans, Mycobacterium tuberculosis, Streptococcus suis
Safety precautions:
Class II biosafety cabinet use, aseptic handling
Reference tables/appendices:
Appendix 1 (normal CSF cell counts), Table 1 (organism identification)
Laboratory equipment:
Fuchs-Rosenthal counting chamber, centrifuge, microscope