№ lp_2_1_12486
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Supplementary figure illustrating rRNA 2’-O-methylation analysis in leukemia stem cells and healthy hematopoietic cells, with a focus on molecular profiling and gene expression analysis in AML patients.
Year: 2021
Region / City: Not specified
Theme: Hematology, Leukemia, Molecular Biology
Document Type: Research Supplementary Figure
Author: Not specified
Target Audience: Researchers in the field of leukemia and hematopoiesis
Period of Validity: Not specified
Approval Date: Not specified
Date of Changes: Not specified
Note: Contextual Description
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Characterization of Microbial Diversity at Watering Points Along the Upper Isiukhu River in Kakamega County, Kenya Using 16S rRNA Sequencing
Year: Not specified
Region / Location: Upper reaches of River Isiukhu, Kakamega County, Kenya
Geographical Coordinates: Between 34.8372 and 0.2970 (forest) and 34.7448 and 0.2557 (downstream)
Thematic Area: Microbial ecology; freshwater ecosystems; environmental microbiology
Type of Document: Original Research Article
Study Design: Cross-sectional study with random sampling
Sampling Sites: Nine sampling points along the river
Sample Size: 54 water samples and 18 sediment samples
Methodology: Genomic DNA extraction; 16S rRNA sequencing using Illumina MiSeq; taxonomic assignment with QIIME pipeline, RDP and Green Genes databases; data visualization using Emperor
Key Findings: 640,775 quality-filtered sequences; 1,549 OTUs; 99% bacteria and 1% archaea; 40 detected phyla dominated by Proteobacteria, Bacteroidetes, Firmicutes, and Verrucomicrobia
Environmental Context: Areas influenced by agriculture, livestock watering, laundry, bathing, and sewage disposal
Study Focus: Influence of anthropogenic and environmental factors on microbial assemblages
Ecosystem Type: Tropical freshwater river system
Analytical Approach: Targeted metagenomics based on 16S rRNA gene sequencing
Supplementary Materials: Phylogenetic Analyses of Parabasalia and Trichomonads Based on 18S rRNA and ITS1-5.8S-ITS2 Loci
Year: 2015
Field: Molecular biology; microbiology; phylogenetics
Subject: Parabasalia and trichomonad phylogeny based on rRNA loci
Document type: Supplementary scientific material
Content elements: Phylogenetic trees; primer table; BLAST alignment results; sequence alignments
Methods: Maximum likelihood phylogenetic analysis; BLAST sequence comparison
Genetic markers: 18S rRNA; ITS1-5.8S rRNA-ITS2
Organisms studied: Tetratrichomonas spp.; parabasalia; trichomonads
Host organisms mentioned: Cattle (bovine hosts)
Data sources: GenBank sequence accessions; cattle gastrointestinal metatranscriptome datasets
Figures: Figure S1; Figure S2
Tables: Table S1; Table S2
Supplementary files: S1–S4 sequence alignments
Primer information: Oligonucleotide primers with forward and reverse sequences
Key references cited: Hadziavdic et al. (2014); Malik et al. (2011); Cepicka et al. (2010); Benson et al. (2015)
HEMATOPOIETIC CELLULAR THERAPY PRODUCT COLLECTION, PROCESSING, AND ADMINISTRATION DOCUMENT SUBMISSION REQUIREMENTS
Year: 2021
Region / City: Omaha, NE
Theme: Hematopoietic Cellular Therapy
Document Type: Standard Operating Procedure
Organization / Institution: FACT Accreditation
Author: FACT
Target Audience: Medical Facilities, Accreditation Inspectors
Period of Validity: N/A
Date of Approval: December 14, 2021
Date of Changes: N/A
Research Database for Hematopoietic Cell Transplantation and Cellular Therapies Adult Research Consent Form and Parent/Legal Guardian Permission Form
Year: 2023
Region / City: United States
Topic: Hematopoietic Cell Transplantation, Cellular Therapies, Research Databases
Document Type: Consent Form
Organization / Institution: CIBMTR (Center for International Blood and Marrow Transplant Research)
Author: CIBMTR
Target Audience: Patients and/or parents/legal guardians of recipients of hematopoietic cell transplants or cellular therapies
Effective Period: N/A
Approval Date: N/A
Amendment Date: N/A
Supplemental Materials and Methods for Hematopoietic Stem Cell and Leukemia Research
Year: 2010–2015
Institution: University Hospitals Case Medical Center
Field: Biomedical Research / Hematology
Document Type: Research Methods Supplement
Techniques: Immunohistochemistry, Flow Cytometry, Multi-photon Intravital Imaging, Chromatin Immunoprecipitation, Luciferase Reporter Assay, Western Blot, qRT-PCR
Model Organisms: Mice (including leukemia models)
Authors: Myers JT, Barkauskas DS, Huang AY, Wang W, Yu S, Zimmerman G, Wang Y, Yu VW, Huang D, Huang X, Shim J, Huang Y, Xin W, Qiao P, Yan M, Scadden DT, Stanley P, Lowe JB, Zhou L, Yao D
Target Cells: Hematopoietic stem cells, marrow stromal cells, leukemia cells
Reagents and Antibodies: Hes1, RBPJ, JAG1, ICN1, ICN2, GFP, Annexin V, CD41, CXCL12, GAPDH
Culture Conditions: α-MEM medium, serum-free medium, supplements including mSCF and TPO
Imaging Equipment: SP5/AOBS/2-photon microscope, Leica Microsystems, Imaris software
Analytical Methods: qRT-PCR, fluorescence imaging, statistical analysis
References: Myers JT et al., Stem Cells International 2013; Myers J et al., Transfusion 2010; Wang W et al., Stem Cells 2015; Yao D et al., Blood 2011
Patient-Reported Outcomes Measures on Adult And Pediatric Receptors Of Hematopoietic Cell Transplant Developing Chronic Graft-Versus-Host Disease
Year: 2026
Region / City: Barcelona, Spain
Theme: Hematopoietic stem cell transplant, chronic graft-versus-host disease, patient-reported outcomes
Document Type: Research article
Organization / Institution: Hospital de la Santa Creu i Sant Pau, Institut d’Investigacio Contra la Leucemia Josep Carreras, Moffitt Cancer Center, St. Anna Children’s Hospital
Authors: Anna Barata, Anita Lawitschka
Target Audience: Medical professionals, researchers, clinicians, healthcare providers
Period of Effect: N/A
Approval Date: N/A
Date of Changes: N/A
Topics: 2-O-methylation rRNA HEMATOPOIETIC