№ files_lp_3_process_7_073857
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Peer-reviewed scientific research article presenting molecular analysis of microbial community composition in water and sediment samples from multiple watering points along the upper River Isiukhu in Kakamega County, Kenya.
Year: Not specified
Region / Location: Upper reaches of River Isiukhu, Kakamega County, Kenya
Geographical Coordinates: Between 34.8372 and 0.2970 (forest) and 34.7448 and 0.2557 (downstream)
Thematic Area: Microbial ecology; freshwater ecosystems; environmental microbiology
Type of Document: Original Research Article
Study Design: Cross-sectional study with random sampling
Sampling Sites: Nine sampling points along the river
Sample Size: 54 water samples and 18 sediment samples
Methodology: Genomic DNA extraction; 16S rRNA sequencing using Illumina MiSeq; taxonomic assignment with QIIME pipeline, RDP and Green Genes databases; data visualization using Emperor
Key Findings: 640,775 quality-filtered sequences; 1,549 OTUs; 99% bacteria and 1% archaea; 40 detected phyla dominated by Proteobacteria, Bacteroidetes, Firmicutes, and Verrucomicrobia
Environmental Context: Areas influenced by agriculture, livestock watering, laundry, bathing, and sewage disposal
Study Focus: Influence of anthropogenic and environmental factors on microbial assemblages
Ecosystem Type: Tropical freshwater river system
Analytical Approach: Targeted metagenomics based on 16S rRNA gene sequencing
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