№ files_lp_4_process_3_096337
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Practical laboratory worksheet detailing a student experiment measuring how different pH levels influence trypsin activity on milk protein using colorimetric analysis.
Year:
Not specified
Region / City:
Not specified
Subject:
Biology / Biochemistry
Document Type:
Laboratory Worksheet
Institution:
Not specified
Author:
Not specified
Target Audience:
Students studying enzyme activity
Experiment Date:
Not specified
Variables:
Independent – pH; Dependent – absorbance of milk solution
Method:
Colorimetric measurement of casein digestion
Enzyme:
Trypsin
Substrate:
Milk protein (casein)
Price: 8 / 10 USD
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The product description is provided for reference. Actual content and formatting may differ slightly.
Year:
2017
Subject Area:
Biosensing and Enzyme Detection
Keywords:
gelatin film, quartz crystal microbalance, trypsin, amylase, lipase, enzymatic degradation
Type of Document:
Supplementary Materials
Experimental Method:
Quartz Crystal Microbalance (QCM)
Equipment:
Maxtek RQCM (Inficon)
Temperature Conditions:
Approximately 37°C
Flow Rate:
20 μL/min
Test Media:
Air, PBS, PBS with enzymes
Enzyme Concentrations:
0.5 mg/mL and 1 mg/mL
License:
Creative Commons Attribution (CC BY) 4.0
Figures Included:
Figure 1 (a–c)
Measured Parameters:
Gelatin degradation rate, mass change, signal saturation
Field of Application:
Pancreatic enzyme sensing
Subject:
Biology
Topic:
Enzyme activity and enzyme kinetics
Focus enzyme:
Trypsin
Substrate:
Casein (milk protein)
Independent variables:
Temperature; pH; Substrate concentration; Inhibitor type (green tea, black tea)
Dependent variable:
Time taken for suspension to clear; Rate of reaction (1/t)
Control treatments:
Milk without trypsin; Trypsin with water (no inhibitor)
Materials:
3% milk suspension; 1% trypsin solution; buffer solutions (pH 2, 4, 7, 10); green tea; black tea; water baths; ice bath; colorimeter (alternative method)
Experimental methods:
Measurement of clearing time in milk suspension; Optional colorimetric measurement of light transmission
Educational level:
Laboratory session for learners
Session number:
5
Reference cited:
Qie, X. et al. (2021), Food Research International, vol. 140
Year:
2016
Region / City:
N/A
Topic:
Laboratory Procedure
Document Type:
Standard Operating Procedure (SOP)
Organization / Institution:
PNNL Lab
Author:
PNNL Lab
Target Audience:
Laboratory personnel
Duration:
N/A
Approval Date:
06/30/2016
Modification Date:
N/A
Year:
2013-2016
Region / City:
Mt. Abu, India
Topic:
Atmospheric Sciences, Tidal Activity, Nightglow Emissions
Document Type:
Data Set Description
Author:
Sovan Saha, Duggirala Pallamraju, Sunil Kumar, Fazlul I. Laskar, Nicholas M. Pedatella
Target Audience:
Researchers in Atmospheric and Space Sciences
Period of Validity:
2013-2016
Date of Approval:
N/A
Date of Changes:
N/A
Description:
Data set describing measurements of post-sunset OI 630.0 nm emissions and related atmospheric parameters for specific nights between 2013 and 2016.
Year:
2024
Region / City:
Indiana
Topic:
Historic Preservation, Section 106 Process
Document Type:
Template
Agency:
Federal Highway Administration, Indiana Department of Transportation
Author:
Federal Highway Administration
Target Audience:
Consultants, State Historic Preservation Officer, Federal and State Agencies
Validity Period:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Year:
2025
Region / City:
West Virginia
Subject:
Fire Protection Services
Document Type:
Legislation
Organization / Institution:
West Virginia Legislature
Author:
Delegate Burkhammer
Target Audience:
Government authorities, municipalities, and businesses affected by fire protection fees
Effective Date:
July 9, 2025
Date of Approval:
April 10, 2025
Date of Amendments:
Not specified
Year:
2025
Region / city:
West Virginia
Topic:
Employment Law
Document Type:
Legislative Bill
Author:
Delegates Hanshaw and Hornbuckle
Target Audience:
State Employees and Government Officials
Effective Date:
July 1, 2025
Date of Approval:
April 8, 2025
Date of Amendments:
None
Note:
Year
Year:
2020
Region / city:
Not specified
Topic:
Mortality, Preterm birth, Systematic review, Meta-analysis
Document type:
Systematic review
Organization / institution:
Not specified
Author:
Not specified
Target audience:
Researchers, Healthcare professionals
Period of validity:
Not specified
Approval date:
Not specified
Date of modifications:
Not specified
Year:
2023
Region / City:
Kansas City
Topic:
Data analysis, sports performance, celebrity influence
Document Type:
Lesson plan
Institution:
DataClassroom
Author:
DataClassroom
Target Audience:
High school students (Grade 9-10)
Duration:
1 hour 45 minutes
Approval Date:
Not specified
Date of Changes:
Not specified
Note:
Contextual description
Year:
2024
Region / City:
Samarkand
Topic:
Migratory species conservation, CMS decisions
Document Type:
Conference decisions
Organization:
CMS
Author:
Conference of the Parties to CMS
Target Audience:
Parties to the CMS
Period of Validity:
2024-2032
Approval Date:
Not specified
Amendment Date:
Not specified
Year:
2025
Region / city:
Canada
Topic:
Diet, physical activity, older adults
Document type:
Research supplementary material
Organization / institution:
NuAge Study
Author:
Didier Brassard, Nancy Presse, Stéphanie Chevalier
Target audience:
Researchers, health professionals, policymakers
Period of validity:
Not specified
Approval date:
Not specified
Date of changes:
Not specified
Year:
2025
Region / City:
West Virginia
Topic:
Unemployment Compensation
Document Type:
Legislative Act
Agency / Institution:
West Virginia Legislature
Author:
Delegate Street
Target Audience:
General Public, Employers, Employees
Period of Validity:
From July 2, 2025
Approval Date:
April 3, 2025
Amendment Date:
None
Year:
2024
Region / city:
Global
Topic:
Ethics, Regulations
Document Type:
Code of Ethics
Organization / Institution:
World Sailing
Author:
World Sailing
Target Audience:
Participants in sailing competitions, World Sailing officials, and affiliated bodies
Validity Period:
From 10 November 2024
Approval Date:
Not provided
Date of Changes:
Not provided
Year:
2021
Region / City:
N/A
Theme:
Open Science, Research Trustworthiness
Document Type:
Peer Review Decision
Organ / Institution:
Collabra
Author:
Clare Conry-Murray, Ann Mcconnon, Morgan Bower
Target Audience:
Researchers, Academics, Practitioners
Period of validity:
N/A
Approval Date:
N/A
Date of changes:
N/A
Year:
2022
Region / city:
Baghdad, Iraq
Topic:
Spinal Cord Injury, Dietary Regimen, Educational Intervention
Document type:
Research Study
Organization / institution:
University of Baghdad, College of Nursing
Authors:
Ali Abdulridha Shalash, Ayad Majid Mousa
Target audience:
Healthcare professionals, researchers, educators
Period of validity:
N/A
Approval date:
N/A
Date of changes:
N/A
Note:
Year
Topic:
Medical treatment, Workers’ compensation
Document type:
Notice
Target Audience:
Employees receiving medical treatment through MPN
Context:
Notice for employees informing them of the end of coverage under the current MPN and the transition to a new MPN, with no impact on medical treatment.
Year:
2020
Region / city:
United Kingdom
Topic:
Undergraduate medical education, COVID-19, e-learning
Document type:
Report
Organization / institution:
University of Birmingham, Clinical Skills Department
Author:
Melissa Jayne Whiles
Target audience:
Medical students, clinical teaching fellows, education and training departments
Period of validity:
2020
Date of approval:
July 4th, 2020
Date of changes:
N/A
Year:
2026
Term:
Fall 2026
Application deadline:
January 22, 2026
Institution:
North Idaho College
Program:
Practical Nursing Program (LPN)
Location:
Coeur d’Alene, Idaho
Type of document:
Application information packet
Target audience:
Prospective applicants to the Practical Nursing Program
Mandatory orientation date:
June 3, 2026
Information session date:
January 14, 2026
Delivery format:
Electronic application with required supporting documents
Admission requirements:
College transcripts, CNA certification, nursing entrance exam results
Administrative offices:
Cardinal Central Office; Health Professions & Nursing Admissions
Admissions contacts:
Student Success Navigators for Nursing