№ files_lp_3_process_9_26688
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Supplementary experimental record presenting quartz crystal microbalance measurements of gelatin film degradation under exposure to trypsin, amylase, lipase, and their mixtures, with quantified mass loss rates and signal saturation behavior.
Year: 2017
Subject Area: Biosensing and Enzyme Detection
Keywords: gelatin film, quartz crystal microbalance, trypsin, amylase, lipase, enzymatic degradation
Type of Document: Supplementary Materials
Experimental Method: Quartz Crystal Microbalance (QCM)
Equipment: Maxtek RQCM (Inficon)
Temperature Conditions: Approximately 37°C
Flow Rate: 20 μL/min
Test Media: Air, PBS, PBS with enzymes
Enzyme Concentrations: 0.5 mg/mL and 1 mg/mL
License: Creative Commons Attribution (CC BY) 4.0
Figures Included: Figure 1 (a–c)
Measured Parameters: Gelatin degradation rate, mass change, signal saturation
Field of Application: Pancreatic enzyme sensing
Price: 8 / 10 USD
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Session 5: Trypsin Activity – Investigation of Temperature, pH, Substrate Concentration and Tea Inhibitors
Subject: Biology
Topic: Enzyme activity and enzyme kinetics
Focus enzyme: Trypsin
Substrate: Casein (milk protein)
Independent variables: Temperature; pH; Substrate concentration; Inhibitor type (green tea, black tea)
Dependent variable: Time taken for suspension to clear; Rate of reaction (1/t)
Control treatments: Milk without trypsin; Trypsin with water (no inhibitor)
Materials: 3% milk suspension; 1% trypsin solution; buffer solutions (pH 2, 4, 7, 10); green tea; black tea; water baths; ice bath; colorimeter (alternative method)
Experimental methods: Measurement of clearing time in milk suspension; Optional colorimetric measurement of light transmission
Educational level: Laboratory session for learners
Session number: 5
Reference cited: Qie, X. et al. (2021), Food Research International, vol. 140
Trypsin digestion of tissue sample
Year: 2016
Region / City: N/A
Topic: Laboratory Procedure
Document Type: Standard Operating Procedure (SOP)
Organization / Institution: PNNL Lab
Author: PNNL Lab
Target Audience: Laboratory personnel
Duration: N/A
Approval Date: 06/30/2016
Modification Date: N/A
Effect of pH on Trypsin Activity: Practical Investigation
Year: Not specified
Region / City: Not specified
Subject: Biology / Biochemistry
Document Type: Laboratory Worksheet
Institution: Not specified
Author: Not specified
Target Audience: Students studying enzyme activity
Experiment Date: Not specified
Variables: Independent – pH; Dependent – absorbance of milk solution
Method: Colorimetric measurement of casein digestion
Enzyme: Trypsin
Substrate: Milk protein (casein)
Topics: Gelatin-Enabled Microsensor Trypsin