№ files_lp_3_process_7_090603
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The document describes a study on the detection and identification of Escherichia coli in poultry meat and related environmental sources, conducted in Junagadh, Gujarat, highlighting public health concerns regarding foodborne diseases and antibiotic resistance.
Year:
2024
Region / City:
Junagadh, Gujarat
Topic:
Food-borne Diseases, E. coli, Poultry Meat, Antibiotic Resistance
Document Type:
Research Study
Author:
Jangid, Malabadi et al., Geurtsen et al., Rawat, Ali & Chidrawar, Xia et al., Hamed et al., Ibrahim et al., Mohamed and Desmulliez, Gupta et al., Rahman et al.
Target Audience:
Public Health Researchers, Food Safety Experts, Academics
Period of Action:
2024
Date of Approval:
2024
Date of Modifications:
None
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The product description is provided for reference. Actual content and formatting may differ slightly.
Year:
2018
Region / City:
Gorgan, Iran
Subject:
Taxonomy of viruses
Document type:
Taxonomic proposal
Organization / Institution:
ICTV
Authors:
Mahsa Yazdi, Majid Bouzari, Ezzat Allah Ghaemi, Evelien Adriaenssens, Andrew Kropinski
Target audience:
ICTV Subcommittees
Date of approval:
June 2018
Date of revision:
N/A
Contextual description:
Taxonomic proposal for the creation of a new species, Escherichia virus Golestan, within the Kagunavirus genus, based on genomic analysis of related phages.
Year:
2023
Region / city:
Bristol, UK
Field:
Immunology, Autoimmune Diseases
Document Type:
Research article
Institution:
University of Bristol
Author:
Not specified
Target Audience:
Researchers in immunology, medical professionals
Period of validity:
Not specified
Approval Date:
Not specified
Date of amendments:
Not specified
Year:
2023
Region / city:
Singapore
Topic:
Protein structure, drug discovery
Document type:
Research article
Organization / institution:
Experimental Therapeutics Centre, Agency for Science, Technology and Research, Singapore
Author:
Yan Li, Ying Lei Wong, Michelle Yueqi Lee, Hui Qi Ng, CongBao Kang
Target audience:
Researchers, scientists in biochemistry and pharmacology
Period of validity:
N/A
Date of approval:
N/A
Date of changes:
N/A
Year:
2023
Region / City:
Seoul, Republic of Korea
Topic:
Biotechnology, Enzyme Engineering
Document Type:
Research Supplementary Information
Institution:
Korea University, Sogang University
Authors:
Yeonhwa Yu, Yongfan Shi, Young Wan Kwon, Yoobin Choi, Yusik Kim, Jeong-Geol Na, June Huh, Jeewon Lee
Target Audience:
Researchers in Chemical and Biological Engineering, Biotechnology
Period of Validity:
Not specified
Approval Date:
Not specified
Modification Date:
Not specified
Note:
Contextual Description
Year:
2023–2024
Region:
Junagadh district, Gujarat state, India
Subject:
Microbiological analysis of foodborne pathogens
Document Type:
Research article
Study Population:
Poultry meat samples and associated environmental samples
Sample Size:
350 samples
Poultry Meat Samples:
250
Environmental Samples:
100 (butcher’s hand swabs and water samples)
Target Organism:
Escherichia coli
Methods:
Culture isolation on MacConkey agar and EMB agar, biochemical tests (TSI, LIA, IMViC), PCR targeting alr gene
Incubation Temperature:
37 °C
Positive Samples:
203 (58.00%)
Positive Poultry Meat Samples:
162 (62.00%)
Positive Hand Swabs:
19 (38.00%)
Positive Water Samples:
22 (44.00%)
Laboratory Techniques:
Gram staining, catalase test, oxidase test, IMViC test, PCR amplification
Associated Institutions Referenced:
World Health Organization (WHO), Centers for Disease Control and Prevention (CDC)
Year:
2023–2024
Region:
Jammu region
Country:
India
Subject:
Virulence genes and antimicrobial resistance in Escherichia coli from neonatal ruminants
Document Type:
Scientific research article
Bacterial Species:
Escherichia coli
Host Species:
Lambs, Kids, Buffalo calves
Sample Size:
150 fecal samples
Confirmed Isolates:
123 (82%)
Virulence Genes Investigated:
stx1, stx2, eaeA, hlyA
Laboratory Methods:
Culture isolation, biochemical identification, PCR, multiplex PCR, antimicrobial susceptibility testing
Antimicrobial Testing Standard:
CLSI 2024 guidelines
Antibiotics Tested:
Enrofloxacin, Gentamicin, Imipenem, Cefoxitin, Tetracycline, Chloramphenicol, Aztreonam, Streptomycin, Ampicillin, Amoxyclav, Amikacin, Cefotaxime, Nalidixic Acid, Cefpodoxime, Erythromycin
Multidrug Resistance Prevalence:
71.43% among virulent isolates
Institutions Involved:
Government farms, SKUAST-Jammu institutional facilities, Veterinary Clinical Complex, Private holdings
Year:
2026
Region / State:
Massachusetts, USA
Topic:
Drinking Water Safety / Public Notification
Document Type:
Regulatory Guidance / Template
Agency:
Massachusetts Department of Environmental Protection (MassDEP)
Target Audience:
Public Water Systems (PWS) Operators and Local Health Authorities
Regulation Reference:
310 CMR 22.16, 22.19, 22.04
Contaminant Focus:
E. coli / Total Coliform
Delivery Methods:
Radio, Television, Hand/Direct Delivery, Posting, Newspaper Publication
Population Served:
Communities served by specific PWS, including neighboring areas if applicable
Required Actions:
Public notice issuance within 24 hours of Tier 1 violation, corrective actions, coordination with local health authorities
Note:
Year
Year:
2023
Region / city:
Bristol, UK
Field:
Immunology, Autoimmune Diseases
Document Type:
Research article
Institution:
University of Bristol
Author:
Not specified
Target Audience:
Researchers in immunology, medical professionals
Period of validity:
Not specified
Approval Date:
Not specified
Date of amendments:
Not specified
Year:
2009
Region / City:
Ithaca, New York
Field:
Biological Engineering, Microbiology
Document Type:
Research Thesis
Institution:
Cornell University
Author:
Matthew S. Russell
Target Audience:
Researchers in Biological Engineering, Microbiology
Period of Validity:
N/A
Approval Date:
N/A
Date of Changes:
N/A
Year:
2023
Region / city:
Singapore
Topic:
Protein structure, drug discovery
Document type:
Research article
Organization / institution:
Experimental Therapeutics Centre, Agency for Science, Technology and Research, Singapore
Author:
Yan Li, Ying Lei Wong, Michelle Yueqi Lee, Hui Qi Ng, CongBao Kang
Target audience:
Researchers, scientists in biochemistry and pharmacology
Period of validity:
N/A
Date of approval:
N/A
Date of changes:
N/A
Year:
2023
Region / City:
Seoul, Republic of Korea
Topic:
Biotechnology, Enzyme Engineering
Document Type:
Research Supplementary Information
Institution:
Korea University, Sogang University
Authors:
Yeonhwa Yu, Yongfan Shi, Young Wan Kwon, Yoobin Choi, Yusik Kim, Jeong-Geol Na, June Huh, Jeewon Lee
Target Audience:
Researchers in Chemical and Biological Engineering, Biotechnology
Period of Validity:
Not specified
Approval Date:
Not specified
Modification Date:
Not specified
Note:
Contextual Description
Type of document:
Public health notice template
Subject:
E. coli contamination in drinking water
Contaminant:
Escherichia coli (E. coli)
Regulatory classification:
Tier 1 public notice
Legal basis:
OAR 333-061-0042(3); OAR 333-061-0042(4); OAR 333-061-0040(1)
Issuing authority:
Oregon Health Authority Drinking Water Services
Geographical scope:
Oregon
Target audience:
Consumers served by the affected water system
Distribution methods:
Radio; Television; Hand delivery; Posting in conspicuous locations; Newspaper
Required action:
Boil water before consumption
Health risk groups identified:
Infants; elderly; individuals with severely compromised immune systems
Contact information referenced:
EPA Safe Drinking Water Hotline (800) 426-4791; Oregon Health Authority (971) 673-0405
Document code:
OHA-DWS 2/2023
Publication date:
2/2023
Year:
2022
Region / City:
[Water System Name]
Topic:
Water Quality
Document Type:
Template
Agency / Institution:
[Water System Name]
Author:
[Water System Name]
Target Audience:
Consumers of the water system
Period of Validity:
Ongoing
Approval Date:
[Enter date]
Date of Modifications:
[Enter date if applicable]
Year:
Not specified
Region / City:
Not specified
Topic:
Biotechnology, Genetic Engineering
Document Type:
Protocol
Institution:
Not specified
Author:
Not specified
Target Audience:
Researchers in molecular biology and genetic engineering
Period of Validity:
Not specified
Approval Date:
Not specified
Modification Date:
Not specified
Trial Title:
Eculizumab in Shiga-Toxin Producing E. Coli Haemolytic Uraemic Syndrome (ECUSTEC): A Randomised, Double-Blind, Placebo-Controlled Trial
Acronym:
ECUSTEC
Type of Document:
Patient Information Sheet
Study Design:
Randomised, Double-Blind, Placebo-Controlled Trial
Medical Condition:
Shiga-Toxin Producing E. Coli Haemolytic Uraemic Syndrome (STEC HUS)
Investigational Medicinal Product:
Eculizumab
Comparator:
Placebo
Target Population:
Children and Young People Aged 16–18 Years Diagnosed with STEC HUS
Sample Size:
134 Participants
Geographical Scope:
United Kingdom
Study Duration:
Four Years Recruitment with 12 Months Follow-Up per Participant
Co-ordinating Centre:
Birmingham Clinical Trials Unit (BCTU)
Intervention Schedule:
Two Doses Within One Week, First Dose Within 48 Hours of Admission
Primary Objective:
To Assess Whether Eculizumab Reduces Disease Severity in STEC HUS
Consent Requirement:
Written Informed Consent Required
Voluntary Participation:
Yes
Year:
[unspecified]
Region / County:
[unspecified]
Type of Document:
Public health advisory
Issuing Organization:
[unspecified] Water System
Target Audience:
Residents and users of the water supply
Date of Detection:
[unspecified]
Recommended Action:
Boil water before use
Expected Resolution Date:
[unspecified]
Contact Information:
[unspecified]
Year:
2023–2024
Region:
Junagadh district, Gujarat state, India
Subject:
Microbiological analysis of foodborne pathogens
Document Type:
Research article
Study Population:
Poultry meat samples and associated environmental samples
Sample Size:
350 samples
Poultry Meat Samples:
250
Environmental Samples:
100 (butcher’s hand swabs and water samples)
Target Organism:
Escherichia coli
Methods:
Culture isolation on MacConkey agar and EMB agar, biochemical tests (TSI, LIA, IMViC), PCR targeting alr gene
Incubation Temperature:
37 °C
Positive Samples:
203 (58.00%)
Positive Poultry Meat Samples:
162 (62.00%)
Positive Hand Swabs:
19 (38.00%)
Positive Water Samples:
22 (44.00%)
Laboratory Techniques:
Gram staining, catalase test, oxidase test, IMViC test, PCR amplification
Associated Institutions Referenced:
World Health Organization (WHO), Centers for Disease Control and Prevention (CDC)
Year:
2023–2024
Region:
Jammu region
Country:
India
Subject:
Virulence genes and antimicrobial resistance in Escherichia coli from neonatal ruminants
Document Type:
Scientific research article
Bacterial Species:
Escherichia coli
Host Species:
Lambs, Kids, Buffalo calves
Sample Size:
150 fecal samples
Confirmed Isolates:
123 (82%)
Virulence Genes Investigated:
stx1, stx2, eaeA, hlyA
Laboratory Methods:
Culture isolation, biochemical identification, PCR, multiplex PCR, antimicrobial susceptibility testing
Antimicrobial Testing Standard:
CLSI 2024 guidelines
Antibiotics Tested:
Enrofloxacin, Gentamicin, Imipenem, Cefoxitin, Tetracycline, Chloramphenicol, Aztreonam, Streptomycin, Ampicillin, Amoxyclav, Amikacin, Cefotaxime, Nalidixic Acid, Cefpodoxime, Erythromycin
Multidrug Resistance Prevalence:
71.43% among virulent isolates
Institutions Involved:
Government farms, SKUAST-Jammu institutional facilities, Veterinary Clinical Complex, Private holdings
Note:
Year
Theme:
Gene cloning, plasmid manipulation, PCR, bacterial transformation
Document type:
Laboratory protocol
Target audience:
Researchers, laboratory staff
Context:
Detailed laboratory protocol for cloning a gene of interest into a luciferase plasmid in E. coli, covering all steps from PCR amplification to bacterial transformation and screening.