№ files_lp_4_process_1_50310
File format: docx
Character count: 7219
File size: 24 KB
Note:
Year
Theme:
Gene cloning, plasmid manipulation, PCR, bacterial transformation
Document type:
Laboratory protocol
Target audience:
Researchers, laboratory staff
Context:
Detailed laboratory protocol for cloning a gene of interest into a luciferase plasmid in E. coli, covering all steps from PCR amplification to bacterial transformation and screening.
Price: 8 / 10 USD
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The product description is provided for reference. Actual content and formatting may differ slightly.
Type:
DNA sequence
Molecule:
plasmid
Sequence features:
T7 promoter, cloning sites, regulatory regions
Content focus:
recombinant DNA construct
Format:
nucleotide sequence
Intended use:
molecular biology reference
Source type:
primary sequence data
Year:
2026
Region / city:
Not specified
Topic:
Genomic manipulation, yeast transformation, molecular biology
Document type:
Protocol
Organization / Institution:
Not specified
Author:
Not specified
Target audience:
Researchers, Molecular biologists
Period of validity:
Not specified
Approval date:
Not specified
Date of amendments:
Not specified
Note:
Contextual description
Year:
1994
Region / City:
Seattle, WA
Topic:
Plasmid Shipment
Document Type:
Research Methodology
Institution:
Fred Hutchinson Cancer Center
Authors:
Guy J Rosman, A. Dusty Miller
Target Audience:
Researchers in molecular biology
Date of Approval:
1994
Date of Last Modification:
Not specified
Storage Duration:
2 months at 40°C
Method:
Shipping plasmids in sealed paper for safe delivery
Contextual Description:
Research methodology for shipping plasmids using a new technique that prevents cross-contamination and damage during transit.
Date:
22JAN2021
Version:
3.0
Protocol Number:
TN-27
Study Name:
TOPPLE T1D Study – Tolerance Using Plasmid T1D
Phase:
Phase 1
Study Design:
Multiple ascending dose trial
Investigational Product:
NNC0361-0041
Condition:
Type 1 diabetes mellitus
Sponsor Collaboration:
Novo Nordisk
Research Network:
Type 1 Diabetes TrialNet
Supporting Institutions:
National Institutes of Health; National Institute of Diabetes and Digestive and Kidney Diseases
Study Site:
Stanford University
Research Physician:
Darrell Wilson, MD
Target Population:
Adults diagnosed with type 1 diabetes within the previous 48 months
Planned Enrollment:
Approximately 48 participants
Geographic Scope:
United States, Canada, Europe, Australia
Therapeutic Approach:
Plasmid-based immunotherapy administered subcutaneously
Year:
2024
Region / City:
United States
Theme:
Public Assistance / Fraud Prevention
Document Type:
Operations Memorandum
Organization / Institution:
Department of Human Services
Author:
Robert Hixson
Target Audience:
Executive Directors, County Assistance Offices
Period of Validity:
October 1, 2022 - September 30, 2024
Approval Date:
April 12, 2024
Date of Changes:
August 2, 2023
Context:
This operations memorandum outlines procedures for replacing SNAP benefits stolen via electronic fraud, including card skimming, cloning, and phishing scams, for households affected between October 1, 2022, and September 30, 2024.
Type:
DNA sequence
Molecule:
plasmid
Sequence features:
T7 promoter, cloning sites, regulatory regions
Content focus:
recombinant DNA construct
Format:
nucleotide sequence
Intended use:
molecular biology reference
Source type:
primary sequence data
Authors:
Vivianne J Goosens; Kenneth T Walker; Silvia M Aragon; Amritpal Singh; Vivek R Senthivel; Linda Dekker; Joaquin Caro-Astorga; Marianne L A Buat; Wenzhe Song; Koon-Yang Lee; Tom Ellis
Corresponding author:
Tom Ellis
Affiliation:
Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK
Note:
Affiliation
Department of Bioengineering, Imperial College London, London SW7 2AZ, UK
Institution:
Imperial College London
Field:
Synthetic biology; Engineered Living Materials; bacterial cellulose; modular cloning
Organisms:
Komagataeibacter rhaeticus; Komagataeibacter xylinus; Komagataeibacter hansenii; Escherichia coli
Methodology:
Golden Gate DNA assembly; Type IIS restriction enzymes; modular plasmid-based cloning
System described:
Komagataeibacter Tool Kit (KTK)
Application:
Multigene construct assembly and programmed protein secretion in cellulose-producing bacteria
Document type:
Scientific research article
Research focus:
Development and demonstration of a hierarchical modular cloning toolkit for cellulose-producing bacteria
Year:
2002
Region / city:
Canada
Theme:
Molecular biology
Document type:
Scientific research data
Organization / institution:
Jones et al.
Author:
Jones et al.
Target audience:
Researchers in molecular biology
Period of validity:
Not specified
Approval date:
Not specified
Date of changes:
Not specified
Year:
Not specified
Region / City:
Not specified
Theme:
Genetics, Cloning, Selective Breeding
Document Type:
Textbook Chapter
Organization / Institution:
Not specified
Author:
Not specified
Target Audience:
Students of Biology / Genetics
Period of Validity:
Not specified
Approval Date:
Not specified
Date of Amendments:
Not specified
Year:
2026
Institution:
University/College (unspecified)
Course:
BIO 510
Type of document:
Exam
Format:
Open book and closed book sections
Topics:
PCR, restriction enzymes, plasmid preparation, DNA ligation, ionizing radiation, enzymatic reactions, bacterial transformation
Target audience:
Undergraduate or graduate students in molecular biology
Source references:
NEB catalog, lecture topics, lab exercises, assigned readings
Date administered:
2026
Year:
N/A
Region / city:
N/A
Theme:
Gene cloning, CRISPR, Primer design
Document type:
Supplementary file
Organization / institution:
N/A
Author:
N/A
Target audience:
Researchers in molecular biology
Validity period:
N/A
Approval date:
N/A
Modification date:
N/A
Context:
A supplementary document listing sequences of primers for gene cloning and construction of various reporter vectors related to CRISPR-based studies.
Year:
2026
Region / City:
Mustansiriyah University, College of Science
Subject:
Genetic Engineering
Document Type:
Educational Material
Institution:
Mustansiriyah University
Author:
Dr. Rasha Mohammed Al-Oqaili
Target Audience:
Biology students and researchers
Period of Action:
N/A
Approval Date:
N/A
Date of Changes:
N/A
Reporting Period:
Final Report
Proposal:
2259 R-gene Clusters for Phytophthora Sojae Resistance: Cloning Rps Genes
Project Number:
2259
Committee:
Production
Target Area:
Supply
Project Start Date:
1/1/2012
Project End Date:
12/31/2012
Project Status:
Completed
Institution:
The Ohio State University
Lead Researcher:
Anne Dorrance
Collaborating Researchers:
M. A. Graham; S. A. Whitham; J. Carroll; L. M. Lincoln
Research Field:
Plant Pathology; Soybean Genetics
Study Organism:
Soybean (Glycine max)
Pathogen Studied:
Phytophthora sojae
Key Genes Investigated:
Rps2; Rps3a; Rps3c; Rps8
Genomic Regions Studied:
Soybean chromosomes 13 and 18
Methods:
BAC sequencing; genetic linkage analysis; SSR and PAMSA markers; virus-induced gene silencing; vascular puncture inoculation
Mapping Populations:
L83-570 × PI399073 F3:4; L92-7857 × PI399073 F3:4; Williams × PI 399073 BC4F5:6
Number of Pathogen Isolates Tested:
75
Associated Academic Work:
M.S. thesis by A. Guadi (2012)
Publication and Presentation Year:
2012
Document Type:
Project Status Report
Version:
2.0
Creation Date:
2/12/2013
Last Modified:
2/12/2013
Note:
Year
Topic:
Cloning, Genetics, Science Education
Document Type:
Educational Lesson
Organization / Institution:
Learn.Genetics
Target Audience:
Students, Educators, Biology Enthusiasts
Year:
2008
Region / city:
N/A
Topic:
Molecular Biology, Genetic Engineering
Document Type:
Scientific Article
Institution / Organization:
N/A
Author:
Weber K, Bartsch U, Stocking C, et al.
Target Audience:
Researchers, Molecular Biologists
Validity Period:
N/A
Approval Date:
2008/03/26
Modification Date:
N/A
Keywords:
Lentiviral vector, Gene cloning, AXL overexpression, Co-immunoprecipitation, Western blot, MPN
Context:
Scientific article detailing the methods of cloning LeGO vectors and producing lentiviral particles for genetic research and analysis.