№ lp_1_2_45757
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The document presents a research article detailing the enzymatic synthesis of a key building block for Lusutrombopag.
Year: 2019
Region / City: Tianjin, China
Subject: Enzymatic Synthesis, Biocatalysis
Document Type: Research Article
Organization / Institution: National Technology Innovation Center of Synthetic Biology, Tianjin Institute of Industrial Biotechnology, Tianjin University of Science & Technology
Authors: Yunfeng Cui, Yangyang Ji, Xi Chen, Jianjiong Li, Jinhui Feng, Qing Zhao, Peiyuan Yao, Qiaqing Wu, Dunming Zhu
Target Audience: Researchers in the field of synthetic biology, biocatalysis, and enzymology
Period of Action: N/A
Approval Date: N/A
Date of Amendments: N/A
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Supporting document 1 - Risk and technical assessment – Application A1183 Enzymatic production of rebaudioside E from stevia leaf extract
Year: 2020
Region / city: Australia / New Zealand
Topic: Food Standards Assessment
Document Type: Technical Assessment Report
Agency: Food Standards Australia New Zealand (FSANZ)
Author: FSANZ
Target Audience: Regulatory Authorities, Food Safety Experts
Period of validity: Ongoing
Approval Date: 14 May 2020
Date of changes: None
Contextual Description: This document provides a technical and risk assessment report regarding the approval process for the enzymatic production of rebaudioside E, a steviol glycoside, derived from stevia leaf extract for use as a food additive in Australia and New Zealand.
Be careful what you eat: an analysis of enzymatic functional and structural effects due to -amanitin toxicity
Year: 2018
Region / city: Alfred, NY
Subject: Biology, Biochemistry
Document type: Research article
Organization / institution: Alfred University, Department of Biology
Author: Geoffrey Lippa
Target audience: Students in biological sciences
Period of validity: N/A
Date of approval: N/A
Date of changes: N/A
Extended Methods for Characterization of AB598, a CD39 Enzymatic Inhibitory Antibody for the Treatment of Solid Tumors
Year: Not specified
Country: United States
Institution: Arcus Biosciences, Inc.
Location: Hayward, California
Document Type: Scientific methodological supplement
Research Field: Cancer immunotherapy; molecular and cellular biology
Subject: Experimental characterization of the anti-CD39 antibody AB598
Author: Amy E. Anderson; Kaustubh Parashar; Ke Jin; Julie Clor; Carlo E. Stagnaro; Urvi Vani; Jaskirat Singh; Ada Chen; Yihong Guan; Priyanka Talukdar; Pavithra Sathishkumar; Damie J. Juat; Hema Singh; Ritu Kushwaha; Xiaoning Zhao; Angelo Kaplan; Lisa Seitz; Matthew J. Walters; Ester Fernandez-Salas; Nigel P. C. Walker; Christine E. Bowman
Corresponding Author: Christine E. Bowman
Organization Address: Arcus Biosciences Inc., 3928 Point Eden Way, Hayward, CA 94545
Contact Email: [email protected]
Contact Phone: 510-694-6200
Biological Target: CD39 (ENTPD1)
Experimental Techniques: Surface plasmon resonance (SPR); flow cytometry; luminescence assays; CRISPR knockout; qPCR; western blot
Biological Materials: Human monocytes; PBMCs; B cells; SK-MEL-5 cells; MOLP8 cells; whole blood from healthy donors
Study Focus: Binding kinetics, enzymatic inhibition, ATP hydrolysis, and CD39 surface expression following exposure to AB598
New HS-GC/FID and traditional enzymatic methods for blood alcohol analysis in myocardial infarction patients in Libya
Year: 2026
Region / City: Tripoli, Libya
Subject: Blood alcohol analysis in myocardial infarction patients
Document type: Comparative research study
Institution / Organization: Judicial Expertise and Research Center / Ministry of Justice; Faculty of Pharmacy, University of Tripoli
Authors: Mohammed S. ALrheem, Doaa A. Mansour, Asma O. Jebril
Target audience: Forensic and medical professionals
Sample size: 48 individuals, including MI patients and healthy controls
Methods: Head space-gas chromatography/flame ionization detector (HS-GC/FID) and Lactate dehydrogenase (LDH) enzymatic technique
Statistical analysis: IBM SPSS Version 21, descriptive analysis, Mann-Whitney U test, Pearson correlation
Key findings: LDH method shows significant differences in BAC between healthy and MI patients, HS-GC/FID method shows no significant differences; correlation between LDH levels and BAC concentration R² = 0.76
Recommendations: Larger sample sizes for future studies and preference for more accurate techniques in forensic analysis
Topics: S-1-3-bromo-2-methoxyphenylethanol Lusutrombopag Enzymatic