№ files_lp_4_process_3_132600
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List of oligonucleotide primers used for various genetic manipulations and vector constructions, including genotyping and cloning.
Note:
Year
Topic:
Genotyping and vector construction
Document Type:
Supplementary table
Target Audience:
Researchers in molecular biology
Price: 8 / 10 USD
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The product description is provided for reference. Actual content and formatting may differ slightly.
Year:
2023
Region / City:
China
Subject:
Molecular detection of tick-borne pathogens
Document type:
Research Supplementary Table
Author:
Liu et al., 2012; Guo et al., 2019; Hazihan et al., 2019; Li et al., 2016; Cao et al., 2013; Peng et al., 2019; Reye et al., 2012
Target audience:
Researchers, Molecular biologists
Action period:
N/A
Approval date:
N/A
Amendment date:
N/A
Gene target:
ipaH, ipaB, ipaC, ipaD, ipgD, Sen (ShET-2), virA
Year:
Not specified
Region / City:
Not specified
Topic:
Molecular Biology / Genetics
Document Type:
Supplementary Information
Organization / Institution:
Not specified
Author:
Not specified
Target Audience:
Researchers in microbiology or genetics
Date of Approval:
Not specified
Date of Modifications:
Not specified
Year:
2012
Region / city:
CDC
Topic:
Molecular Epidemiology, Genotyping
Document type:
Protocol
Organization:
CDC
Author:
CDC
Target audience:
Laboratories, Research Institutions
Period of validity:
N/A
Approval date:
03/06/2012
Date of amendments:
N/A
Purpose:
Amplification of 2 fragments from the rubella virus (RuV) envelope protein 1 (E1) coding region
PCR product sizes:
Fragment 1 (480 nts), Fragment 2 (633 nts)
Required equipment:
Thermocycler, Micropipettors, Class II biological safety cabinet
RNA preparation:
Extraction from clinical samples or cell cultures
Primers:
RV8633, RV8945, RV9112, RV9577
Control RNA:
Dried rubella RNA control
Handling instructions:
Store primers and control RNA at specified temperatures, prepare working solutions before use
Safety recommendations:
Use dedicated equipment and workspaces for RNA procedures
Contextual description:
Protocol for Rubella virus genotyping using RT-PCR to amplify specific RNA fragments, primarily for molecular epidemiological research.
Re-Approval Date:
12/05/2024
Year:
2024
Region / City:
Northeastern University
Subject:
Animal Research Methods
Document Type:
Policy
Organization / Institution:
Northeastern University Institutional Animal Care and Use Committee (IACUC)
Author:
Institutional Animal Care and Use Committee (IACUC)
Target Audience:
Researchers, Animal Care Staff
Period of Validity:
Indefinite until the next review
Approval Date:
12/05/2024
Modification Date:
N/A
Year:
2023
Region / City:
Not specified
Topic:
Genotyping, ACE Gene, Primers
Document Type:
Research Supplementary Table
Institution:
Not specified
Author:
Not specified
Target Audience:
Researchers, Geneticists
Period of validity:
Not specified
Approval Date:
Not specified
Date of changes:
Not specified
Research field:
Genetic epidemiology
Topic:
Genetic association analysis of psoriasis and psoriatic arthritis
Document type:
Research methods and supplementary materials description
Dataset:
Immunochip dataset
Genotyping centres:
Wellcome Trust Sanger Institute; Centre for Public Health Genomics at the University of Virginia; Arthritis Research UK Centre for Genetics and Genomics at the University of Manchester; University of Queensland Diamantina Institute; Trinity Translational Medicine at the University of Dublin
Genotyping software:
GenomeStudio Data Analysis Software Platform (Genotyping Module v1.8.4)
Quality control tools:
PLINK v1.07; EIGENSOFT v4.2; flashpca
Statistical methods:
Identity-by-descent analysis; principal component analysis; logistic regression; Hardy–Weinberg equilibrium filtering
Genetic markers:
SNPs from Immunochip array
Genomic region of interest:
Major Histocompatibility Complex (MHC)
Genes analyzed:
HLA-C; HLA-B; HLA-A
Phenotypes studied:
Psoriasis; Psoriatic arthritis
Population reference datasets:
HapMap3 CEU; CHB; JPT; YRI
Variables analyzed:
Allele frequency; genotype call rate; heterozygosity; age of disease onset
Associated amino acid position:
Position 45 of HLA-B
Year:
2023
Region / City:
Denmark
Theme:
Cervical Cancer Screening, HPV Testing, DNA Methylation
Document Type:
Research Article
Organization / Institution:
University Research Clinic for Cancer Screening, Aarhus University
Authors:
Mette Tranberg, Severien Van Keer, Albertus T Hesselink, Pia Nørgaard, Rikke Brøndum, Chunsen WU, Line Winther Gustafson, Anne Hammer, Pinar Bor, Karen Omann Binderup, Christina Blach, Alex Vorsters, Renske Steenbergen
Target Audience:
Researchers, Healthcare Professionals, Medical Community
Period of Application:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Study Registration:
Clinicaltrials.gov: NCT05065853
Keywords:
DNA methylation, cervical cancer screening, HPV DNA testing, urinary HPV testing, early detection of cancer/methods
Abstract:
The study evaluates the performance of ASCL1/LHX8 DNA methylation markers and HPV genotyping in first-void urine samples for detecting high-grade cervical intraepithelial neoplasia (CIN2+) and cervical cancer in HPV-positive women, comparing it with clinician-collected cervical samples.
Year:
2024
Region / City:
Detroit, MI
Topic:
Occupational Health, Post-Exposure Management
Document Type:
Safety Protocol
Organization / Institution:
Wayne State University
Author:
Henry Ford’s Occupational Health Program, OEHS
Target Audience:
WSU researchers, medical professionals
Period of Validity:
Ongoing
Approval Date:
2024
Modification Date:
Not specified
Year:
2024
Region / City:
Africa
Topic:
Vector Control, Malaria, Genetically Modified Organisms
Document Type:
Consultancy Announcement
Organization:
African Union Development Agency-NEPAD (AUDA-NEPAD)
Author:
Not specified
Target Audience:
Individual Consultants
Period of Validity:
Not specified
Approval Date:
Not specified
Modification Date:
Not specified
Year:
2023
Region / City:
San Francisco
Topic:
Physics, Vector Addition
Document Type:
Educational Activity
Institution:
Northern State University
Author:
Unknown
Target Audience:
Physics Students, Teachers
Effective Period:
N/A
Approval Date:
N/A
Date of Changes:
N/A
Contextual Description:
Educational activity designed for physics students to explore vector addition and resultant displacement through real-world examples of travel directions.
Year:
Not specified
Region / City:
Not specified
Theme:
Viral vectors for Parkinson’s disease research
Document Type:
Request Form
Organization / Institution:
University of Iowa, Viral Vector Core Facility
Author:
Not specified
Target Audience:
Researchers from non-profit and for-profit entities
Period of Validity:
Not specified
Approval Date:
Not specified
Modification Date:
Not specified
Note:
Year
Subject:
Gene Transfer, Targeting, and Therapeutics
Document Type:
Request Form
Organization / Institution:
Salk Institute
Target Audience:
Researchers and Lab personnel
Context:
A form for requesting viral vectors from the Gene Transfer, Targeting, and Therapeutics Facility at the Salk Institute for scientific research purposes.
Note:
Year
Author:
Sheng Fan, John Wheeler, David J. Prior, Marianne Negrini, Andrew J. Cross, Travis F. Hager, David L. Goldsby, David Wallis
Year:
2026
Region / City:
La Jolla, CA
Topic:
Gene Transfer, Viral Vectors
Document Type:
Request Form
Organization / Institution:
Salk Institute for Biological Studies
Author:
GT3 Core Facility
Target Audience:
Researchers, Investigators
Validity Period:
N/A
Approval Date:
N/A
Modification Date:
N/A
Facility:
Gene Transfer, Targeting and Therapeutics Facility
Institution:
Salk Institute for Biological Studies
Document Type:
Viral Vector Request Form
Subject:
Stock Reporter Viral Vectors
Submission Method:
Email to [email protected]
Required Information:
Principal Investigator, Lab Contact, Order Date, Fund Number or PO Number, Billing and Shipping Address
Aliquot Size AAV:
25 µl
Aliquot Size Lentiviral, Retroviral, Rabies:
5 µl
Vector Categories:
rAAV, Cre, DIO, FLEX, DO, Lentivirus, AAV Serotype Kits
Source References:
Addgene; Deisseroth Lab (Stanford University)
Material Transfer Requirement:
MTA required for Deisseroth Lab vectors
Titer Information:
Representative lot titers provided; actual titers may vary
Year:
Not specified
Region / City:
Not specified
Subject:
Physics, Mathematics
Document Type:
Educational Worksheet
Organization / Institution:
Not specified
Author:
Not specified
Target Audience:
Students, Physics and Mathematics learners
Period of Validity:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Contextual description:
Educational worksheet on vector addition methods in algebraic, trigonometric, and component form.
Year:
2023
Region / City:
Not specified
Theme:
Image formats, graphics types
Document Type:
Educational
Organization:
Not specified
Author:
Not specified
Target Audience:
Individuals interested in digital imaging and graphics
Validity Period:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Gene:
Gene
Year:
Not specified
Region / City:
Not specified
Topic:
Primer sequences for vector construction and qRT-PCR
Document Type:
Scientific supplementary data
Institution:
Not specified
Author:
Not specified
Target Audience:
Researchers in molecular biology
Period of Effect:
Not specified
Approval Date:
Not specified
Amendment Date:
Not specified
Context:
Document provides detailed sequences for vector construction primers and qRT-PCR primers used in gene expression studies.
Year:
2017
Award Date:
09-28-2017
Country:
United States
City:
Frederick, MD
Agency:
Department of Veterans Affairs
Contracting Office:
Strategic Acquisition Center – Frederick
Solicitation Number:
VA119A-16-R-0349
Award Number:
VA119A-17-D-0081 and related contract numbers
Program Name:
Veteran Enterprise Contracting for Transformation and Operational Readiness (VECTOR)
Contract Type:
IDIQ
Minimum Contract Amount:
$2,500.00
Number of Awardees:
68
Primary Awardee:
Academy Solutions Group, LLC
NAICS Code:
541611
Point of Contact:
Joshua Dean
Contact Email:
[email protected]
Revision Date:
March 2010
Source System:
FedBizOpps
Year:
2026
Region / city:
Global
Topic:
Gene therapy, AAV vectors
Document type:
Research paper
Author:
Multiple authors
Target audience:
Researchers, medical professionals, biotechnology specialists
Period of action:
Ongoing
Date of approval:
Not specified
Date of modifications:
Not specified