№ files_lp_3_process_7_035614
File format: docx Character count: 10283 File size: 36 KB
Year: 2012
Region / city: CDC
Topic: Molecular Epidemiology, Genotyping
Document type: Protocol
Organization: CDC
Author: CDC
Target audience: Laboratories, Research Institutions
Period of validity: N/A
Approval date: 03/06/2012
Date of amendments: N/A
Purpose: Amplification of 2 fragments from the rubella virus (RuV) envelope protein 1 (E1) coding region
PCR product sizes: Fragment 1 (480 nts), Fragment 2 (633 nts)
Required equipment: Thermocycler, Micropipettors, Class II biological safety cabinet
RNA preparation: Extraction from clinical samples or cell cultures
Primers: RV8633, RV8945, RV9112, RV9577
Control RNA: Dried rubella RNA control
Handling instructions: Store primers and control RNA at specified temperatures, prepare working solutions before use
Safety recommendations: Use dedicated equipment and workspaces for RNA procedures
Contextual description: Protocol for Rubella virus genotyping using RT-PCR to amplify specific RNA fragments, primarily for molecular epidemiological research.
Price: 8 / 10 USD
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Topics: 2-fragment CDCRT-PCR Invitrogen