№ files_lp_4_process_2_59706
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News report describing the public claim by researcher He Jiankui about the birth of gene-edited twins with a modified CCR5 gene intended to confer resistance to HIV and the immediate scientific and ethical reactions surrounding the announcement during the 2018 genome editing summit in Hong Kong.
Year: 2018
Date of publication: November 26, 2018
Location: Hong Kong, China
Topic: Human genome editing and bioethics
Document type: News article
Publication: Science
Author: Dennis Normile
Individuals mentioned: He Jiankui; Anthony Fauci; Pablo Tebas; Julian Savulescu; Sarah Chan; Jennifer Doudna; Richard Hynes; Alta Charo
Organizations: Southern University of Science and Technology; Chinese Society for Cell Biology; National Institute of Allergy and Infectious Diseases; University of Pennsylvania; University of Oxford; University of Edinburgh; University of California, Berkeley; National Academies of Sciences, Engineering, and Medicine; Nuffield Council on Bioethics
Scientific technique: CRISPR-Cas9 genome editing
Biological target: CCR5 gene in human embryos
Context event: International Summit on Human Genome Editing
Subjects of research: Gene-edited twin girls referred to as Lula and Nana
Research aim stated: Introduction of CCR5 mutation associated with resistance to HIV infection
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CRISPR Gene Editing: Designing the gRNA and Donor TemplatePiwi Matters Adaptation
Year: 2023
Region / city: Princeton, NJ
Topic: Gene editing, CRISPR/Cas9
Document type: Educational project
Organization / institution: Princeton University
Author: Laurel Lorenz
Target audience: Undergraduate students, molecular biology researchers
Period of validity: N/A
Approval date: N/A
Date of changes: N/A
Construction and application of a piggyBac CRISPR library in BmE cells
Year: 2026
Organism: Bombyx mori
Cell line: BmE embryonic cells
Technique: CRISPR-Cas9 genome editing
Vector: piggyBac transposon
Library size: 94,000 sgRNAs
Selection marker: Puromycin, Zeocin
Promoters: hsp70, IE2, U6
Tags: Flag, HA, EGFP
Oligonucleotide synthesis: Beijing Genomics Institute (BGI), China
Assembly method: Gibson Assembly, overlap PCR
Transfection reagent: X-tremeGENE HP DNA Transfection Reagent
Integration efficiency: ~25% under standard conditions
Duration of selection: 2 months
Topics: Bombshell Gene-Edited CRISPR