№ files_lp_3_process_9_33672
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This document describes a project focused on CRISPR/Cas9 gene editing and the design of gRNA and donor templates for the Piwi mutation in Drosophila, aimed at teaching students the immune functions of CRISPR and how to apply it in gene editing.
Year: 2023
Region / city: Princeton, NJ
Topic: Gene editing, CRISPR/Cas9
Document type: Educational project
Organization / institution: Princeton University
Author: Laurel Lorenz
Target audience: Undergraduate students, molecular biology researchers
Period of validity: N/A
Approval date: N/A
Date of changes: N/A
Price: 8 / 10 USD
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Supplemental Table 1 gRNA Sequences used to generate CYP19A2-/- and CYP19A1-/-/ CYP19A2-/-/PTGS2-/- embryos.
Year: 2026
Region / city: Not specified
Subject: Genetics
Document type: Table
Organization / institution: Not specified
Author: Not specified
Target audience: Researchers in genetic engineering
Action period: Not specified
Approval date: Not specified
Modification date: Not specified
CRISPR Bombshell: Chinese Researcher Claims Creation of Gene-Edited Twins Resistant to HIV, November 2018
Year: 2018
Date of publication: November 26, 2018
Location: Hong Kong, China
Topic: Human genome editing and bioethics
Document type: News article
Publication: Science
Author: Dennis Normile
Individuals mentioned: He Jiankui; Anthony Fauci; Pablo Tebas; Julian Savulescu; Sarah Chan; Jennifer Doudna; Richard Hynes; Alta Charo
Organizations: Southern University of Science and Technology; Chinese Society for Cell Biology; National Institute of Allergy and Infectious Diseases; University of Pennsylvania; University of Oxford; University of Edinburgh; University of California, Berkeley; National Academies of Sciences, Engineering, and Medicine; Nuffield Council on Bioethics
Scientific technique: CRISPR-Cas9 genome editing
Biological target: CCR5 gene in human embryos
Context event: International Summit on Human Genome Editing
Subjects of research: Gene-edited twin girls referred to as Lula and Nana
Research aim stated: Introduction of CCR5 mutation associated with resistance to HIV infection
Construction and application of a piggyBac CRISPR library in BmE cells
Year: 2026
Organism: Bombyx mori
Cell line: BmE embryonic cells
Technique: CRISPR-Cas9 genome editing
Vector: piggyBac transposon
Library size: 94,000 sgRNAs
Selection marker: Puromycin, Zeocin
Promoters: hsp70, IE2, U6
Tags: Flag, HA, EGFP
Oligonucleotide synthesis: Beijing Genomics Institute (BGI), China
Assembly method: Gibson Assembly, overlap PCR
Transfection reagent: X-tremeGENE HP DNA Transfection Reagent
Integration efficiency: ~25% under standard conditions
Duration of selection: 2 months
Topics: TemplatePiwi gRNA CRISPR