№ files_lp_4_process_3_132620
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Detailed experimental protocol for generating a genome-wide CRISPR sgRNA library in B. mori BmE cells using piggyBac transposon vectors and selection with antibiotics to establish stably modified cell lines.
Year: 2026
Organism: Bombyx mori
Cell line: BmE embryonic cells
Technique: CRISPR-Cas9 genome editing
Vector: piggyBac transposon
Library size: 94,000 sgRNAs
Selection marker: Puromycin, Zeocin
Promoters: hsp70, IE2, U6
Tags: Flag, HA, EGFP
Oligonucleotide synthesis: Beijing Genomics Institute (BGI), China
Assembly method: Gibson Assembly, overlap PCR
Transfection reagent: X-tremeGENE HP DNA Transfection Reagent
Integration efficiency: ~25% under standard conditions
Duration of selection: 2 months
Price: 8 / 10 USD
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CRISPR Gene Editing: Designing the gRNA and Donor TemplatePiwi Matters Adaptation
Year: 2023
Region / city: Princeton, NJ
Topic: Gene editing, CRISPR/Cas9
Document type: Educational project
Organization / institution: Princeton University
Author: Laurel Lorenz
Target audience: Undergraduate students, molecular biology researchers
Period of validity: N/A
Approval date: N/A
Date of changes: N/A
CRISPR Bombshell: Chinese Researcher Claims Creation of Gene-Edited Twins Resistant to HIV, November 2018
Year: 2018
Date of publication: November 26, 2018
Location: Hong Kong, China
Topic: Human genome editing and bioethics
Document type: News article
Publication: Science
Author: Dennis Normile
Individuals mentioned: He Jiankui; Anthony Fauci; Pablo Tebas; Julian Savulescu; Sarah Chan; Jennifer Doudna; Richard Hynes; Alta Charo
Organizations: Southern University of Science and Technology; Chinese Society for Cell Biology; National Institute of Allergy and Infectious Diseases; University of Pennsylvania; University of Oxford; University of Edinburgh; University of California, Berkeley; National Academies of Sciences, Engineering, and Medicine; Nuffield Council on Bioethics
Scientific technique: CRISPR-Cas9 genome editing
Biological target: CCR5 gene in human embryos
Context event: International Summit on Human Genome Editing
Subjects of research: Gene-edited twin girls referred to as Lula and Nana
Research aim stated: Introduction of CCR5 mutation associated with resistance to HIV infection
BME Undergraduate Tracking Document
Year: 2021
Region / City: Not specified
Subject: Biomaterials, Biomedical Engineering
Document Type: Educational Program Guide
Institution: WPI
Author: Not specified
Target Audience: Undergraduate students
Period of Validity: Not specified
Approval Date: Not specified
Date of Changes: Not specified
Multivariate Analyses in Biostatistics - BME 50100
Year: 2025
Region / City: West Lafayette, Indiana
Topic: Biostatistics, Multivariate Analysis, Biomedical Engineering
Document Type: Course Syllabus
Institution: Purdue University
Instructor: Young Kim
Target Audience: Graduate students
Prerequisite: IE 330 Probability and Statistics in Engineering II or equivalent
Start Date: Fall 2025
End Date: N/A
Approval Date: N/A
Last Updated: N/A
BME Undergraduate Tracking Document
Year: 2023
Region / City: N/A
Field: Biomedical Engineering
Document Type: Academic Tracking Document
Organization / Institution: N/A
Author: N/A
Target Audience: Students in Biomedical Engineering
Period of Validity: N/A
Approval Date: N/A
Date of Changes: May 22, 2023
BME 130 BIOMEDICAL SIGNALS AND SYSTEMS
Year: 2012
Region / city: Not specified
Subject: Biomedical Engineering, Signals and Systems
Document type: Course syllabus
Institution: Not specified
Author: Zoran Nenadic
Target audience: Students in Biomedical Engineering and Premedical majors
Period of validity: Fall 2012
Approval date: June 2012
Modification date: Not specified
Program Overview: During the 2024-25 admissions cycle, the BME program enrolled 42 students out of a pool of 1,235 applicants. In 2024, there were 299 total students enrolled in the program.
Year: 2024-2025
Region / City: East Baltimore, Homewood, Bayview campuses
Topic: Biomedical Engineering PhD Program Admissions
Document Type: Program Overview
Organization: Johns Hopkins University School of Medicine
Author: Not specified
Target Audience: Prospective PhD applicants
Period of Validity: 2024-2025 admissions cycle
Approval Date: Not specified
Date of Changes: Not specified
Report requirements for BME 304X Biomedical Engineering Research
Year: 2023
Region / City: Not specified
Subject: Biomedical Engineering Research
Document Type: Report Requirements
Organization / Institution: University of Texas at San Antonio
Author: Not specified
Target Audience: Students in BME 304X
Effective Period: Semester duration
Approval Date: Not specified
Modification Date: Not specified
Marshall University Syllabus: BME 101 Introduction to Biomedical Engineering (CRN 1131, SEC 101)
Year: 2023
Region / City: Huntington, WV
Subject: Biomedical Engineering
Document Type: Course Syllabus
Institution: Marshall University
Instructor: Dr. Prabir Patra
Target Audience: Undergraduate students in Biomedical Engineering
Course Duration: Fall 2023
Date Approved: Not specified
Date of Last Revision: Not specified
Proposal for consideration of approval of independent study towards undergraduate BME elective credit
Note: Year
Topic: Biomedical Engineering Education
Document Type: Proposal
Organization / institution: BME undergraduate curriculum committee
Target audience: Undergraduate students, BME faculty
Elective credit eligibility: YES
Contextual description: A proposal document for approval of an independent study course to count towards BME undergraduate elective credit, detailing course requirements, student outcomes, and assessment methods.
MUSIC EDUCATION, BME 4 - YEAR PLAN
Year: 2022
Note: Region / City
Topic: Music Education
Document Type: Academic Plan
Target Audience: Music Education Majors
Period of Validity: Entire Undergraduate Program
Description: A 4-year course plan for Music Education students, detailing specific course requirements and sequencing throughout their undergraduate studies.
Topics: piggyBac CRISPR BME