№ files_lp_4_process_1_42884
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Supplementary laboratory protocol presenting primer and probe sequences, reaction compositions, thermocycling parameters, plasmid constructs, and validation results for a real-time PCR panel targeting multiple intestinal protozoa in stool samples.
Year:
Not specified
Region / City:
Basel, Switzerland
Field:
Parasitology
Document Type:
Supplementary scientific protocol and validation data
Institution:
Swiss Tropical and Public Health Institute (SwissTPH)
Department:
National Reference Centre for Parasitology
Methodology:
Real-time PCR using TaqMan probe assays
Assay Format:
Six duplex real-time PCR reactions and one simplex reaction
Target Organisms:
Entamoeba dispar, Entamoeba histolytica, Cryptosporidium spp., Cryptosporidium hominis/parvum, Microsporidia (Encephalitozoon spp., Enterocytozoon bieneusi), Entamoeba moshkovskii, Entamoeba polecki, Dientamoeba fragilis, Giardia lamblia, Cyclospora cayetanensis, Cystoisospora belli, Blastocystis sp.
Gene Target:
18S rRNA gene
Sample Type:
Human stool samples
Validation Material:
Plasmids with sequence inserts and clinical samples
Analytical Sensitivity:
1–10 plasmids/µl depending on assay
Diagnostic Sensitivity:
Reported up to 100% for validated assays
Specificity:
100% for tested organisms during validation
Laboratory Method:
Quantitative real-time polymerase chain reaction (qPCR)
Thermocycling Profile:
50 °C for 2 min, 95 °C for 10 min, followed by 40 cycles of 95 °C for 15 s and 58 °C for 1 min
DNA Extraction Material:
Approximately 1 g stool aliquots stored at −80 °C prior to molecular analysis
Reagents:
GeneExpression Mastermix (Thermo Fisher Scientific), primers and TaqMan probes
Price: 8 / 10 USD
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Year:
2024
Region / city:
Australia
Document Type:
Medical Submission
Organization / Institution:
PBAC
Author:
Not specified
Target audience:
Medical practitioners, pharmaceutical authorities
Period of validity:
Not specified
Approval date:
Not specified
Amendment date:
November 2024
Note:
Context
Year:
2023
Region / city:
Australia
Document type:
Submission for drug listing
Institution:
TGA (Therapeutic Goods Administration)
Author:
STADA Pharmaceuticals Australia Pty Ltd
Target audience:
Medical practitioners, healthcare professionals, policymakers
Period of validity:
Not specified
Approval date:
August 29, 2023
Date of amendments:
Not specified
Clinical claim:
LECIG is non-inferior in terms of efficacy and safety compared to LCIG
Registration status:
TGA registered
Field:
Gastroenterology
Topic:
Metabolic endoscopy and type 2 diabetes treatment
Type of document:
Clinical study report
Study design:
First in-human extension study
Intervention:
Proximal Intestinal Mucosal Ablation (PIMA)
Preceding study registration:
NCT05887635
Medical condition:
Type 2 diabetes (T2D)
Primary outcomes:
Safety, tolerability, feasibility, efficacy
Sample size:
8 patients
Patient characteristics:
Uncontrolled T2D (HbA1c 7.5–10.0%) despite ≥1 glucose lowering agent
Procedure details:
Up to 75 cm post-ampullary mucosal ablation using second-generation through-the-scope RFVA catheter
Anaesthesia:
General anaesthesia
Follow-up period:
3 months
Key measurements:
HbA1c, HOMA-IR, BMI, adverse events, VAS pain score
Journal:
World Journal of Gastroenterology
Manuscript Number:
25986
Manuscript Type:
Minireview
Title Running:
Current understanding concerning ISCs
Authors:
Shuang Cui; Peng-Yu Chang
Affiliation:
Department of Radiation Oncology, The First Bethune Hospital of Jilin University, Changchun 130021, Jilin Province, China
Corresponding Author:
Peng-Yu Chang, MD
Funding:
National Natural Science Foundation of China (No. 81502751; No. 81372929)
Conflict of Interest:
None declared
Open Access License:
Creative Commons Attribution Non Commercial (CC BY-NC 4.0)
Manuscript Source:
Invited manuscript
Received Date:
March 27, 2016
Accepted Date:
June 15, 2016
Keywords:
Intestinal stem cell; Cell development; Colorectal cancer
Publisher:
Baishideng Publishing Group Inc.
Year:
2016
Authors:
Noelia Rodriguez Mier; Senne Cuyx; Kris De Boeck; Marijke Proesmans; Mieke Boon; Lieven Dupont; Kaline Arnauts; Elke De Wachter; Stephanie Van Biervliet; Monique Lequesne; Vicky Nowé; Hedwige Boboli; Anabela Santo Ramalho; François Vermeulen; on behalf of the Belgian Organoid Project
Affiliations:
KU Leuven; University Hospital Leuven; University Hospital Brussels; Ghent University Hospital; Antwerp University Hospital; GZA Hospital; CHR Citadelle
Country:
Belgium
Research field:
Cystic fibrosis; CFTR modulators; organoid research; personalized medicine
Study population:
60 people with cystic fibrosis homozygous for F508del
Genetic focus:
F508del CFTR mutation (homozygous)
Interventions studied:
Elexacaftor/tezacaftor/ivacaftor; Tezacaftor/ivacaftor
Methods:
Patient-derived intestinal organoids from rectal biopsies; forskolin-induced swelling assay; comparison with clinical outcomes
Clinical outcomes assessed:
Lung function; sweat chloride levels; body weight; pulmonary exacerbations
Type of document:
Scientific research article
Source:
PubMed
DOI / Identifier:
40883119
Subject:
Intestinal surgery and anastomosis techniques
Type of document:
Educational surgical reference text
Field:
Veterinary surgery
Species:
Cats and dogs
Surgical focus:
Small intestinal resection and anastomosis
Suture materials discussed:
Silk, Dacron, Nylon, Polypropylene, Chromic gut, Polyglycolic acid (Dexon), Polygalactin 910 (Vicryl), Polydioxanone (PDS), Polyglyconate (Maxon), Poliglecaprone (Monocryl), Biosyn
Stapling devices mentioned:
Auto Suture 35 skin stapler, GIA stapler, TA stapler
Key surgical considerations:
Suture selection, suture pattern, needle type, omental flap coverage, serosal patching
Complications addressed:
Leakage, peritonitis, short bowel syndrome, mucosal eversion
Anatomical focus:
Small intestine, mesenteric border, antimesenteric border, greater omentum
Authors:
Yan Xu; Yong-Li Dai; Hong Lei; Ying-Lun Yuan; Tian-Le Hu; Xia Wei; Lin-Mei Guo; Yong-Mei Lan
Corresponding Authors:
Lin-Mei Guo; Yong-Mei Lan
Study Period:
November 2024–March 2025
Location:
Lanzhou, Gansu, China
Type of Document:
Original research article
Field:
Paediatric medicine; Microbiology; Clinical immunology
Study Population:
Children aged 3–14 years
Sample Size:
90 participants (30 MPP; 30 NMP; 30 healthy controls)
Methods:
16S rRNA sequencing; serum trace element detection; clinical laboratory analysis
Ethics Approval:
Approved by hospital ethics committee (2024KY22213)
Keywords:
16S rRNA; children; iron; intestinal flora; Mycoplasma pneumoniae pneumonia; zinc
Document type:
Supplementary material
Components:
Supplementary Figures; Supplementary Table; Supplementary references
Figures included:
Figure S1; Figure S2; Figure S3; Figure S4
Table included:
Table S1
Research topic:
Gut microbiota, intestinal inflammation and ulcerative colitis in murine models
Experimental subjects:
Mice
Biological focus:
Colonic tissue; intestinal barrier function; immune response; gut microbiota at genus level
Analytical methods mentioned:
Western blot; immunoblot; densitometric analysis
Key elements:
SCR-CDs synthesis; organ weight measurement; bacterial genera classification as beneficial or harmful; associated pathological states
Referenced conditions:
Ulcerative colitis; inflammatory bowel disease; colitis
Cited works:
Peer-reviewed journal articles with DOIs listed
Year:
2023
Region / City:
Padova, Italy
Topic:
Pharmaceutical Development, Drug Solubility
Document Type:
Research Study
Organization / Institution:
University of Padova, GlaxoSmithKline
Author:
Marco Brendolan, Francesca Cenci, Konstantinos Stamatopoulos, Fabrizio Bezzo, Pierantonio Facco
Target Audience:
Pharmaceutical Researchers, Bioengineers
Period of Validity:
N/A
Approval Date:
N/A
Date of Changes:
N/A
Year:
2021
Region / City:
New York, NY, USA
Subject:
Endocrinology, Metabolism
Document Type:
Research Article
Organization / Institution:
Columbia University, University of Cambridge, Case Western Reserve University
Authors:
Rossana M. Calderon, Christopher A. Smith, Emily L. Miedzybrodzka, Josie A. Silvaroli, Marcin Golczak, Fiona M. Gribble, Frank Reimann, William S. Blaner
Target Audience:
Researchers, medical professionals, endocrinologists
Period of Action:
Ongoing
Approval Date:
2021-01-01
Date of Changes:
N/A
Keywords:
Retinol-binding protein 2, Retinoids, Enteroendocrine cells, GIP, GLP-1, Obesity, Glucose intolerance, Metabolic diseases
Abstract:
Retinol-binding protein 2 (RBP2) is essential for enteroendocrine cell function in the intestine, influencing the secretion of incretins such as GIP and GLP-1, and plays a critical role in metabolic processes like glucose homeostasis and obesity.
Year:
2026
Region / City:
Adelaide, SA
Subject:
Microbiome, Health, Biosecurity, Research Career Development
Document Type:
Job Description
Organization / Institution:
CSIRO
Author:
CSIRO Human Resources
Target Audience:
Australian/New Zealand Citizens, Australian Permanent Residents, Australian Temporary Residents
Effective Period:
3 years full-time
Approval Date:
Not specified
Modification Date:
Not specified
Year:
2022
Region / City:
Kolkata, India
Topic:
Intestinal parasitic co-infection, Segmental colitis
Document Type:
Case Report
Institution:
Institute of Post Graduate Medical Education & Research, SSKM Hospital, Kolkata
Authors:
Dr. Sanchari Bairagya, Dr. Dipankar Paul, Dr. Kumkum Bhattacharyya
Target Audience:
Medical professionals, researchers, students in microbiology and gastroenterology
Date of Approval:
2022
Date of Modification:
Not specified
Keywords:
Protozoa, Helminths, Co-infection, Segmental colitis, Giardia intestinalis, Hymenolepis nana, Strongyloides stercoralis
Year:
2026
Region / City:
Grand Forks, North Dakota, USA
Subject:
Clinical Case Report, Small Bowel Volvulus
Document Type:
Case Report
Institution:
University of North Dakota, School of Medicine & Health Sciences
Authors:
Henry Elsenpeter, Paxten Wahlund, Selly Strauch, Susan Roe
Corresponding Author:
Henry Elsenpeter
Target Audience:
Medical professionals, forensic pathologists
Period of Study:
2026
Investigation Type:
Medico-legal death investigation
Patient Age:
85 years
Patient Sex:
Female
Medical History:
Hypothyroidism
Cause of Death:
Small bowel volvulus with necrotic ileum
Key Findings:
Ileum necrosis, peritoneal hemorrhage, cecal and splenic adhesions
Confidentiality Level:
BL - Restricted for internal use
TC ID / Revision:
00153898/C
Document Status:
Document Released
Document No.:
N/A
WBS code:
5.2 - RP3 Particle acceleration by lasers
PBS code:
E.E4.ELMA.4.5.4
Project branch:
Engineering & Scientific documents (E&S)
Document Type:
Specification (SP)
Position:
Senior researcher
Prepared by:
Senior researcher Valentina Scuderi
Reviewed By:
Daniele Margarone, Ladislav Půst, Pavel Bastl, Roman Kuřátko, Tomáš Laštovička, Veronika Olšovcová, Viktor Fedosov
Approved by:
Georg Korn
Revision History / Change Log:
D. Margarone, V. Scuderi, A. Kuzmenko
Date of Creation:
26.07.2017
Date of Last Modification:
22.08.2017
Change Description:
RSD draft creation, RSD update, formal update for approval
Introduction:
The Requirements Specification Document (RSD) details technical requirements and constraints for the Real-time Digital Oscilloscope to be purchased for the ELI Beamlines project.
Purpose:
The document defines the functional, performance, safety, and quality requirements for the Oscilloscope.
Scope:
The Oscilloscope specifications include performance standards, interface needs, power supply compatibility, safety, and delivery requirements.
Terms, Definitions and Abbreviations:
AC, BW, CA, Ch, CPU, DC, ELI, GND, pts, RMS, RSD, USB, WXGA
References to standards:
This document permits alternatives to specified standards if they are equivalently proven by the supplier.
Functional, Performance and Design requirements:
Includes minimum specifications for bandwidth, vertical resolution, sample rate, memory, sensitivity, and accuracy of the Oscilloscope.
Interface requirements:
Specifies connectivity options such as Ethernet and USB, and software compatibility with Windows 7.
Power supply:
The Oscilloscope is compatible with a wide range of AC input voltages and has specified power consumption limits.
Delivery requirements:
Delivery of the Oscilloscope must be managed by the Supplier to the ELI Beamlines final destination.
Safety Requirements:
Supplier must provide a Declaration of Conformity or equivalent documentation for compliance with EU regulations.
Quality Requirements:
The Supplier must provide user manuals, calibration certificates, and proof of outgoing checks, as well as ensure non-conformance control and warranty repair procedures.
Year:
2012
Region / City:
United States
Topic:
Real-Time Location Systems (RTLS) for VA
Document Type:
Performance Work Statement
Agency:
Department of Veterans Affairs
Author:
Not specified
Target Audience:
Veterans Health Administration, VA personnel, contractors, and technology vendors
Period of Effect:
Not specified
Approval Date:
February 24, 2012
Modification Date:
Not specified
Note:
Year
Countries & Regions:
Asia Pacific, Southern and Eastern Africa, West and Central Africa, Eastern Mediterranean and Middle East and North Africa, Afghanistan, Bangladesh, Burkina Faso, Burundi, Cameroon, Ethiopia, Fiji, Indonesia, Lao PDR, Libya, Myanmar, Nepal, occupied Palestinian territory (oPt), Pakistan, Papua New Guinea, Philippines, Somalia, South Sudan, Sri Lanka, Sudan, Syria (cross-border operation from Gaziantep), Vanuatu, Yemen
Year:
2019
Region / City:
USA, Virginia
Topic:
Real-time video in medical imaging
Document Type:
Technical standard
Organization / Institution:
DICOM Standards Committee, NEMA
Author:
DICOM Standards Committee
Target Audience:
Medical professionals, healthcare technology developers
Effective Period:
Not specified
Approval Date:
September 16, 2019
Amendment Date:
Not specified
Organization:
DICOM Standards Committee
Working Group:
Working Group 13
Standard series:
NEMA Standards Publication PS 3
Supplement number:
202
Title:
Real-Time Video
Version:
Draft Letter Ballot
Date:
May 23–24, 2019
Status:
Draft document
Work item:
DICOM Workitem 2016-12-D
Location:
Rosslyn, Virginia, USA
Copyright holder:
NEMA
Copyright year:
2018
Scope:
Real-time transport, metadata, and representation of video and audio in DICOM
Related standards:
SMPTE ST 2110 series
Document type:
Technical standard supplement
Target domain:
Medical imaging and real-time clinical video
Informative and normative content:
Mixed
Year:
2022
Region / city:
Geneva
Topic:
Quality of Experience (QoE), 5G Networks, Multimedia Services
Document type:
Technical Report
Organization:
ITU-T
Author:
ITU-T Study Group 12
Target audience:
Telecommunications professionals, researchers, and engineers
Effective period:
2022
Approval date:
17 June 2022
Modification date:
N/A