№ files_lp_4_process_3_115807
File format: docx Character count: 3478 File size: 23 KB
Year: 2021
Institution: Niigata University
Type of document: Research protocol and experimental report
Target molecules: GlyR (GLRA1), GABABR (GABBR1), NMDAR, AMPAR, LGI1, GABAAR, CASPR2
Methodology: PCR amplification, In-Fusion HD cloning, cell-based assay (CBA), fluorescence microscopy
Sample type: Serum, cerebrospinal fluid (CSF)
Patient group: 55 SPS patients, neurodegenerative disorder controls, healthy volunteers
Assay validation: Control samples tested negative
Reference studies: Dalakas MC et al., Tanaka K et al., Nakane S et al., Nakanishi T et al., Maeda K et al., Iizuka T et al., Nanaura H et al.
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Il-17 Signaling in Primary Sclerosing Cholangitis Patient-Derived Organoids
Year: 2023
Region / City: N/A
Subject: Primary Sclerosing Cholangitis, IL-17 Signaling
Document Type: Research Article
Organization / Institution: Mayo Clinic
Author: Ana Sofia Garcia Moreno, Maria Eugenia Guicciardi, Alexander Q. Wixom, Erik Jessen, Jingchun Yang, Sumera I. Ilyas, Jackie K. Bianchi, Filippo Pinto e Vairo, Konstantinos N. Lazaridis, Gregory J. Gores
Target Audience: Researchers, Healthcare professionals, Scientific community
Period of Validity: N/A
Date of Approval: N/A
Date of Changes: N/A
Supplementary Data: Comparison of DNA and RNA Isolation Efficiency in A549 Cell Line, Patient-Derived Organoids, and Pleural Effusion Using Adapted QIAamp® DNA FFPE Tissue Kit
Year: 2023
Region / City: Not specified
Topic: DNA and RNA isolation protocols
Document Type: Supplementary Data
Author: Not specified
Target Audience: Researchers in molecular biology and genomics
Period of Action: Not specified
Approval Date: Not specified
Date of Changes: Not specified
Correlation between patient-derived intestinal organoids and clinical responses to CFTR modulators in people with cystic fibrosis homozygous for F508del
Authors: Noelia Rodriguez Mier; Senne Cuyx; Kris De Boeck; Marijke Proesmans; Mieke Boon; Lieven Dupont; Kaline Arnauts; Elke De Wachter; Stephanie Van Biervliet; Monique Lequesne; Vicky Nowé; Hedwige Boboli; Anabela Santo Ramalho; François Vermeulen; on behalf of the Belgian Organoid Project
Affiliations: KU Leuven; University Hospital Leuven; University Hospital Brussels; Ghent University Hospital; Antwerp University Hospital; GZA Hospital; CHR Citadelle
Country: Belgium
Research field: Cystic fibrosis; CFTR modulators; organoid research; personalized medicine
Study population: 60 people with cystic fibrosis homozygous for F508del
Genetic focus: F508del CFTR mutation (homozygous)
Interventions studied: Elexacaftor/tezacaftor/ivacaftor; Tezacaftor/ivacaftor
Methods: Patient-derived intestinal organoids from rectal biopsies; forskolin-induced swelling assay; comparison with clinical outcomes
Clinical outcomes assessed: Lung function; sweat chloride levels; body weight; pulmonary exacerbations
Type of document: Scientific research article
Source: PubMed
DOI / Identifier: 40883119
Supplement for A pan-cancer patient-derived xenograft histology image repository with genomic and pathologic annotations enables deep learning analysis
Year: 2022
Region / City: Not specified
Topic: Cancer research, genomics, histology
Document Type: Supplementary information
Organization / Institution: The Jackson Laboratory for Genomic Medicine, Bioinformatics Institute (BII), Agency for Science, Technology and Research (A*STAR), and others
Author: Brian S White, Xing Yi Woo, Soner Koc, Todd Sheridan, Steven B Neuhauser, et al.
Target Audience: Researchers, clinicians, and institutions in genomics and cancer research
Period of validity: Not specified
Approval Date: Not specified
Date of Changes: Not specified
Contextual description: Supplementary information for a genomic and histological image repository enabling deep learning analysis in cancer research.
Generation and analysis of transgenic mice and patient-derived xenografts for Atoh1 studies
Year: 2026
Region / Institution: University of Oregon, Sanford Research, Institut Curie, Necker Hospital, Hospital for Sick Children
Field: Molecular biology / Neuro-oncology
Document type: Research supplemental materials and methods
Organ / Institution: Transgenic Mouse Facility, Sanford Research, Institut Curie, Necker Hospital
Authors: Not specified
Target audience: Researchers in genetics and oncology
Experimental models: Transgenic mice, ES cells, patient-derived xenografts (PDX)
Animal procedures approval: Sanford Research IACUC, Institut Curie ethical committee IDFParis-Comité 1 (#2011-0012)
Human research approval: Institutional Review Board at Necker Hospital, Research Ethics Board at the Hospital for Sick Children
Techniques used: DNA constructs, transgenic mouse generation, orthotopic transplantation, bioluminescence imaging, NanoString gene expression profiling, DNA methylation analysis
Drugs / treatments: Tamoxifen, vismodegib
Mouse strains: C57BL/6J, B6.Cg-Tg(Atoh1-cre)1Bfri/J, Tg(Atoh1-cre/Esr1*)14Fsh/J, B6N.129-Ptch1tm1Hahn/J, C57BL/6-Tg(Neurod2-Smo*A1)199Jols/J, NOD.Cg-Prkdc Il2rg/SzJ, athymic nude, Tg(Lmx1a-cre)1Kjmi
Cell lines: JM8.N4 ES cells
Topics: GlyR GABAB Patient-Derived