№ files_lp_3_process_7_096958
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Quantitative supplementary dataset presenting RT-PCR cycle threshold values, viral titers, replicate analyses, and recovery efficiency measurements supporting experimental evaluation of a rapid viability RT-PCR method for detecting infectious SARS-CoV-2 from swab samples.
Document Type: Supplementary data appendix
Subject: Development and validation of a rapid viability RT-PCR (RV-RT-PCR) method for detection of infectious SARS-CoV-2
Pathogen: SARS-CoV-2
Experimental Model: Vero E6 cells
Institutions: U.S. Environmental Protection Agency, Office of Research and Development; Lawrence Livermore National Laboratory
Authors: Sanjiv R. Shah; Staci R. Kane; Maher Elsheikh; Teneile M. Alfaro
Geographic Location: Washington, District of Columbia, USA; Livermore, California, USA
Data Type: RT-PCR cycle threshold (CT) measurements; plaque forming units (PFU); TCID50 values; recovery efficiency data
Infection Conditions: 1-hour and 2-hour infection periods with post-infection incubation at T0, T12, and T24
Sample Type: SARS-CoV-2-infected cell cultures and swab swatches
Analytical Replicates: Triplicate RT-PCR analyses with pooled standard deviations
Tables Included: Table S1; Table S2; Table S3; Table S4
Price: 8 / 10 USD
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Primer Design for RT-PCR: Guidelines and Tips
Note: Year
Topic: Primer Design, RT-PCR
Document Type: Guidelines
Rubella genotyping RT-PCR 2-fragment, Invitrogen (CDC)RT-PCR protocol for rubella genotyping (2 Fragment System)
Year: 2012
Region / city: CDC
Topic: Molecular Epidemiology, Genotyping
Document type: Protocol
Organization: CDC
Author: CDC
Target audience: Laboratories, Research Institutions
Period of validity: N/A
Approval date: 03/06/2012
Date of amendments: N/A
Purpose: Amplification of 2 fragments from the rubella virus (RuV) envelope protein 1 (E1) coding region
PCR product sizes: Fragment 1 (480 nts), Fragment 2 (633 nts)
Required equipment: Thermocycler, Micropipettors, Class II biological safety cabinet
RNA preparation: Extraction from clinical samples or cell cultures
Primers: RV8633, RV8945, RV9112, RV9577
Control RNA: Dried rubella RNA control
Handling instructions: Store primers and control RNA at specified temperatures, prepare working solutions before use
Safety recommendations: Use dedicated equipment and workspaces for RNA procedures
Contextual description: Protocol for Rubella virus genotyping using RT-PCR to amplify specific RNA fragments, primarily for molecular epidemiological research.
Accessing the Saved Property to Detect Document Changes in Microsoft Word
Topic: Microsoft Word automation
Type of document: Technical knowledge base article
Software: Microsoft Word
Programming language: VBA
Subject: Document change detection
Concepts discussed: Saved property, Document_Close event, Save and SaveAs methods
Audience: Developers and advanced users working with Word macros
Focus: Version control logic in documents
Technical context: Word object model
Using Intelligent Liver Function Tests (iLFTs) to detect malignancy: a retrospective observational cohort study
Year: 2022
Region / City: NHS Tayside
Topic: Liver disease detection, Malignancy detection
Document Type: Retrospective Observational Cohort Study
Institution: NHS Tayside
Author: Not specified
Target Audience: Clinicians, Medical researchers
Period of Effect: April 2022 – July 2024
Date of Approval: Not specified
Date of Changes: Not specified
2022-2023 Proposal for Validation of HPLC and MS Methods to Detect NNAT in Biological Samples
Year: 2022-2023
Region / Institution: University of Nebraska–Lincoln, Water Sciences Laboratory, USA
Subject: Analytical chemistry, toxicology
Document type: Research proposal
Authors: Unspecified student researcher under Drs. Kim, Peeples, Rhoades, and Snow
Target audience: Scientific community and laboratory researchers
Research focus: Detection and validation of N-nitrosoatrazine (NNAT) in blood and tissue samples
Methods: High performance liquid chromatography (HPLC) and mass spectrometry (MS)
Sample types: Mouse placenta, fetal tissue, rat blood
Timeline: Stage 1 (Jan–May 2022), Stage 2 (Jun 2022–May 2023)
Objective: Assess accuracy and efficiency of HPLC and MS for NNAT detection
Topics: RV-RT-PCR RT-PCR Detect