№ files_lp_3_process_7_105488
File format: docx Character count: 30434 File size: 807 KB
Year: 2023
Region / city: West Hills, CA, United States
Topic: Kidney allograft rejection monitoring
Document type: Research article
Organization / institution: Thermo Fisher Scientific
Authors: McFaul M, Ventura C, Evans S, Dundar H, Rumpler MJ, McCloskey C, Lowe D, Vlassov AV
Target audience: Researchers, clinicians in organ transplant and diagnostics
Period of validity: 2023
Date of approval: October 23, 2023
Date of changes: None
Keywords: Kidney allograft, Post-transplant monitoring, Liquid biopsy, Exosome, mRNA, Quantitative polymerase chain reaction
Corresponding author: Alexandre V Vlassov, PhD
Summary: Research on the stability of mRNA from urine-derived exosomes for kidney allograft rejection detection, including effects of sample transportation, storage, and interference substances.
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Supplementary Methods for Cell Culture, Blood Exosome Isolation, and Exos-Met/sicPLA2 Characterization in Glioblastoma Cell Experiments
Document Type: Supplementary Methods
Scientific Field: Molecular Biology; Cancer Biology; Nanomedicine
Research Focus: Exosome-mediated delivery of metformin and siRNA in glioblastoma cells
Biological Materials: Primary GBM cells (N9, TBD0220); murine brain endothelial cells (bEnd.3); GBM cell lines (GL261, CT2A); neuronal cells (HT22); astrocyte cells (C8-DIA)
Key Molecules: PTRF protein; cPLA2; metformin; siRNA (siPTRF, chol-sicPLA2)
Experimental Techniques: Cell culture; lentiviral transfection; siRNA transfection; ultracentrifugation; western blot analysis; confocal laser scanning microscopy; flow cytometry; FRAP membrane fluidity assay; electroporation; nanoparticle tracking analysis; transmission electron microscopy; dynamic light scattering; high-performance liquid chromatography; Seahorse extracellular flux analysis
Biological Structures Studied: Blood-derived exosomes
Analytical Software: ImageJ
Laboratory Equipment: CLSM (FV1200 Olympus); NanoSight NS300; JEM-2100F TEM; Bio-Rad Gene Pulser Xcell Electroporation System; Seahorse XF24 Analyzer
Chemical Reagents: fetal bovine serum; metformin; DiI dye; Cy5 dye; oligomycin; FCCP; rotenone; antimycin A
Purpose of Experiments: Investigation of cellular uptake, membrane fluidity, mitochondrial function, and delivery efficiency of engineered exosomes in GBM cells
Geographical Reference of Reagents: Shanghai, China; Suzhou, China; Beijing, China; USA
Royal Matrix HD® Allograft Dermis
Note: Year
Cryopreservation and Packaging Procedure for Allograft Skin Following Antibiotic Disinfection
Year: 2026
Region / City: Not specified
Subject: Tissue Banking / Allograft Skin Processing
Document Type: Standard Operating Procedure
Organization / Institution: Tissue Bank Laboratory
Author: Not specified
Target Audience: Laboratory Technologists
Effective Period: Until further notice
Approval Date: Not specified
Revision Date: Not specified
Materials Used: Antibiotic solution, Lactated Ringer’s, cryoprotectant solution (10% dimethyl sulfoxide in X-Vivo 10™), sterile pouches, freezing materials, LN2 protective equipment
Safety Measures: Use of sterile gown, gloves, mask, and caps; controlled biosafety cabinet environment; LN2 handling precautions
Quality Control: Post-antibiotic culture testing, verification by two technologists, single donor processing, sterile indicator checks
Procedure Limitations: Maximum 21 grafts per control rate freezer per session
Related Documents: TBK series SOPs referenced for cabinet operation, freezing solution, labeling, and data entry
Laboratory Environment: Biologically and temperature controlled, aseptic techniques enforced
Cryopreservation Duration: 5-year expiration from freezing date
Storage: Vapour phase of Quarantine liquid nitrogen tank, numbered racks
Laboratory Record-Keeping: Donor ID, culture labels, processing log, and data entry forms maintained
Flowcytometry and PET-CT analysis of kidney allograft rejection correlations
Year: 2026
Region / City: Not specified
Subject: Kidney transplant immunology
Document Type: Supplementary information
Institution: Academic research study
Author: Not specified
Study Population: Kidney transplant patients
Methods: Flowcytometry, PET-CT imaging
Variables: Peripheral T cell subsets, regulatory T cells, cortical and medullary uptake values, histopathological features
Sample Size: n = 11 for clinical correlations, n = 9 for histological correlations
Statistical Analysis: Wilcoxon Rank Sum Test, Spearman correlation
Significance Threshold: p < 0.05; trend p < 0.1
ACL Allograft Reconstruction Rehabilitation Protocol
Year: 2026
Type of document: Medical rehabilitation protocol
Body / institution: Orthopedic surgery department
Target audience: Patients undergoing ACL allograft reconstruction
Frequency: 1-4 times per week
Duration: 6-9 months
Surgery date: Specified per patient
Phases: 0-2 weeks, 2-6 weeks, 7-12 weeks, 3-6 months, 6-9 months
Exercises included: Range of motion, strengthening, proprioception, gait normalization, sport-specific agility
Restrictions: Avoid tibial rotation, limit brace use according to control
Topics: mRNA-based exosome Allograft