№ files_lp_4_process_2_87755
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Step-by-step laboratory protocol for cryopreserving allograft skin including rinsing, antibiotic testing, packaging in sterile conditions, and controlled freezing with detailed safety and quality measures.
Year:
2026
Region / City:
Not specified
Subject:
Tissue Banking / Allograft Skin Processing
Document Type:
Standard Operating Procedure
Organization / Institution:
Tissue Bank Laboratory
Author:
Not specified
Target Audience:
Laboratory Technologists
Effective Period:
Until further notice
Approval Date:
Not specified
Revision Date:
Not specified
Materials Used:
Antibiotic solution, Lactated Ringer’s, cryoprotectant solution (10% dimethyl sulfoxide in X-Vivo 10™), sterile pouches, freezing materials, LN2 protective equipment
Safety Measures:
Use of sterile gown, gloves, mask, and caps; controlled biosafety cabinet environment; LN2 handling precautions
Quality Control:
Post-antibiotic culture testing, verification by two technologists, single donor processing, sterile indicator checks
Procedure Limitations:
Maximum 21 grafts per control rate freezer per session
Related Documents:
TBK series SOPs referenced for cabinet operation, freezing solution, labeling, and data entry
Laboratory Environment:
Biologically and temperature controlled, aseptic techniques enforced
Cryopreservation Duration:
5-year expiration from freezing date
Storage:
Vapour phase of Quarantine liquid nitrogen tank, numbered racks
Laboratory Record-Keeping:
Donor ID, culture labels, processing log, and data entry forms maintained
Price: 8 / 10 USD
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The product description is provided for reference. Actual content and formatting may differ slightly.
Year:
2019
Region / City:
United States
Subject:
Cryopreservation storage for Veterans
Document Type:
Performance Work Statement (PWS)
Organization:
Department of Veterans Affairs (VA)
Author:
Not specified
Target Audience:
VA contractors and stakeholders
Period of validity:
Ongoing
Approval Date:
March 2019
Date of Changes:
Not specified
Note:
Year
Year:
2023
Region / city:
West Hills, CA, United States
Topic:
Kidney allograft rejection monitoring
Document type:
Research article
Organization / institution:
Thermo Fisher Scientific
Authors:
McFaul M, Ventura C, Evans S, Dundar H, Rumpler MJ, McCloskey C, Lowe D, Vlassov AV
Target audience:
Researchers, clinicians in organ transplant and diagnostics
Period of validity:
2023
Date of approval:
October 23, 2023
Date of changes:
None
Keywords:
Kidney allograft, Post-transplant monitoring, Liquid biopsy, Exosome, mRNA, Quantitative polymerase chain reaction
Corresponding author:
Alexandre V Vlassov, PhD
Summary:
Research on the stability of mRNA from urine-derived exosomes for kidney allograft rejection detection, including effects of sample transportation, storage, and interference substances.
Year:
2026
Region / City:
Not specified
Subject:
Kidney transplant immunology
Document Type:
Supplementary information
Institution:
Academic research study
Author:
Not specified
Study Population:
Kidney transplant patients
Methods:
Flowcytometry, PET-CT imaging
Variables:
Peripheral T cell subsets, regulatory T cells, cortical and medullary uptake values, histopathological features
Sample Size:
n = 11 for clinical correlations, n = 9 for histological correlations
Statistical Analysis:
Wilcoxon Rank Sum Test, Spearman correlation
Significance Threshold:
p < 0.05; trend p < 0.1
Year:
2026
Type of document:
Medical rehabilitation protocol
Body / institution:
Orthopedic surgery department
Target audience:
Patients undergoing ACL allograft reconstruction
Frequency:
1-4 times per week
Duration:
6-9 months
Surgery date:
Specified per patient
Phases:
0-2 weeks, 2-6 weeks, 7-12 weeks, 3-6 months, 6-9 months
Exercises included:
Range of motion, strengthening, proprioception, gait normalization, sport-specific agility
Restrictions:
Avoid tibial rotation, limit brace use according to control
Year:
Unknown
Region / City:
Unknown
Topic:
Healthcare, Ventilator Management
Document Type:
Checklist
Organization / Institution:
Unknown
Author:
Unknown
Target Audience:
Healthcare staff involved in ventilator care
Effective Period:
Unknown
Approval Date:
Unknown
Date of Changes:
Unknown
Year:
2020
Region / City:
Michigan
Topic:
Bleach Dilution for Disinfection
Document Type:
Standard Operating Procedure (SOP)
Institution:
Michigan State University, Center for Research on Ingredient Safety
Author:
Anderson, E., and J. Li
Target Audience:
Laboratory Personnel
Effective Date:
5-14-2020
Revision Date:
5-14-2020
Year:
Not specified
Region / City:
Not specified
Topic:
Potable water disinfection
Document type:
Product specification
Agency / Organization:
Not specified
Author:
Not specified
Target audience:
Industry professionals
Validity period:
Not specified
Approval date:
Not specified
Amendment date:
Not specified
Year:
2006
Region / City:
England, Scotland, Wales
Subject:
Avian Influenza, Animal Health, Cleansing and Disinfection
Document Type:
Legislative Order
Organization:
Department for Environment, Food and Rural Affairs, Scottish Government, Welsh Government
Author:
Department for Environment, Food and Rural Affairs, Scottish Government, Welsh Government
Target Audience:
Owners and agents responsible for cleansing and disinfection of premises affected by avian influenza
Period of Action:
2006 and ongoing
Approval Date:
2006
Date of Amendments:
Ongoing
Contextual Description:
A legislative order detailing procedures for cleansing and disinfection of premises affected by avian influenza in England, Scotland, and Wales.
Year:
N/A
Region / City:
N/A
Theme:
Infection Control, Cleaning and Disinfection
Document Type:
Policy
Organization / Institution:
N/A
Author:
N/A
Target Audience:
Staff and volunteers in centers
Effective Date:
N/A
Revision Date:
N/A
Approval Date:
N/A
Changes Date:
N/A
Year:
Not specified
Region / State:
Multiple U.S. states including AK, AR, CT, IA, KS, MI, NC, NJ, OK, PA, VA, WA, LA
Topic:
Tracer studies for water treatment verification
Document Type:
Meeting notes / discussion summary
Organization:
EPA and state water authorities
Participants:
Bill Davis, Nancy Feagin, Darlene Galido, Robert Reaves, Mandy Smith, Bob Campbell, Ben Haring, Todd Stephenson, Kaitlyn Bates, Emily Lester, Joseph McNally, Stephanie Stoner, Candy Thompson, David Dawson, Michele Welsh, Mark Sceery, Andrea Traviglia, Jason Gambatese
Objectives:
Discuss methods, contractor qualifications, flow rates, and data integrity for tracer studies in surface and groundwater systems under direct surface water influence
Regulatory Reference:
SWTR (Surface Water Treatment Rule)
Focus Contaminants:
Giardia, viruses
Discussion Date:
Not specified
Year:
Not specified
Region / City:
New York, USA
Subject:
Water Storage Tank Disinfection
Document Type:
Technical Specification
Organization / Agency:
New York State Department of Health
Reference Standards:
AWWA C652 - Disinfection of Water-Storage Facilities
Related Sections:
330110.58, 331616, 331636
Target Audience:
Contractors, water utility personnel
Submission Requirements:
Disinfection procedure, manufacturer’s certificate, test reports, qualifications statement
Field Quality Control:
Bacteriological testing, residual chlorine tests, sampling procedures
Safety Requirements:
Compliance with federal and state confined space regulations
Disposal Requirements:
Neutralization and safe discharge of disinfectant solutions
Year:
2024
Region / City:
New South Wales, Australia
Topic:
Healthcare-associated infections, cleaning and disinfection of medical equipment
Document Type:
Research article
Organization / Institution:
Avondale University, Queensland University of Technology, Monash University, Cabrini Health, and others
Authors:
Katrina Browne, Nicole M White, Philip L Russo, Allen C Cheng, Andrew J Stewardson, Georgia Matterson, Peta E Tehan, Kirsty Graham, Maham Amin, Maria Northcote, Martin Kiernan, Jennie King, David Brain, Brett G Mitchell
Target Audience:
Healthcare professionals, medical researchers
Period of Study:
March 20, 2023 - November 24, 2023
Approval Date:
Published online
Date of Changes:
None
Document type:
Risk assessment template collection
Subject:
Ultrasound probe reprocessing and use in medical procedures
Risk categories:
Patient safety, Operator safety, Environmental risk
Processes covered:
Cleaning, Disinfection/Sterilisation, Storage, Transport, Clinical use
Includes:
Risk matrix for likelihood and severity rating
Intended users:
Healthcare facilities and clinical departments
Applies to:
Ultrasound probes and associated reprocessing workflows
Fields included:
Institution, Department, Document Number, Written by, Approved by, Date, Next review due
Mitigation focus:
Reduction of identified hazards to low risk rating
Reference to standards:
Manufacturer Instructions for Use (IFU), Infection Prevention and Control guidance
Year:
2015
Region / City:
Texas, USA
Subject:
Water treatment, chloramine disinfection
Document Type:
Construction checklist
Organization:
Texas Commission on Environmental Quality
Author:
Water Supply Division, TCEQ
Target Audience:
Public water system operators, licensed professional engineers
Plan Review Team:
MC-159
TCEQ Log No.:
P-_____________________________
Public Water System I.D. No.:
__________________
Date of Issue:
2015
Regulations Referenced:
30 TAC Chapter 290
Chemical Standards:
ANSI/NSF Standard 60
Year:
2021
Region / City:
United States
Subject:
Cleaning and Disinfection Protocols
Document Type:
Technical Requirements
Organization:
Centers for Disease Control and Prevention (CDC), Federal Institute for Occupational Safety and Health (BAuA), Robert Koch Institute (RKI)
Author:
Unspecified
Target Audience:
Contractors and Facility Management Personnel
Period of Validity:
Indefinite, subject to updates
Approval Date:
Not specified
Date of Changes:
Not specified
Required Experience:
Experience in microbial-related remediation (crime scene cleanup, mold abatement, etc.)
Type of Disinfectants:
EPA-registered products effective against SARS-CoV-2 or equivalent
Personal Protective Equipment (PPE):
Required for contractors performing cleaning and disinfection
Cleaning Standards:
Detailed guidelines on cleaning and disinfecting high-contact surfaces
Access to US Facilities:
Contractors must submit documents for facility access
Year:
2023
Region / City:
Norton, Massachusetts
Theme:
Drinking water quality, contamination, health risks
Document type:
Public notice
Organization / Institution:
Norton Water Department
Author:
Norton Water Department
Target audience:
Residents of Norton, pregnant women, young children
Effective period:
August 2023 - May 2024
Approval date:
7/8/2024
Date of changes:
N/A
Contact Information:
[email protected]
Note:
, 508-285-0280
Year:
Not specified
Region / City:
Not specified
Topic:
Infection control, hospital sanitation, multidrug-resistant microorganisms
Document Type:
Research Table
Institution / Organization:
Not specified
Author:
Not specified
Target Audience:
Researchers, healthcare professionals
Period of Validity:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Keywords:
MDR A. baumannii, ESBL E. coli, ESBL K. pneumoniae, CRE E. coli, MDR P. aeruginosa, PX-UV Disinfection