№ files_lp_4_process_3_120094
Educational genetics laboratory reference explaining expected gel electrophoresis band patterns after PCR and restriction enzyme digestion used to determine TAS2R38 genotypes associated with PTC tasting phenotypes.
Subject: PTC tasting genetics
Topic: PCR analysis and restriction enzyme digestion of TAS2R38 taster gene variants
Document type: Educational laboratory reference sheet
Field of study: Genetics
Method: Polymerase Chain Reaction (PCR) with restriction enzyme digestion and gel electrophoresis
Genotypes described: Homozygous taster (TT), heterozygous taster (Tt), homozygous non-taster (tt)
Observed DNA fragment sizes: 221 bp, 177 bp, 44 bp
Phenotypic trait: Ability to taste PTC
Laboratory technique: DNA fragment analysis by gel electrophoresis
Intended context: Genetics laboratory exercise on genotype–phenotype comparison
Price: 8 / 10 USD
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