№ files_lp_4_process_3_073849
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This document provides detailed methods for qRT-PCR, ChIP assays, and the use of primers and probes in the study of multiple myeloma cell lines, including various reagents and experimental setups.
Year: Not specified
Region / City: Not specified
Topic: Molecular biology, multiple myeloma research
Document Type: Research methodology, supplementary material
Institution: Not specified
Author: Not specified
Target Audience: Researchers in molecular biology, oncology
Period of Validity: Not specified
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Date of Modifications: Not specified
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Construction of RAD16-Deleted Yeast Strains and Genome-Wide Chromatin Analysis Using ChIP-seq and MNase-seq
Year: 2026
Organism: Saccharomyces cerevisiae
Gene Targeted: RAD16
Methods: Yeast strain construction, UV irradiation, chromatin preparation, ChIP-seq, MNase-seq, library preparation
Techniques: Lithium-acetate transformation, PCR, chromatin immunoprecipitation, micrococcal nuclease digestion, DNA sequencing library preparation
Authors: Reed et al., Yu, Teng, Kent, Powel
Sample Type: Yeast cells
Experimental Conditions: UV-C irradiation, formaldehyde crosslinking, MNase digestion
Storage: Glycerol stocks, snap-frozen cells
Sequencing Platforms: ChIP-seq libraries prepared according to Life Technologies protocol
Expression and chromatin accessibility analysis of HER2 in SKBR3 and SKBR3-L cells
Year: 2026
Region / city: Not specified
Subject: Molecular biology, cell signaling
Document type: Supplementary figure description
Institution: Not specified
Author: Not specified
Cell lines: SKBR3, SKBR3-L
Experimental techniques: Fluorescence microscopy, imageJ analysis, chromatin accessibility assay, MA plot analysis
Statistical analysis: Unpaired t-test with Welch’s correction, p < 0.0001, adjusted p < 0.05
Magnification: 20x
Scale bars: 50 μm
Data points: n = 79 for parental cells, n = 31 for lapatinib-resistant cells
Target protein: HER2
Genomic features: Transcription start sites (TSS), nucleosome positioning
Topics: Taqman Immunoprecipitation Chromatin