№ lp_2_3_28182
Supplementary scientific material presenting strain and plasmid information, primer sequences for mutagenesis, crystallographic data for YojK (PDB 7VM0), enzymatic characterization, and spectroscopic analyses of Reb D.
Section: Supplementary Material
Content: Supplementary Figures and Tables
Supplementary Tables: 3
Supplementary Figures: 12
Main Protein: YojK
PDB code: 7VM0
Host strain: Escherichia coli BL21(DE3)
Plasmids: pET-21b(+), pACYCDuet-1
Genes: YojK, AtSuSy
Compounds analyzed: Reb A, Reb D
Analytical methods: LC-MS, HRMS (ESI-), 1H NMR, 13C NMR, COSY, TOCSY, HSQC, HMBC, ROESY, SDS-PAGE
Study focus: Glycosyltransferase mutagenesis, protein expression, enzymatic characterization, crystallographic analysis
Organisms referenced: Bacillus subtilis 168, Oryza sativa, Stevia rebaudiana, Vitis vinifera, Streptomyces antibioticus, Solanum lycopersicum
Price: 8 / 10 USD
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