№ files_lp_3_process_9_60352
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Detailed instructions for performing a transfection using Polyethylenimine (PEI), including preparation of stock solutions, transfection conditions, and medium composition for cell culture.
Year:
N/A
Region / City:
N/A
Subject:
Transfection Protocol
Document Type:
Scientific Protocol
Institution:
Polysciences
Author:
N/A
Target Audience:
Researchers
Duration:
N/A
Approval Date:
N/A
Modification Date:
N/A
Price: 8 / 10 USD
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The product description is provided for reference. Actual content and formatting may differ slightly.
Document type:
Supplementary material
Subject area:
Molecular biology and cancer research
Biological focus:
MIR22HG overexpression in cervical cancer cell lines
Cell lines:
HeLa; SiHa; C-33 A (HPV-)
Experimental methods:
Cell culture; transient transfection; RNA extraction; qRT-PCR; CCK-8 assay; Transwell migration and invasion assays
Genes referenced:
MIR22HG; GAPDH
Reagents and materials:
DMEM; MEM; FBS; Lipofectamine 2000; TRIzol; SYBR Green PCR kit; Matrigel
Data included:
Figure S1; Table S1; Table S7
Dataset description:
Annotated list of 4072 lncRNAs from HGNC database
Vendors mentioned:
Keygen Biotech (Nanjing, China); GeneChem (Shanghai, China); HyClone (Logan, UT, USA); Invitrogen (Carlsbad, CA, USA); Toyobo (Osaka, Japan); Beyotime (Shanghai, China); BD (Franklin Lakes, NJ, USA); Corning (New York, USA)
Year:
2026
Region / City:
Not specified
Subject:
Lipid nanoparticles, gene delivery, T cell transfection
Document type:
Research supplementary material
Institution:
Not specified
Author:
Not specified
Target audience:
Researchers in molecular biology and gene therapy
Experimental system:
Jurkat cells
Analyzed molecules:
DOPE, Cholesterol, C12-200, C14-PEG 2000, CAR-CD19BBz, ZsGreen pDNA
Methods:
Microfluidic mixing, electroporation, Lipofectamine comparison
Measured parameters:
Hydrodynamic diameter, polydispersity index, zeta potential, cell viability, transfection efficiency
Figures included:
S1–S5
Statistical analysis:
One-way ANOVA, unpaired t test
Note:
Year
Document Type:
Timesheet
Contextual description:
A document detailing timesheet guidelines for various speech competition categories, outlining penalties for exceeding time limits or using prohibited aids.
Note:
Year
Theme:
Program Evaluation
Document Type:
Self-Study
Context:
A self-study document detailing the processes and standards for assessing and improving the program evaluation system within an organization, including supporting documents and required attachments for assessment.
Note:
Year
Year:
20xx
Region / city:
Singapore
Subject:
Education, PEI (Private Education Institution)
Document type:
Report
Organization / institution:
PEI
Author:
Not specified
Target audience:
Not specified
Period of validity:
Not specified
Approval date:
dd/mm/yy
Date of changes:
Not specified
Year:
20xx
Region / City:
Not specified
Subject:
Education, Institution Profile
Document Type:
Institutional Profile
Organization / Institution:
PEI
Author:
Not specified
Target Audience:
Not specified
Period of Validity:
Not specified
Approval Date:
Not specified
Amendment Date:
Not specified
Note:
Year
Year:
2020
Date:
September 21, 2020
Location:
Royalty Centre, Prince Edward Island
Organization:
PEI 55+ Games Society
Document type:
Annual General Meeting agenda and organizational reports
Main sections:
Meeting agenda, President’s Report, 2020 Vogue Optical PEI 55 Plus Winter Games Host Committee Final Report
Events discussed:
2019 Summer Games, 2020 Winter Games, 2020 Regional Games, Canada 55 Plus Games
Author:
Kim Knight-Picketts (President’s Report); Summerside Winter Games organizing staff
Topic:
Organizational governance, sports events for adults aged 55+, financial and operational activities
Audience:
Members and voting participants of the PEI 55+ Games Society
Geographic scope:
Prince Edward Island, Canada
Related locations:
Summerside, Charlottetown, Stratford, Cornwall, Kensington
Sponsors mentioned:
Vogue Optical
Notable context:
Operational disruptions and event postponements related to the COVID-19 pandemic
Year:
2023
Region / Organization:
Health PEI
Document Type:
Standard Operating Procedure (SOP)
Owner:
Director of Talent Management
Effective Date:
February 20, 2023
Next Review Date:
February 20, 2024
Revision Dates:
None
Scope:
All areas within Health PEI
Purpose:
Outline the procedure to execute a system expression of interest to fill vacant shifts
Related Documents:
HR policies, collective agreements
Responsible Roles:
Manager, Human Resources Manager, Executive Assistant to the ED of Human Resources
Approval Authority:
Executive Director of Human Resources
Exceptions:
Not applicable
Year:
2024
Region / city:
Edinburgh, UK
Topic:
Genetics of Mortality in Critical Care
Document Type:
Study Protocol
Organization / Institution:
University of Edinburgh, NHS Lothian Health Board
Author:
Prof JK Baillie, Ms F Griffiths, Dr S Clohisey, Dr A Law, Dr N Parkinson, Mr L Murphy, Mr Edward Rippingale-Combes
Target Audience:
Researchers, Healthcare professionals
Duration:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Year:
Not specified
Region / City:
Cincinnati
Subject:
Social and Behavioral Sciences Research
Document Type:
Research Protocol Submission Guidelines
Institution / Organization:
University of Cincinnati
Author:
Not specified
Target Audience:
Researchers submitting to the IRB-S
Validity Period:
Not specified
Approval Date:
Not specified
Modification Date:
Not specified
Year:
2023
Region / City:
United States
Theme:
Racial and gender discrimination, military climate, sexual harassment, sexual assault
Document Type:
Research Protocol
Organization / Institution:
Department of Defense, Office of People Analytics (OPA)
Author:
Unknown
Target Audience:
Racial/ethnic minority service members in the U.S. military
Period of Action:
2023
Approval Date:
Unknown
Date of Changes:
Unknown
Version:
1.5.0.0
Protocol Posting Date:
June 2024
CAP Laboratory Accreditation Program Protocol Required Use Date:
March 2025
Authors:
Gulisa Turashvili, MD, PhD; Uma G. Krishnamurti, MD, PhD; Barbara A. Crothers, DO; Giovanna A. Giannico, MD; Krisztina Hanley, MD; Anna Plotkin, MD; Anthony N. Karnezis, MD, PhD.
Note:
Accreditation Requirements
Date of Protocol Posting:
June 2024
Date of Required Use:
March 2025
Year:
2025
Date:
September 11, 2025
Version:
v1.1
Document type:
Research report
Author:
Edgar Marcelo Quiroz Robles
Affiliation:
Independent Researcher
Field:
Theoretical cosmology and mathematical physics
Framework:
4-Dimensional Elastic Model of the Universe (4DEM)
Related theories:
String theory
Status:
Draft
Repository:
Zenodo
Publication note:
Extended appendices published as Amazon KDP eBooks
Note:
Year
Year:
2026
Version:
NENC v2.0
Date published:
22 January 2026
Planned review date:
October 2027
Region:
North East and North Cumbria
Healthcare system:
NHS
Clinical area:
Neurology
Condition:
Amyotrophic lateral sclerosis
Medicine:
Riluzole
Document type:
Shared care protocol
Indication:
Extension of survival or time to mechanical ventilation in ALS
Target population:
Adults
Intended audience:
NHS healthcare professionals
Initiating authority:
Neurological specialist services
Regulatory reference:
NICE Technology Appraisal TA20
Prescribing setting:
Secondary to primary care transfer
Monitoring requirements:
Liver function tests, full blood count, urea and electrolytes
Route of administration:
Oral or enteral
Licensing status:
Licensed for ALS only
Investigator:
[insert name of principal investigator]
Key Information:
The following is a short summary of this study to help you decide whether or not to be a part of this study. More detailed information is listed later on in this form.
Note:
Why am I being invited to take part in a research study?
You will be asked to _________ [include a high level summary of the procedures that will be done. For example:
You will be given an investigational drug and asked to come for 3 study visits. You will give a total of 3 blood samples and fill out questionnaires asking about how you feel.]
Instead of being in this research study, your choices may include:
[List alternatives procedures. For student subject pools describe alternatives for course credit. For clinical trials describe the options that you would normally offer patient. If applicable, include supportive care as an option.]
Detailed Information:
The following is more detailed information about this study in addition to the information listed above.
[Tell the subject what to expect using lay language and simple terms. Whenever appropriate include the following items:
]