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This document presents experimental findings on the expression of AhNF-YB1 and AhNF-YB10 in Arabidopsis, detailing the effects of these genes on seed development and lipid accumulation in transgenic plants.
Note: Year
Theme: Plant genetics, gene expression, transgenics
Document type: Research article
Target audience: Researchers in plant genetics and biotechnology
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Generation and analysis of transgenic mice and patient-derived xenografts for Atoh1 studies
Year: 2026
Region / Institution: University of Oregon, Sanford Research, Institut Curie, Necker Hospital, Hospital for Sick Children
Field: Molecular biology / Neuro-oncology
Document type: Research supplemental materials and methods
Organ / Institution: Transgenic Mouse Facility, Sanford Research, Institut Curie, Necker Hospital
Authors: Not specified
Target audience: Researchers in genetics and oncology
Experimental models: Transgenic mice, ES cells, patient-derived xenografts (PDX)
Animal procedures approval: Sanford Research IACUC, Institut Curie ethical committee IDFParis-Comité 1 (#2011-0012)
Human research approval: Institutional Review Board at Necker Hospital, Research Ethics Board at the Hospital for Sick Children
Techniques used: DNA constructs, transgenic mouse generation, orthotopic transplantation, bioluminescence imaging, NanoString gene expression profiling, DNA methylation analysis
Drugs / treatments: Tamoxifen, vismodegib
Mouse strains: C57BL/6J, B6.Cg-Tg(Atoh1-cre)1Bfri/J, Tg(Atoh1-cre/Esr1*)14Fsh/J, B6N.129-Ptch1tm1Hahn/J, C57BL/6-Tg(Neurod2-Smo*A1)199Jols/J, NOD.Cg-Prkdc Il2rg/SzJ, athymic nude, Tg(Lmx1a-cre)1Kjmi
Cell lines: JM8.N4 ES cells
Topics: AhNF-YB1 AhNF-YB10 transgenic