№ files_lp_4_process_2_49391
File format: docx Character count: 24545 File size: 2546 KB
Year: 2026
Region / city: Nanchang, China
Field: Biomedical research / Genetic engineering
Document type: Research methods and experimental protocol
Institution: The First Affiliated Hospital of Nanchang University
Authors: Not specified
Target audience: Researchers in genetics, xenotransplantation, and cellular biology
Experimental subjects: Pigs (SGGEP3KO), human fibroblasts, endothelial cells
Genes studied: GGTA1, CMAH, B4GALNT2, CD46, CD55, CD59, CD39, THBD, TFPI, CD47, HLA-E
Techniques used: CRISPR-Cas9, PiggyBac transfection, somatic cell nuclear transfer, flow cytometry, immunofluorescence
Cell types: Skin fibroblasts, kidney endothelial cells, cloned pig embryos
Assays: Human antibody binding, complement-dependent cytotoxicity, NK cell-mediated cytotoxicity, TAT complex formation
Culture conditions: 37–39 °C, 5% CO2, specific growth media for cell types
Period covered: Cell generation, culture, and experimental assays described in study
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