№ files_lp_4_process_3_080340
Simulation study showing outcomes of reference-based and reference-independent approaches for taxonomic identification of insects using short Illumina sequencing reads, highlighting effects of species relatedness, abundance, and sequencing depth.
Year: 2023
Methodology: In silico simulation of species identification
Technology: Illumina HiSeq 2000
Sequencing strategy: 100 bp paired-end (PE) shotgun reads
Reference library: COI barcode library (when available)
Assembly software: SOAPdenovo
Taxonomic scope: Insect mitochondrial genomes
Sample type: Mock-up pooled insect communities
Species tested: 209 complete mitochondrial genomes
Analysis depth: 100X and 1,000X sequencing coverage
Objective: Comparison of reference-based and reference-independent taxonomic identification methods
Key variables: Relatedness of species, abundance, sequencing depth, barcode length
Validation: Alignment criteria >99% identity, coverage >90% for reference detection
Price: 8 / 10 USD
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